Isolation of Efficient Nitrogen-fixing Bacteria from the Rhizosphere of Sophora flavescens and the Growth-promoting Effect of Compound Microbial Fertilizer on Seedlings

doi:10.13560/j.cnki.biotech.bull.1985.2020-0379

Abstract

Abstract: This work aims to obtain plant growth promoting rhizobacteria（PGPR）and clarify its growth-promoting characteristics,so as to promote the application of compound microbial fertilizer in matrix for Sophora flavescens seedlings. Serial dilution-plating method was used to isolate highly efficient rhizosphere nitrogen-fixing bacteria from the rhizosphere soil of three-year-old Sophora flavescens. The isolated bacteria were identified by morphological characteristics,physiological and biochemical characteristics and 16S rRNA gene sequence. Their growth-promoting ability of IAA production and phosphate-solubilizing,and nitrogenase activity were determined for screening fine strains to study their growth-promoting effects on potted S. flavescens seedlings. As a result,20 strains of S. flavescens PGPR were isolated and identified as Pseudomonas,Bacillus,Ensifer,Arthrobacter and Staphylococcus epidermidis;among them,10 strains were Pseudomonas and 4 strains were Bacillus. Bacillus strain NF2-4 had the strongest IAA production of 451 mg/L and phosphate-solubilizing capacity of 1.908 mg/L,the nitrogenase activity was 1.24 nmol/（g·h）,and thus NF2-4 and Pseudomonas sp. NF1-17 with the stronger growth promotion were used as the compound microbial fertilizer for pot experiment. The results showed that the growth promotion ability to S. flavescens seedlings was compound microbial fertilizer > microbial fertilizer NF2-4 > microbial fertilizer NF1-17 > control group. The compound microbial fertilizer demonstrated the best growth promoting effect on S. flavescens seedling.

Key words: soil, biological nitrogen fixation, plant growth-promoting rhizobacteria, compound microbial fertilizer

LEI Hai-ying, ZHAO Qing-song, YANG Xiao, WANG Mao-mao, BAI Jie, SUN Yong-qi, WANG Zhi-jun. Isolation of Efficient Nitrogen-fixing Bacteria from the Rhizosphere of Sophora flavescens and the Growth-promoting Effect of Compound Microbial Fertilizer on Seedlings[J]. Biotechnology Bulletin, 2020, 36(9): 157-166.

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