Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (2): 119-125.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0836

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Endophytic Fungi Diversity in the Needles of Pinus densiflora with Sphaeropsis sapinea

XIE Xian1, LIANG Jun1,2, ZHANG Ming1, HU Rui-rui1, CHENG Yuan1, ZHANG Xing-yao1,2   

  1. 1. Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Key Laboratory of Forest Protection of National Forestry and Grassland Administration,Beijing 100091;
    2. Kunyushan Forest Ecosystem Research Station,Yantai 264100
  • Received:2019-09-09 Online:2020-02-26 Published:2020-02-23

Abstract: The objective of this work is to study the interaction between shoot blight of pine and endophytic fungi in needles at the microbial level and to analyze the community structure and diversity of endophytic fungi for providing basic data in the prevention of shoot blight of pine. High-throughput sequencing technology was used to determine the diversity and community structure of endophytic fungi in different level of blight of P. densiflora. Results demonstrated that the richness of endophytic fungi in the P. densiflora needles showed an upward trend,and the diversity index tended of decreasing first and then increasing. Ascomycota and Basidiomycota were phyla of the highest relative abundance in the asymptomatic needles,and the dominant species of endophytic fungi were Lapidomyces and Selenophoma. The disease caused the relative abundance of dominant species to decrease. In conclusion,the determination of fungi in the needles of P. densiflora clarified the diversity and community structure of endophytic fungi in the needles of P. densiflora with different levels of S. sapinea.

Key words: Pinus densiflora, Sphaeropsis sapinea, endophytic fungi, microbial diversity