Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (5): 145-152.doi: 10.13560/j.cnki.biotech.bull.1985.2017.05.021

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Cloning and Tissue Expression Analysis of NITR in Nile Tilapia(Oreochromis niloticus)

WANG Zhi-wen HUANG Yu ZHANG Hai-yan TANG Ju-fen JIAN Ji-chang LU Yi-shan   

  1. Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemilogy for Aquatic Economic Animals,Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes,Fisheries College of Guangdong Ocean University,Zhanjiang 524088
  • Received:2016-11-06 Online:2017-05-25 Published:2017-05-19

Abstract: The encoding sequence of NITR(Novel immune-type receptor)gene(GenBank accession number:KX989509;designated as On-NITR)was cloned from the spleen of Nile tilapia(Oreochromis niloticus). The complete cDNA sequence of On-NITR gene was 1 119 bp with an ORF of 1 026 bp encoding 341 amino acids. The deduced amino acid sequence of On-NITR had an estimated molecular weight of 37.38 kD and a theoretical pI of 8.28. Through NCBI BLAST,we found the amino acid sequence identities between On-NITR and previously reported fish NITRs were approximately 27% - 46%. Amino acid alignment indicated that On-NITR consisted of one typical signal peptide,two extracellular immunoglobulin(Ig)domains(V and V/C2),one transmembrane domain,and one highly conserved the cytoplasmic region with an immunoreceptor tyrosine-based inhibitor motif(ITIM)and an ITIM-like motif(itim). Moreover,the analysis by real time quantitative PCR revealed that the expression of On-NITR was detected in all examined tissues of a healthy Nile tilapia with the higher expression level in intestine,skin and liver,while lower expression in thymus,gill,spleen,heart,and brain as well as the lowest level in head and kidney.

Key words: Nile tilapia(Oreochromis niloticus), NITR protein, gene cloning, tissue expression analysis