Community Characteristics of Methanogens in Alpine Wetland Driven by Precipitation Changes

doi:10.13560/j.cnki.biotech.bull.1985.2020-1580

Abstract

Abstract:

Soil microorganism in alpine wetland is very sensitive to climate change. In order to study the response of soil methanogens community to precipitation changes in alpine wetlands on the Qinghai-Tibet Plateau,5 precipitation gradients were set up,including natural rainfall,25% and 50% decrease of natural rainfall,and 25% and 50% increase of natural rainfall. McrA gene with high-throughput sequencing was used to analyze the community structure and diversity of soil methanogens. The dominant methanogens in alpine wetlands were Methanomicrobiales,Methanosarcinales and Methanobacteriales. The relative abundance and diversity index of methanogens significantly decreased under rainfall increasing treatment,and the relative abundance and diversity index of methanogens significantly increased under rainfall decreasing treatment. The response of methanogen community composition to 50% increase or decrease of rainfall was more significant,only Methanothrix was more sensitive to 25% rainfall increase and Methanobacterium was more sensitive to 25% rainfall reduction treatment. The richness index of methanogen community was more sensitive to the 50% increase and 25% decrease of rainfall,Shannon and Simpson diversity indices showed the opposite. Lefse analysis showed that 16 different microbial communities were generated after treatment with different precipitation gradients,with the most different microbial communities under treatment with 50% decrease of rainfall,in a total of 9 different microbial communities from the class level to the genus level. In conclusion,precipitation change significantly affects the community structure and diversity of methanogens in alpine wetland.

Key words: alpine wetland, precipitation change, methanogens, community structure, climate change

ZHANG Ni, BAO Han, ZUO Di-zhao, CHEN Ke-long. Community Characteristics of Methanogens in Alpine Wetland Driven by Precipitation Changes[J]. Biotechnology Bulletin, 2021, 37(11): 276-284.

Figures/Tables 8

Fig.1 A device to reduce rainfall

Table 1 Sample data and OTU statistics

ID	Clean_tags	Effective_tags	Singleton	Singleton/%	Chimeras	Chimeras/%	OTUs
WJa	108 821	99 419	8 559	7.87	843	0.78	868
WJb	100 981	91 721	8 425	8.29	835	0.81	859
WZa	107 493	103 220	4 212	3.91	61	0.06	663
WZb	99 147	94 911	4 180	4.22	57	0.06	615
Wck	111 505	104 924	6 159	5.49	422	0.37	763

Fig.2 Species accumulation graph

Fig.3 Venn diagram of soil samples under different treatments

Fig.4 Column diagram of soil Methanogenium community

Fig.5 α diversity index of methanogen community in alpine wetland

Fig.6 PCoA analysis of soil samples in different groups of alpine wetland（Weight_UniFrac）

Fig.7 Lefse analysis of methanogenic bacteria in alpine wetland soil

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