Biocontrol Effect of Burkholderia contaminans KRS634 on Cotton Red Rot Disease

doi:10.13560/j.cnki.biotech.bull.1985.2025-0497

Abstract

Abstract:

Objective To develop an environmentally-friendly, safe, and highly effective biocontrol agent against cotton red rot disease caused by Fusarium verticillioides. Method F. verticillioides was used as the target fungus, antagonistic strains were screened by the plate confrontation method to obtain a strong antagonistic activity strain. The strain was identified and analyzed through morphological observation, phylogenetic analysis of 16S rRNA sequences, and plant growth-promoting characteristics. Pot experiments were further carried out to determine the biocontrol and plant growth-promoting effects of the strain. The inhibitory effects of the strain on various fungi were tested by the confrontation culture method and the sealed fumigation method. Result The strain KRS634 exhibiting strong antagonistic activity against F. verticillioides was screened through plate confrontation assays, and was identified as Burkholderia contaminans based in 16S rRNA sequence analysis. KRS634 had the plant growth-promoting characteristics including phosphorus solubilization, potassium solubilization, nitrogen fixation, and siderophore production. The indoor experiments showed that KRS634 effectively reduced the occurrence of cotton red rot disease at the seedling stage. The disease plant rate of cotton red rot disease treated with KRS634 was 85.71%, and the disease index was 36.31, which was significantly lower than that of the control group. The fermentation broth of KRS634 also increased the emergence rate of cotton and promoted the growth of cotton. In addition, KRS634 had a broad-spectrum antagonistic effect on various pathogenic fungi such as Verticillium dahliae, Magnaporthe oryzae, and Colletotrichum gloeosporioides. Conclusion The strain B. contaminans KRS634 has a good application potential in the prevention and control of fungal diseases such as cotton red rot disease. It can be used as a candidate strain for the development of biocontrol agents against cotton red rot disease.

Key words: cotton red rot disease, Fusarium verticillioides, biological control, Burkholderia contaminans

WU Qiong, CHEN De-yong, ZHU He, WANG Dan, ZHANG Xiao-jun, CHEN Jie-yin. Biocontrol Effect of Burkholderia contaminans KRS634 on Cotton Red Rot Disease[J]. Biotechnology Bulletin, 2025, 41(12): 294-303.

Figures/Tables 10

References 37

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级别 Grade	分级标准 Grading standard	级值 Value
0级	无病状，健康植株	0
1级	下胚轴有少数小的病斑（褐色凹陷斑或红肿斑纹），病斑横向直径<下胚轴直径的1/4	1
2级	下胚轴病斑稍大，下胚轴直径的1/4≤病斑直径<下胚轴直径的1/2	2
3级	下胚轴病斑较大，下胚轴直径的1/2≤病斑直径<下胚轴直径的3/4，或下胚轴缢缩	3
4级	病斑直径>下胚轴直径的3/4，或整株萎蔫或死亡	4

级别 Grade	分级标准 Grading standard	级值 Value
0级	无病状，健康植株	0
1级	下胚轴有少数小的病斑（褐色凹陷斑或红肿斑纹），病斑横向直径<下胚轴直径的1/4	1
2级	下胚轴病斑稍大，下胚轴直径的1/4≤病斑直径<下胚轴直径的1/2	2
3级	下胚轴病斑较大，下胚轴直径的1/2≤病斑直径<下胚轴直径的3/4，或下胚轴缢缩	3
4级	病斑直径>下胚轴直径的3/4，或整株萎蔫或死亡	4

编号 Number	菌落直径平均值 Average colony diameter （mm）	抑菌率 Inhibition rate （%）
CK	68.03±0.52	-
KRS504	（35.90±0.97） a	（47.23±0.01） g
KRS507	（32.38±0.47） b	（52.40±0.00） f
KRS515	（31.91±0.20） b	（53.09±0.01） f
KRS516	（21.37±0.56） cde	（68.59±0.01） cde
KRS523	（20.02±0.37） ef	（70.57±0.00） bc
KRS524	（22.44±1.06） c	（67.01±0.02） e
KRS620	（17.99±0.14） g	（73.56±0.00） a
KRS625	（19.36±0.75） f	（71.54±0.01） b
KRS631	（21.71±0.79） cd	（68.09±0.01） de
KRS634	（17.83±0.21） g	（73.80±0.00） a
KRS638	（20.11±1.41） ef	（70.44±0.02） bc
KRS642	（32.86±0.95） b	（51.70±0.01） f
KRS666	（20.38±0.62） def	（70.04±0.01） bcd
KRS671	（19.98±0.93） ef	（70.63±0.02） bc
KRS684	（22.73±0.78） c	（66.59±0.01） e

编号 Number	菌落直径平均值 Average colony diameter （mm）	抑菌率 Inhibition rate （%）
CK	68.03±0.52	-
KRS504	（35.90±0.97） a	（47.23±0.01） g
KRS507	（32.38±0.47） b	（52.40±0.00） f
KRS515	（31.91±0.20） b	（53.09±0.01） f
KRS516	（21.37±0.56） cde	（68.59±0.01） cde
KRS523	（20.02±0.37） ef	（70.57±0.00） bc
KRS524	（22.44±1.06） c	（67.01±0.02） e
KRS620	（17.99±0.14） g	（73.56±0.00） a
KRS625	（19.36±0.75） f	（71.54±0.01） b
KRS631	（21.71±0.79） cd	（68.09±0.01） de
KRS634	（17.83±0.21） g	（73.80±0.00） a
KRS638	（20.11±1.41） ef	（70.44±0.02） bc
KRS642	（32.86±0.95） b	（51.70±0.01） f
KRS666	（20.38±0.62） def	（70.04±0.01） bcd
KRS671	（19.98±0.93） ef	（70.63±0.02） bc
KRS684	（22.73±0.78） c	（66.59±0.01） e