Review on the Synergistic Insect-resistant Application of RNAi and Bt-transgenic Technologies

doi:10.13560/j.cnki.biotech.bull.1985.2021-0244

Abstract

Abstract:

The cultivation of transgenic insect-resistance crops has decreased the damage of insects on plants and the application of chemical pesticides in the field. However,the long-term continuous cultivation of Bt-transgenic crops worldwide has increased the resistance evolution of target insects to Bt-transgenic plants in the field,leading its long-term insect resistance to be in challenge. To delay the resistance evolution of target insects to Bt proteins and to improve the insect-resistance of transgenic crops,RNAi and Bt-transgenic biotechnologies have been synergistically applied to control agricultural pests. Here,we review the progresses on RNAi technology applied in agricultural pest-control and on the synergistic application of Bt-transgene technology in agricultural pest-control,and discuss the challenges and technological difficulties,aiming to promote the combined application of RNAi and transgenic technologies to be environment-friendly and sustainable in controlling agricultural pests.

Key words: dsRNA, RNA interference, genetically modified crops, pest control

DENG Pu-rong, LIU Yong-bo. Review on the Synergistic Insect-resistant Application of RNAi and Bt-transgenic Technologies[J]. Biotechnology Bulletin, 2021, 37(10): 216-224.

Figures/Tables 2

References 85

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物种 Species	目标基因 Target gene	Bt蛋白基因 Bt gene	联合作效率 Synergistic efficiency	参考文献Reference
马铃薯甲虫 Leptinotarsa decemlineata	prohibitin-1	Cry3Aa	死亡 Mortality	[56]
海灰翅夜蛾 Spodoptera littoralis	Sl102	Cry1Ca	免疫力下降 Down-regulation of immunocompetence	[57]
亚洲玉米螟 Ostrinia furnacalis	chymotrypsin-like genes	Cry1Ab	死亡率显著升高 Significantly increased mortalities	[60]
甜菜夜蛾 Spodoptera exigua	CHS-B	Cry1Ac 和Cry1Ca	死亡 Mortality	[61]
棉铃虫 Helicoverpa armigera	JHAMT和JHBP	Cry1Ac	死亡 Mortality	[6]
棉铃虫 Helicoverpa armigera	v-ATPase-A	Cry1Ac	体重抑制率显著升高,对Cry1Ac更敏感 Higher weight inhibition,more sensitive to Cry1Ac	[62]

物种 Species	目标基因 Target gene	Bt蛋白基因 Bt gene	联合作效率 Synergistic efficiency	参考文献Reference
马铃薯甲虫 Leptinotarsa decemlineata	prohibitin-1	Cry3Aa	死亡 Mortality	[56]
海灰翅夜蛾 Spodoptera littoralis	Sl102	Cry1Ca	免疫力下降 Down-regulation of immunocompetence	[57]
亚洲玉米螟 Ostrinia furnacalis	chymotrypsin-like genes	Cry1Ab	死亡率显著升高 Significantly increased mortalities	[60]
甜菜夜蛾 Spodoptera exigua	CHS-B	Cry1Ac 和Cry1Ca	死亡 Mortality	[61]
棉铃虫 Helicoverpa armigera	JHAMT和JHBP	Cry1Ac	死亡 Mortality	[6]
棉铃虫 Helicoverpa armigera	v-ATPase-A	Cry1Ac	体重抑制率显著升高,对Cry1Ac更敏感 Higher weight inhibition,more sensitive to Cry1Ac	[62]

干扰途径 RNAi approach	植物 Plant	昆虫 Insect	目标基因 Target gene	参考文献 Reference
宿主诱导的基因沉默 HIGS	玉米和大豆 Zea mays and Glycine max	绿盲蝽 Apolygus lucorum	V-ATPase-E	[39]
	本氏烟 Nicotiana benthamiana	桃蚜 Myzus persicae	MpC002 and Rack-1	[36]
	拟南芥 Arabidopsis thaliana	桃蚜 Myzus persicae	MySP	[44]
	烟草 Nicotiana tabacum	烟粉虱 Bemisia tabaci	acetylcholinesterase（AChE） ecdysone receptor（EcR）	[37]
	小麦 Triticum aestivum	麦长管蚜 Sitobion avenae	zinc finger（SaZFP）	[40]
	马铃薯 Solanum tuberosum	马铃薯甲虫 Leptinotarsa decemlineata	EcR	[38]
	拟南芥 Arabidopsis thaliana 烟草 Nicotiana tabacum	棉铃虫 Helicoverpa armigera	CYP6AE14	[41]
	拟南芥 Arabidopsis thaliana	棉铃虫 Helicoverpa armigera	mitochondrial complex I（NDUFV2）	[19]
饲喂法 Dietary feeding		豌豆蚜 Acyrthosiphon pisum	ApAQP1	[76]
显微注射法 Microinjection		黄曲条跳甲 Phyllotreta striolata	odorant receptor（PsOr1）	[77]
		德国小蠊 Blattella germanica	RXR	[22]
微生物介导 Bacterial expression and oral delivery		粘虫 Mythimna separata	MseChi	[15]
		亚洲玉米螟 Ostrinia furnacalis	Glutathione-S-Transferase	[78]

干扰途径 RNAi approach	植物 Plant	昆虫 Insect	目标基因 Target gene	参考文献 Reference
宿主诱导的基因沉默 HIGS	玉米和大豆 Zea mays and Glycine max	绿盲蝽 Apolygus lucorum	V-ATPase-E	[39]
	本氏烟 Nicotiana benthamiana	桃蚜 Myzus persicae	MpC002 and Rack-1	[36]
	拟南芥 Arabidopsis thaliana	桃蚜 Myzus persicae	MySP	[44]
	烟草 Nicotiana tabacum	烟粉虱 Bemisia tabaci	acetylcholinesterase（AChE） ecdysone receptor（EcR）	[37]
	小麦 Triticum aestivum	麦长管蚜 Sitobion avenae	zinc finger（SaZFP）	[40]
	马铃薯 Solanum tuberosum	马铃薯甲虫 Leptinotarsa decemlineata	EcR	[38]
	拟南芥 Arabidopsis thaliana 烟草 Nicotiana tabacum	棉铃虫 Helicoverpa armigera	CYP6AE14	[41]
	拟南芥 Arabidopsis thaliana	棉铃虫 Helicoverpa armigera	mitochondrial complex I（NDUFV2）	[19]
饲喂法 Dietary feeding		豌豆蚜 Acyrthosiphon pisum	ApAQP1	[76]
显微注射法 Microinjection		黄曲条跳甲 Phyllotreta striolata	odorant receptor（PsOr1）	[77]
		德国小蠊 Blattella germanica	RXR	[22]
微生物介导 Bacterial expression and oral delivery		粘虫 Mythimna separata	MseChi	[15]
		亚洲玉米螟 Ostrinia furnacalis	Glutathione-S-Transferase	[78]