Research Progress on Microbial Degradation of Thiocyanate in Wastewater

doi:10.13560/j.cnki.biotech.bull.1985.2020-0661

Abstract

Abstract:

Thiocyanate（SCN^-）is a common pollutant in gold mine,textile,dyeing and coking industries,is toxic and harmful to biological safety. At present,with the development of modern biotechnology,the community structure,genetic and metabolic diversity of thiocyanate degradation by microorganisms have been clarified through high-throughput sequencing,transcriptome sequencing,DNA fingerprinting and targeted gene amplification,which indicates that thiocyanate degradation by microorganisms is the most feasible approach for remediation. In this paper,the types of thiocyanate-degrading microorganisms,the influence of carbon,sulfur and nitrogen cycle,the mechanism of thiocyanate degradation are reviewed,and the significance and issues of thiocyanate degradation by microorganisms are discussed,aiming to provide theoretical support for the treatment of thiocyanate in wastewater.

Key words: thiocyanate, microbial degradation, wastewater

XIAO Xiao-shuang, AN Xue-jiao, YE Han-yuan, WANG Lin-ping, ZHONG Bin, ZHANG Qing-hua. Research Progress on Microbial Degradation of Thiocyanate in Wastewater[J]. Biotechnology Bulletin, 2021, 37(2): 224-235.

Figures/Tables 4

References 81

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分类依据	菌株类型	参考文献
自养菌	Thiobacillus thioparus、Paracoccus spp.、Thiobacillus denitrificans、Thiohalophilus thiocyanoxidans、Halothiobacillus spp.、Thioalkalivibrio paradoxus、Thioalkalivibrio thiocyanoxidans、Thioalkalivibrio thiocyanodenitrificans、T. denitrificans、T. thiocyanodenitrificans and Pseudomonas aeuginosa	[9-10,27-42]
异养菌	Ralstonia、Sphingomonas、Klebsiella、Pseudomonas、Arthrobacter and Methylobacterium	[12,43-47,49]
混合营养菌	Burkholderia phytofirmans	[50]
真菌	Acremonium strictum、Schizophyllum commune、Polyporus arcularius、Ganoderma applanatum、Pleurotus eryngii、Clavariadelphus truncatus、Cerrena unicolor、Trametes versicolor、Ganoderma lucidum and Schizophyllum commune	[51-52]

分类依据	菌株类型	参考文献
自养菌	Thiobacillus thioparus、Paracoccus spp.、Thiobacillus denitrificans、Thiohalophilus thiocyanoxidans、Halothiobacillus spp.、Thioalkalivibrio paradoxus、Thioalkalivibrio thiocyanoxidans、Thioalkalivibrio thiocyanodenitrificans、T. denitrificans、T. thiocyanodenitrificans and Pseudomonas aeuginosa	[9-10,27-42]
异养菌	Ralstonia、Sphingomonas、Klebsiella、Pseudomonas、Arthrobacter and Methylobacterium	[12,43-47,49]
混合营养菌	Burkholderia phytofirmans	[50]
真菌	Acremonium strictum、Schizophyllum commune、Polyporus arcularius、Ganoderma applanatum、Pleurotus eryngii、Clavariadelphus truncatus、Cerrena unicolor、Trametes versicolor、Ganoderma lucidum and Schizophyllum commune	[51-52]

影响因素	影响原因	参考文献
温度	温度的降低和升高会影响微生物的生长、代谢和繁殖以及SCNase的活性	[29,72,76]
pH	pH的过低和过高会影响微生物的生长、代谢和繁殖以及SCNase的活性	[9,29,36,72,76,80-81]
溶解氧	好氧微生物需要高浓度的溶解氧,厌氧微生物则需要低浓度的溶解氧	[28-29]
硫氰酸盐浓度	硫氰酸盐浓度过高会出现底物抑制	[9-11,28,76,80-81]
无机盐	无机盐是微生物生长、代谢和繁殖过程所必需的	[3,76]
外加营养物质（C源或N源）	有些微生物单一利用硫氰酸盐中的碳源或者氮源,这就需要相对应的补充氮源或碳源	[9]
代谢产物浓度	产物浓度过高会抑制微生物降解硫氰酸盐	[9-10,28-29,76,80]

影响因素	影响原因	参考文献
温度	温度的降低和升高会影响微生物的生长、代谢和繁殖以及SCNase的活性	[29,72,76]
pH	pH的过低和过高会影响微生物的生长、代谢和繁殖以及SCNase的活性	[9,29,36,72,76,80-81]
溶解氧	好氧微生物需要高浓度的溶解氧,厌氧微生物则需要低浓度的溶解氧	[28-29]
硫氰酸盐浓度	硫氰酸盐浓度过高会出现底物抑制	[9-11,28,76,80-81]
无机盐	无机盐是微生物生长、代谢和繁殖过程所必需的	[3,76]
外加营养物质（C源或N源）	有些微生物单一利用硫氰酸盐中的碳源或者氮源,这就需要相对应的补充氮源或碳源	[9]
代谢产物浓度	产物浓度过高会抑制微生物降解硫氰酸盐	[9-10,28-29,76,80]