生物技术通报 ›› 2024, Vol. 40 ›› Issue (7): 163-171.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0162

• 研究报告 • 上一篇    下一篇

甜瓜BZR转录因子家族基因的全基因组鉴定及表达模式分析

臧文蕊(), 马明, 砗根, 哈斯阿古拉()   

  1. 内蒙古大学生命科学学院 牧草与特色作物生物学教育部重点实验室,呼和浩特 010020
  • 收稿日期:2024-02-18 出版日期:2024-07-26 发布日期:2024-07-30
  • 通讯作者: 哈斯阿古拉,男,博士,教授,研究方向:植物分子生物学及基因工程;E-mail: hasiagula@imu.edu.cn
  • 作者简介:臧文蕊,女,研究方向:植物分子生物学及基因工程;E-mail: 1211589256@qq.com
  • 基金资助:
    内蒙古大学国家级大学生创新创业项目(202310126034)

Genome-wide Identification and Expression Pattern Analysis of BZR Transcription Factor Gene Family of Melon

ZANG Wen-rui(), MA Ming, CHE Gen, HASI Agula()   

  1. Key Laboratory of Herbage & Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010020
  • Received:2024-02-18 Published:2024-07-26 Online:2024-07-30

摘要:

【目的】 油菜素唑抗性因子(brassinazole-resistant,BZR)是植物特有的转录因子,对植物的生长发育起着至关重要的作用。从甜瓜全基因组范围内鉴定CmBZR基因家族成员,分析其相关基因的表达模式,为进一步探究甜瓜BZR基因家族的生物学功能提供基础。【方法】 基于甜瓜全基因组数据,通过BLAST对甜瓜BZR家族基因进行鉴定,利用生物信息学的方法分析了该家族蛋白的理化性质、基因染色体分布、基因结构、蛋白质结构域、系统进化以及启动子顺式作用元件,并利用荧光定量PCR验证BZR家族基因在甜瓜不同组织及不同生长发育时期果实中的表达情况。【结果】 甜瓜中共鉴定到6个BZR基因,系统进化分析可将其分为5个亚家族,CmBZR基因分布于第3、7、8、11和12染色体上。所有的BZR蛋白质都具有保守的结构域。CmBZR基因启动子区域显著富集与生长发育、激素信号转导和非生物逆境胁迫相关的顺式作用元件。CmBEH1-4在茎、两性花以及子房中高表达,在生长期、成熟期、呼吸跃变期和呼吸跃变后期果实中也均有表达。【结论】 在全基因组范围内从甜瓜中系统鉴定出6个甜瓜CmBZR基因家族成员,不同基因在甜瓜的各个组织和不同的生长发育时期均有表达,且表达模式存在差异。

关键词: 甜瓜, BZR, 转录因子, 全基因组鉴定, 表达分析, 生物信息学

Abstract:

【Objective】 Brassinazole-resistant(BZR)is a plant specific transcription factor that plays a crucial role in plant growth and development. Identifying members of the CmBZR gene family across the whole melon genome, and analyzing the expression patterns of related gene, may provide a basis for further exploring the biological functions of the melon BZR gene family. 【Method】 Based on the whole melon genome data, the BZR family genes in melon were identified using BLAST. Bioinformatics methods were used to analyze the physicochemical properties, chromosomal distribution, gene structure, protein domains, phylogenetic evolution, and cis acting elements of the family proteins. Fluorescence quantitative PCR was used to verify the expression of BZR family genes in different tissues and fruits of melon at different growth and development stages. 【Result】 A total of 6 BZR genes were identified in cantaloupe, which can be divided into 5 subfamilies through phylogenetic analysis. CmBZR genes are distributed on chromosomes 3, 7, 8, 11, and 12. All BZR proteins have conserved domains. The promoter region of the CmBZR gene is significantly enriched with cis-acting elements related to growth and development, hormone signal transduction, and abiotic stress. CmBEH1-4 is highly expressed in stems, female flowers, and ovaries, and is also expressed in growth, maturity, respiratory climacteric, and late respiratory climacteric stages. 【Conclusion】 Six members of the melon CmBZR gene family have been systematically identified across the whole genome, and different genes are expressed in various tissues and growth stages of the melon,and their expression patterns are various.

Key words: melon(Cucumis melo L.), BZR, transcription factor, genome-wide identification, expression analysis, bioinformatics