Quantitative Analysis on Feedback of AGPase to Photosynetic Rate in Potato

Abstract

Abstract: To reveal feedback regulation of ADP-pyrophosphrolase（AGPase）to photosynthesis, AGPase activities（AA）, starch contents（SC）in tuber, photosynthetic rates（PR）, chlorophyll contents（CC）and sucrose contents（SUC）in leafs were assayed with potato transgenic lines showing difference on ADP-pyrophosphrolase activities. The data analysis showed that AA, SC and PR in transgenic lines were significantly higher than control line. Significantly differences were found between AA, SC and PR among the lines. Correlation analysis showed that there were positive correlations between AA, SC and PR. Per unit rising of AGPase activities can contributed 2.5% increase of starch contents in sink tissue. AGPase was a factor playing a role in regulation of photosynthetic rates. The result indicated that AGPase not only regulates starch accomulation on the downstream, also feedback to modulate photosynthetic rate.

Key words: Starch contents, Photosynthetic rate , ADP-pyrophosphrolase, Correlation

Bai Hua,Cui Xueqiong,Bai Shaoxing,Yao Xinling. Quantitative Analysis on Feedback of AGPase to Photosynetic Rate in Potato[J]. Biotechnology Bulletin, 2014, 0(11): 125-129.

References

[1]K?tting O, Kossmann J, Zeeman SC, et al. Regulation of starch metabolism：the age of enlightenment?[J]. Curr Opin Plant Biol, 2010, 13（3）：321-329.
[2]Tao X, Fang Y, Xiao Y, et al. Comparative transcriptome analysis to investigate the high starch accumulation of duckweed（Landoltia punctata）under nutrient starvation[J]. Biotechnol Biofuels, 2013, 6（1）：72.
[3]Dodd AN, Kusakina J, Hall A, et al. The circadian regulation of photosynthesis[J]. Photosynth Res, 2014, 119（1-2）：181-190.
[4]Stitt M. Progress in understanding and engineering primary plant metabolism[J]. Curr Opin Biotechnol, 2013, 24（2）：229-238.
[5]Seferoglu AB, Baris I, Morgil H, et al. Transcriptional regulation of the ADP-glucose pyrophosphorylase isoforms in the leaf and the stem under long and short photoperiod in lentil[J]. Plant Sci, 2013, 205-206：29-37.
[6]Stark DM, Timmermam KP, Barry GF, et al. Regulation of the amount of starch in tissues by ADP-glucose pyrophosphorylase[J]. Science, 1992, 258（5080）：287-292.
[7]Ragel P, Streb S, Feil R, et al. Loss of starch granule initiation has a deleterious effect on the growth of Arabidopsis plants due to an accumulation of ADP-glucose[J]. Plant Physiol, 2013, 163（1）：75-85.
[8]Li N, Zhang S, Zhao Y, et al. Over-expression of AGPase genes enhances seed weight and starch content in transgenic maize[J]. Planta, 2011, 233（2）：241-250.
[9]Kang G, Liu G, Peng X, et al. Increasing the starch content and grain weight of common wheat by overexpression of the cytosolic AGPase large subunit gene[J]. Plant Physiol Biochem, 2013, 73：93-98.
[10]H?drich N, Hendriks JH, K?tting O, et al. Mutagenesis of cysteine 81 prevents dimerization of the APS1 subunit of ADP-glucose pyrophosphorylase and alters diurnal starch turnover in Arabidopsis thaliana leaves[J]. Plant J, 2012, 70（2）：231-242.
[11]Wickliff JL, Aronoff S. Quantitative measurement of leaf chlorophylls by spectrophotometry of their pheophytins in aqueous alcoholic extracts[J]. Plant Physiol, 1962, 37（5）：584-589.
[12]姚新灵, 黄婷, 马一婧, 等. 荧光发光测定AGPase活力方法初步建立及应用[J]. 西北植物学报, 2009, 29（9）：1920-1923.
[13]Geigenberger P, Kolbe A, Tiessen A. Redox regulation of carbon storage and partitioning in response to light and sugars[J]. J Exp Bot, 2005, 56（416）：1469-1479.
[14]Thorm?hlen I, Ruber J, von Roepenack-Lahaye E, et al. Inactivation of thioredoxin f1 leads to decreased light activation of ADP-glucose pyrophosphorylase and altered diurnal starch turnover in leaves of Arabidopsis plants[J]. Plant Cell Environ, 2013, 36（1）：16-29.
[15]Schmitz J, Heinrichs L, Scossa F, et al. The essential role of sugar metabolism in the acclimation response of Arabidopsis thaliana to high light intensities[J]. J Exp Bot, 2014, 65（6）：1619-1636.