Isolation,Screening and Plant Growth-promoting Potential of Nitrogen-fixing Strains from Paddy Soils

doi:10.13560/j.cnki.biotech.bull.1985.2019-1020

Abstract

Abstract: The aim of this study is to obtain efficient nitrogen-fixing strains and provide microbial resources for microbial regulations of biological nitrogen fixation in paddy fields. Four paddy soil samples with different nitrogen-fixing capability were selected to isolate cultivable nitrogen-fixing strains via serial dilution-plating method and enrichment procedure. Screening efficient nitrogen-fixing strains was performed by nifH gene PCR amplification,acetylene reduction activity and growth on N-free medium. Furthermore,the plant growth-promoting potentials of the efficient nitrogen-fixing strains were determined. A total of 14 cultivable microbial strains were obtained through isolation and purification. The presence of nifH gene was detected from genomic DNA of 7 newly isolated strains. Among them,the newly isolated strain P204,P205,P207 and P208 grew relatively fast and were in high acetylene reduction activity on N-free medium. Moreover,they had the plant growth-promoting potential,such as IAA production,phosphate solubilization and siderophore production. Thus,the newly isolated strains P204,P205,P207 and P208 represent potentially excellent bioresources for the future exploitation of functional genes and microbial inoculants.

Key words: soil, biological nitrogen fixation, plant growth-promoting bacteria, microbial resources

JIN Hai-yang, WANG Hui, ZHANG Yan-hui, HU Tian-long, LIN Zhi-bin, LIU Ben-juan, LIN Xing-wu, XIE Zu-bin. Isolation,Screening and Plant Growth-promoting Potential of Nitrogen-fixing Strains from Paddy Soils[J]. Biotechnology Bulletin, 2020, 36(6): 73-82.

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