Isolation，Identification and Algicidal Activity of Acinetobacter calcoaceticus

doi:10.13560/j.cnki.biotech.bull 1985.2015.04.020

Abstract

Abstract: The microcystis bloom not only result in the death of the aquatic animals due to hypoxia, but also produce mycrocystins which is harmful to the human beings and wild animals. One algicidal bacterium against toxic Microcystis aeruginosa PCC 7806 was isolated from the Baiguishan Reservoir in Pingdingshan of Henan province using liquid infection technology and was named algicidal bacterium 5. The 16S rDNA sequence analysis indicated that this bacterium belongs to Acinetobacter calcoaceticus. It specificallyremovesPCC 7806bycelllysis, but has no effect on FACHB-930 and Scenedesmus obliquus. Interestingly, the growth of haematococcus and Chlamydomonas reinhardtii are promoted. The algicidal effect of bacterium against PCC 7806 is better when the volume ratio of 1∶1. The thalli and cell-free filtrate shows the same lytic effect. There are no bacteria adhering to the surface of the PCC 7806. And no bacterial biofilm were observed. These results may suggest that The A. calcoaceticus may secrete substance and compete for nutrients to remove PCC 7806.

Key words: algicidal bacterium, Acinetobacter calcoaceticus, 16S rDNA, Microcystis aeruginosa, algicidal characteristics

Wang Yun, Zhang Yemeng, Li Peipei. Isolation，Identification and Algicidal Activity of Acinetobacter calcoaceticus[J]. Biotechnology Bulletin, 2015, 31(3): 140-145.

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