PCR-RFLP Analysis of mtDNA to Identify Four Kinds of Sturgeons

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.013

Abstract

Abstract: With the high efficient and accurate, molecular identification methods are widely used in some easily confused species. So we carried out the research in some miscible species of sturgeons with the application of PCR-RFLP. With the help of one pair primers, we amplified one region of mtDNA in 8 kinds of sturgeons’which contain Acipenseridae sinensis, A. schrenckii, A. baeri, A. ruthenus, Huso dauricus, H. huso, A. stellatus, A. gueldenstaeti. When digested with the restriction enzyme of Taqα I, Ava II, Eag I-HF and Nae I then test the results of PCR and enzyme digestion reactions with the density 3.0% of agarose gel, A. sinensis, A. ruthenus, H. dauricus, H. huso can be identified from other sturgeons. This method just need one pair of primers which is specific for binding to the mitochondrial DNA to amplify genomic DNA, then digested the products of PCR with different enzymes. So it is convenient to operate and can identify the species accurately in a relatively short period of time. On the basis of a little fin, we can identify common sturgeon species credibility and accuracy and also greatly improved the work efficiency.

Key words: Sturgeon, mtDNA, PCR-RFLP, Identification method, Molecular marker

Dong Chuanju, Liu Yuanyuan, Liu Xiaoyong,Song Yingnan,Xu Peng,Sun Xiaowen. PCR-RFLP Analysis of mtDNA to Identify Four Kinds of Sturgeons[J]. Biotechnology Bulletin, 2014, 0(12): 78-85.

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