Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (6): 73-84.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1038

Previous Articles     Next Articles

Transcriptome Different Analysis of Tremella aurantialba at Mycelium and Fruiting Body Stages

CHEN Zheng-qi(), YU Jin-feng, FENG Yun-li, MA Ming, YUE Wan-song, GUO Xiang()   

  1. Kunming Edible Fungi Institute of All-China Federation of Supply and Marketing Cooperation,Kunming 650221
  • Received:2020-08-17 Online:2021-06-26 Published:2021-07-08
  • Contact: GUO Xiang E-mail:617866957@qq.com;guoxkm@yeah.net

Abstract:

To better understand the gene expression changes of mycelium and fruiting body stages in Tremella aurantialba,high-throughout sequencing were adopted to investigate differentially expressed genes by using RNA sequencing approach. Analysis revealed that 10 992 genes presented differences in gene expression,9 988 genes were up-regulated and 1 004 genes were down-regulated during the fruiting body stage. The Gene Ontology classification showed that the differentially expressed genes were mostly enriched in catalytic activity,binding,cellular process,metabolic process,cell and cell parts. KEGG pathway analysis revealed that the differential expressed genes were involved in three metabolic pathways of amino acid metabolism,carbohydrate metabolism and lipid metabolism. Most genes related to the three metabolic pathways were up-regulated,suggesting that a series of metabolic reactions and other co-regulation were needed during the development of T. aurantialba fruiting body,and these genes may play an important role in the development of T. aurantialba fruiting body. In this study,a lot of gene data were obtained by analyzing transcriptional expressions in T. aurantialba at mycelium and fruiting body stages,and may provide important basis in further revealing the developmental mechanisms and functional genes of T. aurantialba.

Key words: Tremella aurantialba, transcriptome, differentially expressed gene, GO function, pathway analysis