Screening and Identification of Maize Growth-promoting Rhizobacteria and Its Promoting Effects on Maize

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.025

Abstract

Abstract: Five maize plant growth-promoting rhizobacteria（PGPR）strains designated as YM1,YM2,YM3,YM4,and YM5 respectively were isolated and purified from the sandy soil. Based on their nitrogenase activity,ability of dissolving phosphorus and potassium,and indole acetic acid（IAA）synthetic quantity,the active microbial inoculum was screened for producing efficient microbial fertilizer. The results of shake flask culture indicated that strain YM4 was observed to have a stronger ability of dissolving phosphorus and potassium,and higher nitrogenase activity accompanied with the strongest synthetic quantity of IAA than other strains. Its nitrogenase activity reached 15.53 nmol C₂H₄/（h·mL）,the transformation amounts of tricalcium phosphate and potassium silicate were 128.90 mg/L,17.40 mg/L respectively,and the synthetic quantity of IAA was 37.18 mg/L. YM4 was identified as Bacillus pumilus based on morphological observation,physiological and biochemical characteristics test and the conserved sequence analysis of 16S rDNA. Compared to the control treatment,pot experiment results suggested that the concentrations of IAA,available nitrogen,phosphorous,and potassium in the potting soil inoculated with YM4 significantly increased by 136.36%,42.1%,67.41%,and 14.29%,respectively;moreover,the morphologies of plant roots changed significantly,i.e.,the root length,surface area,root volumes and root tips of maize increased by 172.24%,141.73%,112.14%,and 104.53%,respectively;additionally,the average wet weight,the height of maize,SPAD value increased by 130.07%,150.65%,and 151.56% and the total N,P,K content of maize significantly increased by 120.99%,166.33% and 138.21% respectively. We may conclude that YM4 possess the abilities of efficiently fixing nitrogen,solubilizing phosphorus and potassium,and synthetizing IAA,thus has great application potential in the development and utilization of agricultural production.

Key words: plant growth-promoting rhizobacteria, nitrogen fixation, phosphate-solubilizing activity, potassium-solubilizing activity, IAA

WAN Bing-bing, LIU Ye, WU Yue, ZHANG Dong-yan, WANG Guo-wen, JIANG Ying. Screening and Identification of Maize Growth-promoting Rhizobacteria and Its Promoting Effects on Maize[J]. Biotechnology Bulletin, 2016, 32(8): 169-176.

References

[1] 孙本喆, 郭新平, 曾苏明, 等. 我国玉米生产现状及发展对策[J]. 玉米学, 2003, S2：32-33.
[2] 石德权, 郭庆法, 汪黎明等. 我国玉米品质现状、问题及发展优质食用玉米对策[J]. 玉米科学, 2001, 9（2）：3-7.
[3] 荣良燕, 姚拓, 黄高宝, 等. 植物根际优良促生菌（PGPR）筛选及其接种剂部分替代化肥对玉米生长影响研究[J]. 干旱地区农业研究, 2013, 31（2）：59-65.
[4] Prasad R, Kumar M, Varma A. Role of PGPR in Soil Fertility and Plant Health[M]// Plant-Growth-Promoting Rhizobacteria（PGPR）and Medicinal Plants. Springer International Publishing, 2015：247-260.
[5] Zhou T, Yang Y, Jin Y. Study on preparation of chelated micro-fertilizer by oxidation of enzymatic hydrolysis residues of corn stalk[J]. Journal of Cellulose Science and Technology, 2012, 20 （1）： 16-25.
[6] 张瑞福, 颜春荣, 张楠, 等. 微生物肥料研究及其在耕地质量提升中的应用前景[J]. 中国农业科技导报, 2013, 5：8-16.
[7] Adesemoye AO, Torbert HA, Kloepper JW. Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. [J]. Microbial Ecology, 2009, 58（4）：921-929.
[8] Adesemoye AO, Torbert HA, Kloepper JW. Increased plant uptake of nitrogen from 15 N-depleted fertilizer using plant growth-promoting rhizobacteria[J]. Applied Soil Ecology, 2010, 46（1）：54-58.
[9] Kloepper JW, Schroth MN. Plant growth-promoting rhizobacteria on radishes[C]. Proceedings of the 4th international conference on plant pathogenic bacteria, 1978, 2：879-882.
[10] 王素英, 陶光灿, 谢光辉, 等. 我国微生物肥料的应用研究进展[J]. 中国农业大学学报, 2003, 8（1）：14-18.
[11] 鲍士旦. 土壤农化分析[M]. 第3版. 北京：中国农业出版社, 2000.
[12] 廖婷婷, 翟磊, 高成华, 等. 一株产甘露聚糖酶菌株的分离鉴定及酶的纯化与性质[J]. 微生物学报, 2011, 51（11）：1520-1526.
[13] Libbert E, Risch H. Interactions between plants and epiphytic bacteria regarding their auxin metabolism. V. Isolation and identification of the IAA-producing and destroying bacteria from pea plants[J]. Physiol Plantarum, 1969, 22：51-58.
[14] Glickmann E, Dessaux Y. A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria[J]. Applied & Environmental Microbiology, 1995, 61（2）：793-796.
[15] 王补全. 玉米根际促生菌的开发研究[D]. 太原：山西大学, 2010.
[16] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京：科学出版社, 2001.
[17] R·E·布坎南, N·E·吉本斯. 伯杰细菌鉴定手册[M]. 北京：科学出版社, 1984.
[18] 夏北成. 分子生物学方法在微生物生态学中的应用[J]. 中山大学学报：自然科学版, 1998, 2：98-102.
[19] Abed ZA, Abed RT. Determining genetic distance by RAPD-PCR of maize inbred lines produced by reciprocal recurrent selection[J]. Journal of Agricultural Technology, 2013, 9（7）：1799-1807.
[20] 陈中义, 张杰, 黄大昉, 等. 植物病害生防芽孢杆菌抗菌机制与遗传改良研究[J]. 植物病理学报, 2003, 33（2）：97-103.
[21] Adesemoye A, Torbert H, Kloepper J. Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system[J]. Canadian Journal of Microbiology, 2008, 54（10）：876-886.
[22] Nosheen A, Bano A. Potential of plant growth promoting rhizobacteria and chemical fertilizers on soil enzymes and plant growth[J]. Pakistan Journal of Botany, 2014, 46（46）：1521-1530.
[23] Habibi S, Djedidi S, Prongjunthuek K, et al. Physiological and genetic characterization of rice nitrogen fixer PGPR isolated from rhizosphere soils of different crops[J]. Plant & Soil, 2014, 379：1-16.
[24] Shrestha A, Kim BS, Park DH. Biological control of bacterial spot disease and plant growth-promoting effects of lactic acid bacteria on pepper[J]. Biocontrol Science & Technology, 2014, 24（7）：763-779.
[25] Yadav J, Verma JP. Effect of seed inoculation with indigenous Rhizobium and plant growth promoting rhizobacteria on nutrients uptake and yields of chickpea（Cicer arietinum L. ）[J]. European Journal of Soil Biology, 2014, 63（4）：70-77.
[26] 赵青云, 赵秋芳, 王辉, 等. 根际促生菌Bacillus subtilisY-IVI在香草兰上的应用效果研究[J]. 植物营养与肥料学报, 2015, 21（2）：535-540.
[27] Yin X, McClure MA, Jaja N, et al. In-season prediction of corn yield using plant height under major production systems[J]. Agronomy Journal, 2011, 103（3）：923-929.
[28] Eleanor E, Campbell, Jane MF, et al. Assessing the soil carbon, biomass production, and nitrous oxide emission impact of corn stover management for bioenergy feedstock production using DAYCENT[J]. Bioenergy Research, 2014, 7（2）：491-502.
[29] Umesh MR, Manjunatha N, Shankar MA, et al. Influence of nutrient supply levels on yield, nutrient uptake, grain quality and economics of corn（Zea mays L. ）in Alfisols of Karnataka[J]. Indian Journal of Dryland Agricultural Research and Development, 2014, 29（1）：73-78.
[30] Radhakrishnan R, Shim KB, Lee BW, et al. IAA-producing Penicillium sp. NICS01 triggers plant growth and suppresses Fusarium sp. -induced oxidative stress in sesame（Sesamum indicum L. ）[J]. J Microbiol Biotechnol, 2013, 23：856-863.
[31] Li G, Yang FT, Jiang XL, et al. Effects of plow pan on SPAD value and chloroplast ultrastructure in leaves of spring maize[J]. Phyton（Buenos Aires）, 2013, 82：243-247.
[32] 童淑媛, 宋凤斌, 徐洪文. 玉米不同叶位叶片SPAD值的变化及其与生物量的相关性[J]. 核农学报, 2008, 6：869-874.