Effect of Phosphate-solubilizing Bacteria on Oxidative Response of Pleurotus eryngii Under Lead Stress

doi:10.13560/j.cnki.biotech.bull.1985.2016.05.025

Abstract

Abstract: In this study Pleurotus eryngii was selected to enrich Pb2+，and 8 strains with capability against Pb2+ of 800 mg/L were screened from the strain library of our laboratory. Two strains CG8 and FFT1 with high phosphate-solubilizing capacity，as well as FFC6 and FFC11with low phosphate-solubilizing capacity were selected. Using 16S rDNA analysis，CG8 was identified as Klebsiella sp.，FFT1 as Pseudomonas sp.，FFC6 and FFC11 as Bacillus sp.；the similarity of their sequences reached 99%. Further，the effects of 4 strains of phosphate-solubilizing bacterium（PSB）on the mycelium growth，Pb accumulation，lipid peroxidation，the content of sulfhydryl-containing protein，and antioxidant enzyme of P. eryngii in Pb-contaminating liquid medium were investigated. The result showed that inoculation with PSB enhanced the hyphal biomass of P. eryngii and accumulation of heavy metals. Moreover，the content of MAD，and the activities of SOD and POD in mycelium decreased significantly，while the content of total sulfhydryl（TSH）increased. All results confirmed that PSB efficiently reduced the toxicity of heavy metals to P. eryngii mycelium and the oxidative stress induced by heavy metals. CG8 with the strongest capacity of solubilizing phosphate had more significant effects to resist the mycelium accumulating heavy metals and oxidative stress induced by heavy metals in P. eryngii，the accumulation of Pb increasing 96.1% and the PB-SH increasing 91.3%.

Key words: mushroom, heavy metal, phosphate-solubilizing capacity, antioxidant system

HE Nan,XU Heng. Effect of Phosphate-solubilizing Bacteria on Oxidative Response of Pleurotus eryngii Under Lead Stress[J]. Biotechnology Bulletin, 2016, 32(5): 187-193.

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