Physiological Response of Hibiscus syriacus Varieties to Cadmium Stress and Evaluation of Cadmium Tolerance

doi:10.13560/j.cnki.biotech.bull.1985.2021-0132

Abstract

Abstract:

The objective is to study the physiological effects of cadmium stress on Hibiscus syriacus and compare the cadmium tolerance of H. syriacus varieties,so as to provide basis for the selection of plant materials in cadmium polluted areas. Three H. syriacus cultivars were used as materials,and pot experiment was conducted to determine their growth and physiological indexes. Membership function analysis was used to comprehensively compare the cadmium tolerance of varieties. The growth of height and ground diameter,the content of chlorophyll A+B and soluble protein of H. syriacus first increased and then decreased or gradually decreased with the increase of stress concentration,and the degree of decrease gradually increased with the extension of time. Free proline,malondialdehyde content and SOD activity increased gradually with the increase of stress concentration and time,but decreased in different degrees under long-term high concentration stress. The results of membership function analysis showed that the tolerance of varieties varied with the stress concentration. The growth of H. syriacus under Cd stress was promoted by low concentration and inhibited by high concentration,and with the extension of time,the promotion weakened and the inhibition strengthened. In addition,the performance of ‘Hong xing’ is the best,which is worth of promoting.

Key words: Hibiscus syriacus, cadmium tolerance, cadmium stress, physiological response, membership function, varieties

YANG Fu-rong, WANG Xiao-hong, XIAO Qi, FANG Juan, LI Li-hua. Physiological Response of Hibiscus syriacus Varieties to Cadmium Stress and Evaluation of Cadmium Tolerance[J]. Biotechnology Bulletin, 2022, 38(1): 98-107.

Figures/Tables 7

References 32

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品种 Varieties	镉处理浓度 Cadmium treatment concentration/（mg·kg^-1）	株高增长量 Growth of plant height/cm	地径增长量 Growth of the earth’s path/cm
‘牡丹’ ‘f. paeoniflorus’	0	35.633±23.981a	0.226±0.212a
	50	18.267±3.19ab	0.316±0.087a
	100	20.667±12.254ab	0.236±0.109a
	200	7.667±1.704b	0.131±0.087a
	400	5.6±1.803b	0.101±0.052a
‘红星’ ‘Hong xing’	0	17.833±5.829ab	0.224±0.137a
	50	33.533±14.994a	0.221±0.114a
	100	21.567±13.428ab	0.203±0.063a
	200	22.8±3.951ab	0.161±0.077a
	400	6.667±3.63b	0.101±0.096a
‘白花重瓣’ ‘f. albus-plenus’	0	13.067±9.028a	0.26±0.158a
	50	22.4±14.245a	0.217±0.151a
	100	10.2±1.97a	0.12±0.048a
	200	8.033±6.463a	0.097±0.044a
	400	7.2±5.012a	0.094±0.019a

品种 Varieties	镉处理浓度 Cadmium treatment concentration/（mg·kg^-1）	株高增长量 Growth of plant height/cm	地径增长量 Growth of the earth’s path/cm
‘牡丹’ ‘f. paeoniflorus’	0	35.633±23.981a	0.226±0.212a
	50	18.267±3.19ab	0.316±0.087a
	100	20.667±12.254ab	0.236±0.109a
	200	7.667±1.704b	0.131±0.087a
	400	5.6±1.803b	0.101±0.052a
‘红星’ ‘Hong xing’	0	17.833±5.829ab	0.224±0.137a
	50	33.533±14.994a	0.221±0.114a
	100	21.567±13.428ab	0.203±0.063a
	200	22.8±3.951ab	0.161±0.077a
	400	6.667±3.63b	0.101±0.096a
‘白花重瓣’ ‘f. albus-plenus’	0	13.067±9.028a	0.26±0.158a
	50	22.4±14.245a	0.217±0.151a
	100	10.2±1.97a	0.12±0.048a
	200	8.033±6.463a	0.097±0.044a
	400	7.2±5.012a	0.094±0.019a

Cd处理浓度Cadmium concentration/（mg·kg^-1）	品种 Varieties	隶属函数值 Membership function value							平均值 Average value	排名 Ranking
		X1	X2	X3	X4	X5	X6	X7
50	‘牡丹’（‘f. paeoniflorus’）	0.368	0.417	0.392	0.539	0.556	0.367	0.481	0.446	3
	‘红星’（‘Hong xing’）	0.532	0.634	0.461	0.642	0.500	0.371	0.409	0.507	1
	‘白花重瓣’（‘f. albus-plenus’）	0.578	0.353	0.560	0.355	0.667	0.422	0.518	0.493	2
100	‘牡丹’（‘f. paeoniflorus’）	0.560	0.454	0.557	0.475	0.667	0.410	0.520	0.520	3
	‘红星’（‘Hong xing’）	0.460	0.559	0.646	0.646	0.567	0.639	0.529	0.578	1
	‘白花重瓣’（‘f. albus-plenus’）	0.421	0.403	0.637	0.477	0.630	0.599	0.515	0.526	2
200	‘牡丹’（‘f. paeoniflorus’）	0.480	0.479	0.420	0.381	0.500	0.600	0.426	0.469	2
	‘红星’（‘Hong xing’）	0.608	0.544	0.603	0.658	0.400	0.465	0.467	0.535	1
	‘白花重瓣’（‘f. albus-plenus’）	0.399	0.462	0.427	0.400	0.452	0.399	0.537	0.439	3
400	‘牡丹’（‘f. paeoniflorus’）	0.429	0.427	0.384	0.548	0.524	0.353	0.521	0.455	3
	‘红星’（‘Hong xing’）	0.463	0.560	0.337	0.394	0.422	0.495	0.602	0.468	2
	‘白花重瓣’（‘f. albus-plenus）’	0.480	0.526	0.558	0.502	0.377	0.592	0.500	0.505	1

Cd处理浓度Cadmium concentration/（mg·kg^-1）	品种 Varieties	隶属函数值 Membership function value							平均值 Average value	排名 Ranking
		X1	X2	X3	X4	X5	X6	X7
50	‘牡丹’（‘f. paeoniflorus’）	0.368	0.417	0.392	0.539	0.556	0.367	0.481	0.446	3
	‘红星’（‘Hong xing’）	0.532	0.634	0.461	0.642	0.500	0.371	0.409	0.507	1
	‘白花重瓣’（‘f. albus-plenus’）	0.578	0.353	0.560	0.355	0.667	0.422	0.518	0.493	2
100	‘牡丹’（‘f. paeoniflorus’）	0.560	0.454	0.557	0.475	0.667	0.410	0.520	0.520	3
	‘红星’（‘Hong xing’）	0.460	0.559	0.646	0.646	0.567	0.639	0.529	0.578	1
	‘白花重瓣’（‘f. albus-plenus’）	0.421	0.403	0.637	0.477	0.630	0.599	0.515	0.526	2
200	‘牡丹’（‘f. paeoniflorus’）	0.480	0.479	0.420	0.381	0.500	0.600	0.426	0.469	2
	‘红星’（‘Hong xing’）	0.608	0.544	0.603	0.658	0.400	0.465	0.467	0.535	1
	‘白花重瓣’（‘f. albus-plenus’）	0.399	0.462	0.427	0.400	0.452	0.399	0.537	0.439	3
400	‘牡丹’（‘f. paeoniflorus’）	0.429	0.427	0.384	0.548	0.524	0.353	0.521	0.455	3
	‘红星’（‘Hong xing’）	0.463	0.560	0.337	0.394	0.422	0.495	0.602	0.468	2
	‘白花重瓣’（‘f. albus-plenus）’	0.480	0.526	0.558	0.502	0.377	0.592	0.500	0.505	1