Research Progress on FEN1-mediated Functional Nucleic Acid Biosensors

doi:10.13560/j.cnki.biotech.bull.1985.2019-0224

Abstract

Abstract: Flap endonuclease 1（FEN1）is a structure-specific enzyme that can recognize and efficiently and stably cleave the triplex DNA structures to release 5'-flap fragments. Due to its specific characteristic,FEN1 is nowadays widely used for amplification detection of DNA,RNA,and viruses through different signal output modes. This review firstly provides an overview regarding the discovery history,specific properties and comprehensive effects of FEN1,followed by detailed classification of the FEN1-mediated biosensors in terms of the target substances including the detections of single nucleotide polymorphism,methylation,genotype,RNA,virus,tumor and microbe. In addition,this review describes the uses of FEN-1 for in vivo characterization and treatment by combining with nanomaterials. Moreover,the review compares and evaluates different FEN1-mediated biosensors with respect to working principle,sensitivity,specificity and application fields. Finally,the review highlights the challenges and the future research perspectives of FEN1-mediated biosensors,aiming at facilitating the development of a more convenient,sensitive and accurate FEN1-mediated biosensor for functional nucleic acid detection in the future.

Key words: FEN1, triplex DNA structure, signal amplification, biosensor, endonuclease

SUN Yu-ge, LI Chen-wei, DU Zai-hui, XU Wen-tao. Research Progress on FEN1-mediated Functional Nucleic Acid Biosensors[J]. Biotechnology Bulletin, 2020, 36(4): 208-224.

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