Gene Editing Tools Mediated by CRISPR-Cas

Abstract

Abstract: Recently, with the deepening of the research and transformation of CRISPR-Cas system, it has been applied as a new gene targeting modification technology, which can be directed to silence target genes. At present, this technology has been successfully used in HEK293, mouse and zebrafish cells and generated stable gene knockout cell lines, meanwhile gene knockout models has been constructed on the models animals such as mice and fruit flies. With the advantages of simple design, short time-consuming and higher efficiency, CRISPR-Cas has become a gene targeting knockout technology with broad application prospect. In this paper, structure and function mechanism of the CRISPR-Cas, also the current applications of Cas9/gRNA were reviewed.

Key words: CRISPR-Cas, Genetic modification, Gene knockout

Zuo Qisheng, Li Dong, Zhang Ya’ni, Li Bichun. Gene Editing Tools Mediated by CRISPR-Cas[J]. Biotechnology Bulletin, 2014, 0(7): 37-43.

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