Screening of the Oxalate-degrading Lactobacillus Strains Using Protolast Fusion Technology

Abstract

Abstract: Bifidobacterium lactis with oxalate-degrading capacity can efficiently reduce the oxalate in vivo, and can be used to prevent and treat kidney stone diseases. While Bifidobacterium lactis is poorly oxygen-tolerant, that can not be employed as microbial ecological agents. In this study, protoplast fusion was studied between Bifidobacterium lactis with oxalate-degrading capacity and oxygen-tolerant lactobacillus acidophilus. Under the optimum conditions of protoplast fusion with PEG 6000 concentration 50%, fusion time 7 min, fusion temperature 30℃ , concentration of CaCl2 0.02 mol/ L and the concentration of MgCl2 0.5 mol/ L, the fusion rate reached 7.6%, and a oxygen-tolerant fusant showing the level of oxalate degradation at 13.4% was obtained.

Key words: Oxalate , Lactobacillus , Protoplast fusion , Selected , Fusion frequency

Zhang Yu, Mao Shuhong, Lu Fupin, Nie Shijian. Screening of the Oxalate-degrading Lactobacillus Strains Using Protolast Fusion Technology[J]. Biotechnology Bulletin, 2013, 0(1): 186-190.

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