Research Progress on Magnetosome-mediated Biosensors

doi:10.13560/j.cnki.biotech.bull.1985.2018-0223

Abstract

Abstract: Bacterial magnetosomes（BMs）are special organelles synthesized by magnetotactic bacteria（MTB）and used for geomagnetic navigation in their aquatic habitat. It consists of an external lipid bilayer membrane and internal magnetic nanocrystals. Because of its narrow particle size distribution,uniform morphology,single magnetic domain,paramagnetism,large specific surface area,and high biocompatibility,it is widely used in the medical field as an anticancer drug delivery vehicle,nuclear magnetic resonance imaging contrast agent and imaging probe,etc. At present,there are relatively few researches on the preparation of biosensors based on BMs and applied in the biological detection technology field,and they are mainly biased toward the covalent or non-covalent modification of antibodies on the surface of BMs membranes,and the use of antigen-antibody-specific immunoreactions to achieve the detection of target substances. This article reviews the structure and composition,basic characteristics,extraction and purification,morphological structure characterization and application of BMs in the enrichment of target substances,and highlights several types of BMs-based biosensors such as electrochemical biosensors,fluorescent biosensors,magnetic biosensors,etc. Finally,the practical significance and existing problems of magnetosome-mediated biosensors in application research are discussed. The prospects for the development of magnetosome-mediated biosensors and the opportunities and challenges that may be faced are anticipated in order to provide reference for promoting the application of magnetosome-mediated biosensors in practice.

Key words: magnetosomes, nanomaterials, biosensors, enrichment

LI Shu-ting, ZHOU Zi-qi, TIAN Jie-sheng, XU Wen-tao. Research Progress on Magnetosome-mediated Biosensors[J]. Biotechnology Bulletin, 2018, 34(9): 70-78.

References

[1] Faivre D, Schuler D.Magnetotactic bacteria and magnetosomes[J]. Chem Rev, 2008, 108(11):4875-4898.
[2] Jacob JJ, Suthindhiran K.Magnetotactic bacteria and magnetosomes-Scope and challenges[J]. Materials Science and Engineering:C, 2016, 68:919-928.
[3] Erdal E, Demirbilek M, Yeh Y, et al.A Comparative study of receptor-targeted magnetosome and HSA-coated iron oxide nanoparticles as MRI contrast-enhancing agent in animal cancer model[J]. Appl Biochem Biotechnol, 2018, 185(1):91-113.
[4] Sun J, Li Y, Liang XJ, et al.Bacterial magnetosome:a novel biogenetic magnetic targeted drug carrier with potential multifunctions[J]. J Nanomater, 2011(2011):469031-469043.
[5] Alphandéry E.Applications of magnetosomes synthesized by magnetotactic bacteria in medicine[J]. Frontiers in Bioengineering and Biotechnology, 2014, 2:5.
[6] Ceyhan B, Alhorn P, Lang C, et al.Semisynthetic biogenic magnetosome nanoparticles for the detection of proteins and nucleic acids[J]. Small, 2006, 2(11):1251-1255.
[7] Chen JF, Li Y, Wang ZF, et al.High-sensitivity detection of fruit tree viruses using bacterial magnetic particles[J]. Journal of Integrative Plant Biology, 2009, 51(4):409-413.
[8] Xiang Z, Yang X, Xu J, et al.Tumor detection using magnetosome nanoparticles functionalized with a newly screened EGFR/HER2 targeting peptide[J]. Biomaterials, 2017, 115:53-64.
[9] Boucher M, Geffroy F, Prévéral S, et al.Genetically tailored magnetosomes used as MRI probe for molecular imaging of brain tumor[J]. Biomaterials, 2017, 121:167-178.
[10] Alphandéry E, Guyot F, Chebbi I.Preparation of chains of magnetosomes, isolated from Magnetospirillum magneticum strain AMB-1 magnetotactic bacteria, yielding efficient treatment of tumors using magnetic hyperthermia[J]. International Journal of Pharmaceutics, 2012, 434(1-2):444-452.
[11] Lins U, Freitas F, Keim CN, et al.Electron spectroscopic imaging of magnetotactic bacteria:magnetosome morphology and diversity[J]. Microscopy and Microanalysis, 2000, 6(5):463-470.
[12] Grünberg K, Müller EC, Otto A, et al.Biochemical and proteomic analysis of the magnetosome membrane in Magnetospirillum gryphiswaldense[J]. Applied and Environmental Microbiology, 2004, 70(2):1040-1050.
[13] Nakamura N, Burgess JG, Yagiuda K, et al.Detection and removal of Escherichia coli using fluorescein isothiocyanate conjugated monoclonal antibody immobilized on bacterial magnetic particles[J]. Anal Chem, 1993, 65(15):2036-2039.
[14] Xu J, Hu J, Liu L, et al.Surface expression of protein A on magnetosomes and capture of pathogenic bacteria by magnetosome/antibody complexes[J]. Frontiers in Microbiology, 2014, 5:136.
[15] 刘珺, 周培国. 趋磁细菌应用于重金属废水处理的研究进展[J]. 环境科技, 2008, 21(6):60-63.
[16] Li K, Chen C, Chen C, et al.Magnetosomes extracted from Magnetospirillum magneticum strain AMB-1 showed enhanced peroxidase-like activity under visible-light irradiation[J]. Enzyme and Microbial Technology, 2015, 72:72-78.
[17] Xiang L, Wei J, Jianbo S, et al.Purified and sterilized magnetosomes from Magnetospirillum gryphiswaldense MSR-1 were not toxic to mouse fibroblasts in vitro[J]. Letters in Applied Microbiology, 2007, 45(1):75-81.
[18] Sun J, Tang T, Duan J, et al.Biocompatibility of bacterial magnet-osomes:acute toxicity, immunotoxicity and cytotoxicity[J]. Nanotoxicology, 2010, 4(3):271-283.
[19] Revathy T, Jayasri MA, Suthindhiran K.Toxicity assessment of magnetosomes in different models[J]. 3 Biotech, 2017, 7(2):126.
[20] Guo F, Liu Y, Chen Y, et al.A novel rapid and continuous procedure for large-scale purification of magnetosomes from Magnetospirillum gryphiswaldense[J]. Applied Microbiology and Biotechnology, 2011, 90(4):1277-1283.
[21] Pi F, Sun J, Liu W, et al.Elimination of aflatoxin B1 in vegetable oil based on immuno-magnetosomes probes from a novel magnetotactic bacterium[J]. Food Control, 2017, 80:319-326.
[22] Wu L, Gao B, Zhang F, et al.A novel electrochemical immunosen-sor based on magnetosomes for detection of staphylococcal enterotoxin B in milk[J]. Talanta, 2013, 106:360-366.
[23] Mann S, Moench TT, Williams RJP.A high resolution electron microscopic investigation of bacterial magnetite. implications for crystal growth[J]. Proceedings of the Royal Society of London, 1984, 221(1225):385-393.
[24] Feng S, Wang L, Palo P, et al.Integrated self-assembly of the Mms6 magnetosome protein to form an iron-responsive structure[J]. International Journal of Molecular Sciences, 2013, 14(7):14594-606.
[25] Yamamoto D, Taoka A, Uchihashi T, et al.Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy[J]. Proc Natl Acad Sci, 2010, 107(20):9382-9387.
[26] Li A, Zhang H, Zhang X, et al.Rapid separation and immunoassay for low levels of Salmonella in foods using magnetosome-antibody complex and real-time fluorescence quantitative PCR[J]. Journal of Separation Science, 2010, 33(21):3437-3443.
[27] Huang J, Guo L, Zheng L M.Rapid enrichment and determination of phosphopeptides using bacterial magnetic particles via both strong and weak interactions[J]. Analyst, 2010, 135(3):559-563.
[28] Xiong K, Wei W, Jin Y, et al.Biomimetic immuno-magnetosomes for high-performance enrichment of circulating tumor cells[J]. Adv Mater, 2016, 28(36):7929-7935.
[29] Ota H, Takeyama H, Nakayama H, et al.SNP detection in transforming growth factor-β1 gene using bacterial magnetic particles[J]. Biosens Bioelectron, 2003, 18(5-6):683-687.
[30] Amemiya Y, Tanaka T, Yoza B, et al.Novel detection system for biomolecules using nano-sized bacterial magnetic particles and magnetic force microscopy[J]. J Biotechnol, 2005, 120(3):308-314.
[31] Sun X, Wu L, Ji J, et al.Longitudinal surface plasmon resonance assay enhanced by magnetosomes for simultaneous detection of Pefloxacin and Microcystin-LR in seafoods[J]. Biosens Bioelectron, 2013, 47:318-323.
[32] Wacker R, Ceyhan B, Alhorn P, et al.Magneto immuno-PCR:a novel immunoassay based on biogenic magnetosome nanoparticles[J]. Biochemical and Biophysical Research Communications, 2007, 357(2):391-396.