Research Advances on Integrating Metagenomics and Synthetic Biology in Discovering Novel Biocatalysts

doi:10.13560/j.cnki.biotech.bull.1985.2018-0027

Abstract

Abstract: Biocatalysts refer to cells or enzymes with catalytic activity,and they are widely applied in industry,agriculture,medicine,and energy fields due to their high efficiency and environmentally friendly. Traditional biocatalyst mining technology relies on bio-separation and in vitro culture technology,which to a certain degree hinders the development of novel biocatalysts. Discovering novel biocatalysts by metagenomics is as such,directly extracting the DNA of all microorganisms from the target sample,constructing genomic library,and then screening novel biocatalysts based on functional activity and sequence analysis,which avoids the necessity of isolation and laboratory cultivation of microorganisms. Synthetic biology refers to redesign biological pathways,organisms or biological systems via the design,assembly and construction of components by employing engineering principles. This paper reviews the advances on minng new biocatalysts by metagenomics methods and discusses the efficiency of synthetic biology approaches to improve biocatalyst identification in metagenomic library screening,aiming to improve the efficiency of mining new biocatalysts.

Key words: biocatalyst, metagenomics, activity screening, sequence-based screening, synthetic biology

WANG Ye, JIA Zhen-hua, SONG Shui-shan. Research Advances on Integrating Metagenomics and Synthetic Biology in Discovering Novel Biocatalysts[J]. Biotechnology Bulletin, 2018, 34(8): 35-42.

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