Advances in the Biological Modification of Galactose Oxidase

doi:10.13560/j.cnki.biotech.bull.1985.2023-0699

Abstract

Abstract:

As an important biological catalyst, galactose oxidase has the characteristics of high catalytic efficiency and environmental protection. It has been widely used in biosensors, food, medical treatment, chemical industry and other fields. However, natural galactose oxidase has some inherent defects such as poor stability, low catalytic efficiency, and high recovery cost, which greatly limit its application in food safety, biomedicine, and other fields. With the development of genetic engineering and protein engineering, galactose oxidase can be purposefully modified to improve the performance of the enzyme or to obtain recombinant enzymes with new functions. Therefore, the pursuit of galactose oxidase with important catalytic capacity and powerful functions has become a research hotspot in this field. In this paper, the molecular structure and catalytic mechanism of galactose oxidase are reviewed, and the relevant cases of biological modification of galactose oxidase are analyzed and summarized, aiming to provide new ideas and methods for galactose oxidase related researchers.

Key words: galactose oxidase, biological modification, rational design, directed evolution, immobilization

XIA Guang-li, CAO Na, SUN Hui-hui, ZHAO Ling, CAO Rong. Advances in the Biological Modification of Galactose Oxidase[J]. Biotechnology Bulletin, 2024, 40(1): 176-185.

Figures/Tables 4

Fig. 1 Catalytic reaction of galactose oxidase

Fig. 2 Crystal structures of eight reported galactose oxidases

Fig. 3 Crystal structure of galactose oxidase（Taken the galactose oxidase from F. graminearum as the template, PDB ID：1GOG） a: Front-view of 1GOG; b: Catalytic active sites of 1GOG

Fig. 4 Schematic diagram of galactose oxidase redox process

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