Screening,Identification and Biocontrol Effect of Antagonistic Bacteria on Potato Common Scab

doi:10.13560/j.cnki.biotech.bull.1985.2018-0417

Abstract

Abstract:

Potato common scab is one of the major diseases of potato,and using biological means to control it gradually attracts researchers' attentions. In order to obtain a strain with strong antagonism to the pathogen of potato common scab,using Streptomyces bottropensis AMCC400023,a pathogen of potato common scab as a target strain,an antagonistic strain was isolated from the soil collected from the potato-planting area in Gaomi City of Shandong Province,and the plate confrontation growth method and the Oxford cup test were used for primary screening and rescreening. As results,a strain 1-3-4 with strong antagonistic effect was obtained,the diameter of the inhibition zone was 19.74 mm,and the control effect in pot experiment reached 40.27% and 48.66%. According to the 16S rDNA sequence analysis of strain 1-3-4,its culture morphological characteristics,as well as physiological and biochemical characteristics,it was identified as Bacillus methylotrophicus. The results of pots experiment showed that strain 1-3-4 had a promising control effect on potato common scab and had fine application prospects.

Key words: potato common scab, biological control, antagonistic bacteria, Bacillus methylotrophicus

LI Yu-cong, Li Bin-ying, YOU Xin-yi, LIU Yu-hao, ZHOU Bo, LIN Rong-shan. Screening,Identification and Biocontrol Effect of Antagonistic Bacteria on Potato Common Scab[J]. Biotechnology Bulletin, 2018, 34(10): 116-121.

References

[1] Loria R, Bukhalid RA, Fry BA, et al.Plant pathogenicity in the genus Streptomyces[J]. Plant Disease, 1997, 81(8):836-846.
[2] Hiltunen LH, Kelloniemi J, Valkonen JPT.First report of Streptomyces europaeiscabiei causing common scab on potato in Finland[J]. Plant Disease, 2014, 98(9):1267-1267.
[3] Zhou B, Zhang MS, Ma XK.First Report of Streptomyces bottropensis causing potato common Scab in Hebei province, China[J]. Plant Disease, 2016, 101(3).
[4] Sarwar A, Latif Z, Cabaleiro C.First report of Streptomyces turgidiscabies causing potato common scab in Spain[J]. Plant Disease, 2017, 101(9).
[5] 赵伟全, 杨文香, 刘大群, 等. 中国马铃薯疮痂病研究初报[J]. 河北农业大学学报, 2004, 27(6):74-77.
[6] Hiltunen LH, Laakso I, Chobot V, et al.Influence of thaxtomins in different combinations and concentrations on growth of micropropagated potato shoot cultures[J]. Journal of Agricultural & Food Chemistry, 2006, 54(9):3372-3379.
[7] King RR, Lawrence CH, Clark MC.Correlation of phytotoxin production with pathogenicity of Streptomyces scabies isolates from scab infected potato tubers[J]. American Potato Journal, 1991, 68(10):675-680.
[8] Hiltunen LH, Weckman A, Ylhainen A, et al.Responses of potato cultivars to the common scab pathogens, Streptomyces scabies and S. turgidiscabies[J]. Annals of Applied Biology, 2015, 146(3):395-403.
[9] 布坎南. 伯杰. 细菌鉴定手册[M]. 北京:科学出版社, 1984.
[10] 蔡妙英, 东秀珠. 常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001.
[11] 李勇, 郭凤柳, 赵伟全, 等. 马铃薯疮痂病菌拮抗菌ZWQ-1的鉴定及防效测定[J]. 河南农业科学, 2012, 41(10):94-99.
[12] Chen S, Zhang M, Wang J, et al.Biocontrol effects of Brevibacillus laterosporus AMCC100017 on potato common scab and its impact on rhizosphere bacterial communities[J]. Biological Control, 2017, 106:89-98.
[13] Liu DQ, Anderson NA, Kinkel LL.Field evaluation of antagonistic Streptormyces strains on biocontrol of potato scab[J]. Acta Phytopa Thologica Sinica, 2000, 30(3):237-244.
[14] Hiltunen LH, Ojanpera TH, Richter E, et al.Interactions and biocontrol of pathogenic Streptomyces strains co-occurring in potato scab lesions[J]. Journal of Applied Microbiology, 2009, 106(1):199.
[15] 李勇, 郭凤柳, 赵伟全, 等. 马铃薯疮痂病菌拮抗菌ZWQ-1的鉴定及防效测定[J]. 河南农业科学, 2012, 41(10):94-99.
[16] 徐红艳, 王卫, 王彬, 等. 刺五加内生放线菌对马铃薯疮痂病菌拮抗作用初探[J]. 西北农业学报, 2016, 25(11):1742-1748.
[17] 刘利强, 杨士玲, 陈强, 等. 30亿个/g甲基营养型芽孢杆菌可湿性粉剂防治黄瓜灰霉病田间药效试验[J]. 现代农业科技, 2014(9):130-130.
[18] 谢学文, 董瑞利, 石延霞, 等. 黄瓜炭疽病拮抗细菌的筛选及其抑制效果[J]. 中国生物防治学报, 2016, 32(2):215-220.
[19] 周登博, 陈宇丰, 井涛, 等. 抗香蕉枯萎病甲基营养型芽孢杆菌的鉴定及定殖[J]. 中国农学通报, 2017(36):145-151.