Sequence Analysis of Amino Terminus of mOmpA from Antibiotic Resistant Escherichia coli

Abstract

Abstract: In the present study, we amplified the mOmpA from an isolated antibiotic resistant E. coli strain and subsequently constructed the expression vector pET32a-mOmpA. DNA sequence alignment analysis indicated that the amino terminus of the mOmpA shared 79.93% homology with the OmpA. The mOmpA amino terminus was 81.17% identical to that of the OmpA. Protein structure predication analysis through Swiss-Model suggested that the loops orientation of mOmpA N-termunus was dramatically changed compared with OmpA. Our present study promotes to better understand the structure and functions of E. coli OmpA and E. coli antibiotic resistance mechanisms.

Key words: Antibiotic resistance, Outer membrane protein, OmpA E. coli, Mutation

Zhao Zhiping, Nie Xin, Li Zaixin, Ding Jie, Zhang Zhi, Xie Wanru. Sequence Analysis of Amino Terminus of mOmpA from Antibiotic Resistant Escherichia coli[J]. Biotechnology Bulletin, 2013, 0(3): 155-159.

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