Effect of Root Rot on Arbuscular Mycorrhizal Fungi Community in Root Zone Soil of Lycium barbarum L.

doi:10.13560/j.cnki.biotech.bull.1985.2021-0190

Abstract

Abstract:

This paper aims to explore the effects of root rot pathogen infection on the community composition of arbuscular mycorrhizal fungi（AMF）in the root zone of Lycium barbarum L.,for laying a foundation in later applying AMF to control the root rot of L. barbarum L. The roots and soil samples of healthy and infected L. barbarum L. in three cultivation sites（Yinchuan and Zhongning county of Ningxia Hui Autonomous Region,and Jingyuan county of Gansu province）were collected. Wet sieve precipitation method and AMF colonization staining method were used to analyze the spore composition and density,mycorrhizal infection rate of AMF and the correlation between AMF diversity and soil physical and chemical factors. The results demonstrated that there was no significant difference in spore density,total mycorrhizal infection rate and T-GRSP content after the healthy L. barbarum L. plants were invaded by the pathogen of root rot,while the EE-GRSP content in Zhongning county significantly increased. The AMF community composition in the root zone soil of healthy and infected L. barbarum L. plants in different sites varied significantly（R ²=0.875,P=0.001）. The dominant genus and species in Zhongning and Jingyuan county was changed,while that in Yinchuan site was unchanged. The root rot changed the soil AMF community structure in the root area of L. barbarum L. in Ningxia,such as the α-diversity increasing in Yinchuan,but no impact on the α-diversity in Zhongning and Jinghuan. There were significance among AMF spore density,species abundance,total mycorrhizal infection rate and soil physicochemical properties,and soil physicochemical properties did not affect the species diversity of AMF,but affected AMF colonization.

Key words: Lycium barbarum L., root rot, arbuscular mycorrhizal fungi, diversity

LV Yan, WANG Wen-bin, GOU Qi, WANG Ying-na, LI Jing-yu, LIU Jian-li. Effect of Root Rot on Arbuscular Mycorrhizal Fungi Community in Root Zone Soil of Lycium barbarum L.[J]. Biotechnology Bulletin, 2021, 37(12): 29-40.

Figures/Tables 10

References 30

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土壤理化性质 Soil physicochemical properties	银川Yinchuan		中宁Zhongning		靖远Jingyuan
土壤理化性质 Soil physicochemical properties	健康树 Healthy tree	病树 Root rot-infected tree	健康树 Healthy tree	病树 Root rot-infected tree	健康树 Healthy tree	病树 Root rot-infected tree
有机质OM/（g·kg^-1）	12.82±0.64^b	20.18±0.98^a	12.83±2.07^a	12.12±1.1^a	11.42±0.94^a	11.92±1.52^a
全磷TP/（g·kg^-1）	0.7±0.07^a	0.96±0.37^a	1.33±0.35^a	1.25±0.32^a	0.95±0.12^a	0.54±0.29^a
硝态氮/NO₃^--N（mg·kg^-1）	6.06±0.84^a	4.58±0.39^b	6.71±0.13^a	4.82±0.43^b	4.95±0.29^b	6.9±0.05^a
氨态氮/NH₄⁺-N（mg·kg^-1）	0.41±0.07^a	0.23±0.17^a	0.39±0.12^a	0.53±0.03^a	0.41±0.32^a	0.32±0.16^a
全氮TN/（g·kg^-1）	1.29±0.2^a	1.56±0.32^a	1.05±0.17^a	1.36±0.05^a	1.09±0.12^a	1.26±0.09^a
全钾TK/（g·kg^-1）	2.72±0.05^a	2.55±0.09^a	2.15±0.51^a	2.45±0.26^a	2.65±0.16^b	3.94±0.26^a
速效钾AK/（mg·kg^-1）	144.08±5.71^a	107.96±10^a	114.43±18.84^a	155.02±55.15^a	145.06±6.98^a	136.47±2.62^a
速效磷AP/（mg·kg^-1）	7.29±2.24^a	6.75±1.64^a	6.22±1.43^a	7.25±1.39^a	4.56±2.71^a	2.98±0.54^a
pH	7.78±0.09^a	7.68±0.18^a	7.59±0.04^b	7.9±0.08^a	8.04±0.22^a	8.24±0.1^a

土壤理化性质 Soil physicochemical properties	银川Yinchuan		中宁Zhongning		靖远Jingyuan
土壤理化性质 Soil physicochemical properties	健康树 Healthy tree	病树 Root rot-infected tree	健康树 Healthy tree	病树 Root rot-infected tree	健康树 Healthy tree	病树 Root rot-infected tree
有机质OM/（g·kg^-1）	12.82±0.64^b	20.18±0.98^a	12.83±2.07^a	12.12±1.1^a	11.42±0.94^a	11.92±1.52^a
全磷TP/（g·kg^-1）	0.7±0.07^a	0.96±0.37^a	1.33±0.35^a	1.25±0.32^a	0.95±0.12^a	0.54±0.29^a
硝态氮/NO₃^--N（mg·kg^-1）	6.06±0.84^a	4.58±0.39^b	6.71±0.13^a	4.82±0.43^b	4.95±0.29^b	6.9±0.05^a
氨态氮/NH₄⁺-N（mg·kg^-1）	0.41±0.07^a	0.23±0.17^a	0.39±0.12^a	0.53±0.03^a	0.41±0.32^a	0.32±0.16^a
全氮TN/（g·kg^-1）	1.29±0.2^a	1.56±0.32^a	1.05±0.17^a	1.36±0.05^a	1.09±0.12^a	1.26±0.09^a
全钾TK/（g·kg^-1）	2.72±0.05^a	2.55±0.09^a	2.15±0.51^a	2.45±0.26^a	2.65±0.16^b	3.94±0.26^a
速效钾AK/（mg·kg^-1）	144.08±5.71^a	107.96±10^a	114.43±18.84^a	155.02±55.15^a	145.06±6.98^a	136.47±2.62^a
速效磷AP/（mg·kg^-1）	7.29±2.24^a	6.75±1.64^a	6.22±1.43^a	7.25±1.39^a	4.56±2.71^a	2.98±0.54^a
pH	7.78±0.09^a	7.68±0.18^a	7.59±0.04^b	7.9±0.08^a	8.04±0.22^a	8.24±0.1^a

样地 Sites		孢子密度 Spore density/（Spores·g^-1）soil	总侵染率 Total colonization rate/%	易提取球囊霉素 EEG/（mg·g^-1）	总球囊霉素 TG/（mg·g^-1）
银川 Yinchuan	健康树 Healthy tree	9.77±0.40^a	2.50±1.21^a	20.71±4.98^a	57.16±10.33^a
银川 Yinchuan	病树 Root rot-infected tree	9.70±1.67^a	7.72±4.03^a	29.37±5.03^a	70.50±2.37^a
中宁 Zhongning	健康树 Healthy tree	34.90±11.47^a	10.27±7.69^a	18.09±2.77^b	61.74±7.26^a
中宁 Zhongning	病树 Root rot-infected tree	36.40±12.25^a	17.85±9.80^a	24.42±2.75^a	67.65±5.48^a
靖远 Jingyuan	健康树 Healthy tree	36.30±13.13^a	30.00±3.27^a	12.54±1.46^a	50.50±6.98^a
靖远 Jingyuan	病树 Root rot-infected tree	42.67±10.16^a	33.23±1.00^a	22.42±8.11^a	55.76±15.49^a

样地 Sites		孢子密度 Spore density/（Spores·g^-1）soil	总侵染率 Total colonization rate/%	易提取球囊霉素 EEG/（mg·g^-1）	总球囊霉素 TG/（mg·g^-1）
银川 Yinchuan	健康树 Healthy tree	9.77±0.40^a	2.50±1.21^a	20.71±4.98^a	57.16±10.33^a
银川 Yinchuan	病树 Root rot-infected tree	9.70±1.67^a	7.72±4.03^a	29.37±5.03^a	70.50±2.37^a
中宁 Zhongning	健康树 Healthy tree	34.90±11.47^a	10.27±7.69^a	18.09±2.77^b	61.74±7.26^a
中宁 Zhongning	病树 Root rot-infected tree	36.40±12.25^a	17.85±9.80^a	24.42±2.75^a	67.65±5.48^a
靖远 Jingyuan	健康树 Healthy tree	36.30±13.13^a	30.00±3.27^a	12.54±1.46^a	50.50±6.98^a
靖远 Jingyuan	病树 Root rot-infected tree	42.67±10.16^a	33.23±1.00^a	22.42±8.11^a	55.76±15.49^a

属 Genus	种 Species	银川Yinchuan		中宁Zhongning		靖远Jingyuan
属 Genus	种 Species	健康树 Healthy tree	病树Root rot-infected tree	健康树 Healthy tree	病树Root rot-infected tree	健康树 Healthy tree	病树Root rot-infected tree
无梗囊霉属 Acaulospora	双网无梗囊霉A. bireticulata	37.16±3.61	34.58±1.59	-	16.83±0.57	34.89±1.94	34.19±1.09
	椒红无梗囊霉A. capsicula	17.46±1.67	52.9±1.66	-	16.67±0.29	51.77±0.69	52.47±0.18
	凹坑无梗囊霉A. excavate	51.41±0.47	-	0.61±0.53	33.97±0.85	51.64±0.31	57.75±0.51
	浅窝无梗囊霉A. lacunosa	-	-	0.29±0.51	33.87±0.79	-	-
	光壁无梗囊霉A. laevis	16.83±0.58	34.05±1.21	10.83±4.31	56.93±1.00	34.22±1.30	-
	蜜色无梗囊霉A. mellea	52.49±2.12	51.84±0.91	5.28±0.83	-	16.61±0.19	16.59±0.15
	皱襞无梗囊霉A. rugosa	33.54±0.49	-	4.13±4.66	52.59±1.41	34.76±2.18	-
	细凹无梗囊霉A. scrobiculata	-	54.48±2.43	-	-	55.39±1.32	61.01±0.41
	孢果无梗囊霉A. sporocarpia	16.72±0.37	-	0.15±0.26	-	-	33.3±0.32
近明球囊霉属 Claroideoglomus	近明球囊霉C. claroideum	-	-	0.29±0.51	35.11±1.83	-	-
	待霄草近明球囊霉C. drummondii	-	-	-	16.59±0.15	-	-
	层状近明球囊霉C. lamellosum	-	-	0.6±0.52	33.44±0.40	16.61±0.19	-
	纯黄近明球囊霉C. luteum	-	-	2.91±3.25	34.67±1.46	16.62±0.21	-
球囊霉属 Glomus	卷曲球囊霉G. convolutum	-	34.57±1.56	2.09±2.02	51.36±0.77	-	-
	团集球囊霉G. glomerulatum	-	-	0.15±0.26	16.67±0.30	-	-
	海得拉巴球囊霉G. hyderabadensis	-	16.9±0.69	-	-	-	-
	大果球囊霉G. macrocarpum	17.15±1.12	33.66±0.60	6.19±1.61	-	58.09±1.46	54.93±0.40
	宽柄球囊霉G. magnicaule	-	-	0.15±0.26	-	-	-
	黑球囊霉G. melanosporum	57.42±2.28	54.87±1.12	11.51±2.48	54.51±2.26	56.16±1.66	54.5±0.19
	多梗球囊霉G. multicaule	33.65±0.57	52.63±0.43	-	-	16.61±0.19	-
	具疱球囊霉G. pustulatum	-	-	1.68±2.17	-	-	-
	网状球囊霉G. reticulatum	59.15±3.05	52.5±1.40	1.98±1.49	16.68±0.31	55.36±1.31	54.44±1.03
	G. sp1	-	-	-	-	52.7±0.19	-
	G. sp2	52.37±2.12	-	-	-	-	-
	G. sp3	-	-	-	-	55.26±1.33	53.67±1.42
	扭形球囊霉G. tortuosum	-	56.58±0.97	1.68±2.53	-	-	-
	地表球囊霉G. versiforme	67.25±4.38	64.72±2.28	10.61±2.93	53.74±1.95	34.51±1.33	51.9±0.65
根孢囊霉属 Rhizophagus	明根孢囊霉R. clarus	33.65±0.57	16.7±0.34	21.26±3.06	64.67±1.60	-	16.59±0.15
盾巨孢囊霉属 Scutellospora	网纹盾巨孢囊霉S. reticulata	-	16.9±0.69	1.22±1.06	51.28±0.66	-	33.52±0.68
隔球囊霉属 Septoglomus	缩隔球囊霉S. constrictum	34.75±1.64	53.16±1.39	13.53±5.71	57.6±1.99	55.03±2.40	51.36±0.60
隔球囊霉属 Septoglomus	沙荒隔球囊霉S. deserticola	16.82±0.56	16.76±0.46	-	33.51±0.52	-	54.85±0.90
	黏质隔球囊霉S. viscosum	-	-	2.85±1.38	-	50.61±0.32	50.61±0.33
	种数 Number of species	16	17	22	19	18	16