Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (1): 167-172.doi: 10.13560/j.cnki.biotech.bull.1985.2015.01.025

Previous Articles     Next Articles

Preparation of Algae Agents for S. miltiorrhiza Bunge Root Rot Biocontrol Bacillus subtilis2-1

Zhou Ying, Yuan Mengjuan, Han Jun, Chen Fang   

  1. (School of Pharmaceutical,Liaocheng University,Liaocheng 252059)
  • Received:2014-09-28 Online:2015-01-09 Published:2015-01-10

Abstract: With algae residue as carrier, S. miltiorrhiza bunge root rot biocontrol agents: Bacillus subtilis2-1(CGMCC No.9256), were developed into algae agents, which features both nutritional and cell biological control function, also in line with the basic indicators of agricultural products, technical indicators microbial agents. Then appropriate ratio of seaweed broth and residue was chosen by physical means. Besides, the number of viable cells, moisture content, pH value and expiration date were measured by agricultural product quality testing methods, and field experiments was conducted to test biocontrol effect. Results showed that number of effective viable agents was 2.32×109/g, moisture content was 6.75%, pH value was 6.7, and the expiration date was about 11.6 months , all of which were in line with the technical specifications of the particles. The incidence of root rot in field trials rate decreased 37.6% compared with the blank control group, the effect of biocontrol was obvious.

Key words: root rot, biocontrol, algae agents, preparation