Advance and Some Strategies of Monocotyledons Transformation Mediated by Agrobacterium tumefaciens

Abstract

Abstract: In plant gene engineering, the Agrobacterium-mediated method is studied more deeply at present, and its technology is relatively perfect and effective. But the monocotyledons are not Agrobacterium natural host, so the research applications in monocot genetic transformation by Agrobacterium-mediated method are restricted. In recent years, with the rapid development of molecular biology, the transformation mechanism and influencing factors of Agrobacterium-mediated method are continuously explored and researched, making the Agrobacterium-mediated method is gradually applied in monocotyledons, especially gramineous crop, which bring breakthrough. In this paper, the Agrobacterium transformation mechanism, characteristic and factors influencing the monocotyledons conversion were introduced；The research progress in three crops genetic improvement such as wheat, maize and rice by Agrobacterium-mediated method were reviewed；The strategies improveing the efficiency of the monocotyledons transformated by Agrobacterium including ultrasonic treatment, addition of surface active agent, antioxidant and acetyl clove ketone to the culture media were summarized. It could be valuable for the further improvement and optimization of Agrobacterium-mediated monocotyledons transformation.

Key words: Agrobacterium, Monocotyledons plants, Research advances, Strategies

Zhang Jie, Zhou Yan. Advance and Some Strategies of Monocotyledons Transformation Mediated by Agrobacterium tumefaciens[J]. Biotechnology Bulletin, 2013, 0(5): 7-14.

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