Correlation Detween the Dynamic Changes of Morphological Structure and Molecular Weight of Coprinus comatus Extracellular Polysaccharides and Their Antioxidant Activity

doi:10.13560/j.cnki.biotech.bull.1985.2020-0471

Abstract

Abstract:

Detecting the structure of Coprinus comatus polysaccharides and exploring the relationship between structure and activity are of great significance for exploring its efficacy in depth. Three Coprinus comatus extracellular crude polysaccharides with fermentation times of 72 h,96 h and 120 h were prepared,and their monosaccharide composition were determined by the method of PMP pre-column derivatization combined with HPLC. The results showed that the extracellular polysaccharides fermented for 72 h,96 h and 120 h were all composed of D-mannose,L-rhamnose,D-glucose,D-galactose,and D-arabinose,but the relative molar ratio of each monosaccharide was different. Scanning electron microscopy and atomic force microscopy were used to analyze the morphology structure of polysaccharides,and the results showed that the plate-like structure of polysaccharides fermented 120 h was more regular and the molecules aggregation was stronger. High performance gel filtration chromatography was employed to analyze the molecular weight distribution,and the results demonstrated that the molecular weight of polysaccharides fermented for 120 h was larger and the distribution range was wider. In vitro antioxidant test results suggested that the polysaccharide fermented for 120 h displayed stronger antioxidant activity than that of polysaccharides fermented for 72 h and 96 h at the same concentration. The results indicate that the antioxidant activity of C. comatus extracellular polysaccharide may be related to its morphological structure and molecular weight distribution.

Key words: Coprinus comatus extracellular polysaccharides, monosaccharide analysis, morphological structure, molecular weight, antioxidant

WANG Shan-shan, SUN Min, WANG Yong-xia, LI Wei-dong, HAN Chun-chao. Correlation Detween the Dynamic Changes of Morphological Structure and Molecular Weight of Coprinus comatus Extracellular Polysaccharides and Their Antioxidant Activity[J]. Biotechnology Bulletin, 2021, 37(2): 129-137.

Figures/Tables 7

References 32

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单糖	回归方程	相关系数
D-甘露糖D-mannose	y=182277x-127844	R²=0.999 8
L-鼠李糖L-Rhamnose	y=155433x-101943	R²=0.999 2
D-葡萄糖D-glucose	y=98305x-22473	R²=0.999 7
D-半乳糖D-galactose	y=47785x+33756	R²=0.999 2
D-阿拉伯糖Arabinose	y=78214x-112323	R²=0.999 6

单糖	回归方程	相关系数
D-甘露糖D-mannose	y=182277x-127844	R²=0.999 8
L-鼠李糖L-Rhamnose	y=155433x-101943	R²=0.999 2
D-葡萄糖D-glucose	y=98305x-22473	R²=0.999 7
D-半乳糖D-galactose	y=47785x+33756	R²=0.999 2
D-阿拉伯糖Arabinose	y=78214x-112323	R²=0.999 6

发酵时间/h	单糖组成	摩尔比
72	甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖	0.019∶0.052∶0.137∶0.703∶0.183
96	甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖	0.013∶0.036∶0.246∶1.010∶0.166
120	甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖	0.028∶0.051∶0.183∶0.646∶0.156

发酵时间/h	单糖组成	摩尔比
72	甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖	0.019∶0.052∶0.137∶0.703∶0.183
96	甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖	0.013∶0.036∶0.246∶1.010∶0.166
120	甘露糖-鼠李糖-葡萄糖-半乳糖-阿拉伯糖	0.028∶0.051∶0.183∶0.646∶0.156