Effect and Mechanism of Selenium Nanoparticle Against Islet β Cell Apoptosis

doi:10.13560/j.cnki.biotech.bull.1985.2016.11.035

Abstract

Abstract: The purpose of this research was to study the effect and molecular mechanism of selenium nanoparticles（SeNPs）in the treatment of islet β cell apoptosis in type 2 diabetes mellitus. Electron microscope and nanoparticle size analyzer were used to identify SeNPs surface morphology,and relevant molecular biological techniques were used to validate the protection effect and mechanism of SeNPs on islet β cells apoptotic model. Results showed that the surface charge of selenium nanoparticle decorated by chitosan（CS）increased to 24.8 mv,and its storage time was extended to over 100 days. Cytology results showed that SeNPs under 2 μmol/L had no toxicity. Meanwhile,SeNPs in 38.1 nmol/L activated TrxR activity and reduced intracellular ROS level,which led to apoptotic model cells survival rate significantly increased 31.75%. Furthermore,the animal experiments also verified that SeNPs could resist the apoptosis of islet cell β. In conclusion,SeNPs may eliminate the apoptosis of islet cell β in type 2 diabetes mellitus,which shows a potential therapeutic activity in the treatment of type 2 diabetes.

Key words: selenium nanoparticle, type 2 diabetes mellitus, oxidative stress, molecular mechanism

RAO Lei, RAO Min, QIU Hong-hong, LI Hong-hui, LIU Jing, ZHUANG Man-jiao. Effect and Mechanism of Selenium Nanoparticle Against Islet β Cell Apoptosis[J]. Biotechnology Bulletin, 2016, 32(11): 271-277.

References

[1] MacFarlane WM, Chapman JC, Shepherd RM, et al. Engineering a glucose-responsive human insulin-secreting cell line from islets of Langerhans isolated from a patient with persistent hyperinsulinemic hypoglycemia of infancy[J]. Journal of Biological Chemistry, 1999, 274（48）：34059-66.
[2] Rayman MP. The importance of selenium to human health[J]. The Lancet, 2000, 356（9225）：233-241.
[3] Gao X, Z hang J, Zhang L. Hollow sphere selenium nanoparticles：their in-vitro antihydroxyl radical effect[J]. Advanced Materials, 2002, 14（4）：290.
[4] Gates B, Mayers B, Cattle B, et al. Synthesis and characterization of uniform nanowires of trigonal selenium[J]. Advanced Functional Materials, 2002, 12（3）：219.
[5] Wang H, Zhang J, Yu H. Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on seleno enzymes：comparison with selenomethionine in mice[J]. Free Radical Biology and Medicine, 2007, 42（10）：1524-1533.
[6] Tran PA, Webster TJ. Selenium nanoparticles inhibit Staphylococcus aureus growth[J]. Int J Nanomedicine, 2011, 6：1553.
[7] Li YH, Li XL, Wong YS, et al. The reversal of cisplatin-induced nephrotoxicity by selenium nanoparticles functionalized with 11-mercapto-1-undecanol by inhibition of ROS-mediated apoptosis[J]. Biomaterials, 2011, 32（34）：9068-9076.
[8] Torres S, Campos V, León C, et al. Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity[J]. Journal of Nanoparticle Research, 2012, 14（11）：1-9.
[9] Navarro-Alarcon M, López-G dela Serrana H, Perez-Valero V. Serum and urine selenium concentrations as indicators of body status in patients with diabetes mellitus[J]. Science of the Total Environment, 1999, 228（1）：79-85.
[10] Kljai K, Runje R. Selenium and glycogen levels in diabetic patients[J]. Biol Trace Elem Res, 2001, 83（3）：223-229.
[11] Brown K, Pickard K, Nicol F, et al. Effects of organic and inorganic selenium supplementation on selenoenzyme activity in blood lymphoctyes, granulocytes, platelets and erythrocytes[J]. Clinical Science, 2000, 98（5）：593-599.
[12] Steinbrenner H, Sies H. Protection against reactive oxygen species by selenoproteins[J]. Biochimica et Biophysica Acta（BBA）-General Subjects, 2009, 1790（11）：1478-1485.
[13] Rajalakshmi M, Arora A. Optical properties of selenium nanopart-icles dispersed in polymer[J]. Solid State Communications, 1999, 110（2）：75-80.
[14] Prabaharan M. Review paper：chitosan derivatives as promising materials for controlled drug delivery[J]. Journal of Biomaterials Applications, 2008, 23（1）：5-36.
[15] Yu B, Z hang Y, Z heng W, et al. Positive surface charge enhances selective cellular uptake and anticancer efficacy of selenium nanoparticles[J]. Inorg Chem, 2012, 51（16）：8956-63.
[16] Karaskov E, Scott C, Zhang L, et al. Chronic palmitate but not oleate exposure induces endoplasmic reticulum stress, which may contribute to INS-1 pancreatic cell apoptosis[J]. Endocrinology, 2006, 147：3398-3407.
[17] Cunha DA, Hekerman P, Ladriere L, et al. Initiation and execution of lipotoxic ER stress in pancreatic cells[J]. Journal of Cell Science, 2008, 121：2308-2318.
[18] Eizirik DL, Cardozo AK, Cnop M, et al. The role for endo-plasmicreticulum stress in diabetes mellitus[J]. Endocrine Reviews, 2008, 29（1）：42-61.