Research Progress on Exonuclease III-assisted Functional Nucleic Acid Biosensors

doi:10.13560/j.cnki.biotech.bull.1985.2018-0278

Abstract

Abstract:

Exonuclease III（Exo III）is a kind of nuclease that can specifically recognize dsDNA and catalyze the release of mononucleotides by stepwise hydrolysis from 3'-hydroxyl termini of dsDNA strands;while hydrolysis on ssDNA is limited. Based on its exonuclease activity,Exo III has been successfully employed for amplified detection of DNAs,small molecules and metal ions while combined with a series of signal outputs. Here we mainly give a detailed classifying description of the Exo III-assisted biosensors based on various target substances,and also expound the working principles and sensitivity of each sensor. In addition,we discuss the recent progresses on the integration of Exo III-assisted target-recycling strategy with other signal enhancement strategies or emerging nanomaterials in detail as well. Finally,we summarize the advantages and disadvantages of the Exo III-assisted biosensors and prospect the future development,which will be conducive to further application and continuous development of Exo III-assisted biosensors in environmental management,biological analysis and clinical diagnosis.

Key words: exonuclease III, functional nucleic acid, signal amplification, biosensor

SONG Huan, LUO Yun-bo, XU Wen-tao. Research Progress on Exonuclease III-assisted Functional Nucleic Acid Biosensors[J]. Biotechnology Bulletin, 2018, 34(9): 55-69.

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