Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (5): 32-40.doi: 10.13560/j.cnki.biotech.bull.1985.2015.05.006

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Research Progress of Enzyme and Relevant Gene Screened from Rumen by Metagenomic Approach

Zhang Jun1, Zhao Shengguo1, Wang Jiaqi1, Jin Di1, Bu Dengpan1,2,3   

  1. (1. State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193;2. World Agroforestry Centre,East and Central Asia,Beijing 100193;3. Synergetic Innovation Center of Food Safety and Nutrition,Harbin 150030)
  • Received:2014-08-26 Online:2015-05-18 Published:2015-05-18

Abstract: Rumen microbial community is a complex biological system with extremely rich biodiversity and large amount of genetic and ecological resources, and it is also an important treasury for the development of enzyme. Using metagenomic approach could avoid the restriction of microbial culturing, with directly extracting DNA, gene screening and expressing the uncultured microbes to enlarge the database of genes, which provides possibility for the development of novel biocatalyst. The study explained metagenomic approach, and summarized the application of metagenomic approach in screening functional enzymes and studying relevant genes from rumen.

Key words: metagenomic, rumen, screening, enzyme