Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (5): 136-148.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0998
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ZHANG Hao(), LI Zhe(), GUO Kai, HUANG Yan-hua, HAO Yong-ren
Received:
2021-08-05
Online:
2022-05-26
Published:
2022-06-10
Contact:
LI Zhe
E-mail:arthur02@163.com;zhel@sdas.org
ZHANG Hao, LI Zhe, GUO Kai, HUANG Yan-hua, HAO Yong-ren. Functional Analysis of TvGCN5 Gene Encoding Histone Acetylase from Trichoderma viride Tv-1511[J]. Biotechnology Bulletin, 2022, 38(5): 136-148.
Fig. 1 Construction of knockout fragment and overexpression vector of TvGCN5 gene A,B:Fragment construction of knockout TvGCN5 gene. C,D:Construction of overexpression vector of TvGCN5 gene
Fig.2 Identification of overexpressing or deleting TvGCN5 gene in Trichoderma engineering strains by fluores-cence quantitative PCR and Western blot A:TvGCN5 gene transcription level in Trichoderma engineered strains by fluorescence quantitative PCR. B,C:Protein expression level of TvGCN5 in Trichoderma engineered strains by Western blot
Fig.3 Changes in the levels of histone acetylation in T. viride Tv-1511 and its engineered strains A:Quantitative detection of histone H3 acetylation level in T. viride Tv-1511 and its engineered strains by assay kit. B,C:Detection of histone H3 acetylation level in T. viride Tv-1511and its engineered strains by Western blot
Fig.4 Analysis of the salt tolerances of T. viride Tv-1511 original strain and its engineered strains A,B:Petri dish test of the salt tolerance of Trichoderma and quantitative statistics of colony diameter. C:Dry weight of Trichoderma in liquid culture mycelium under salt stress
Fig.5 Analysis of the high temperature tolerance of T. vir-ide Tv-1511 original strain and its engineered strains A,B:Petri dish test of the heat tolerance of Trichoderma and quantitative statistics of colony diameter. C:Dry weight of Trichoderma liquid culture mycelium under high temperature stress
Fig.6 Analysis of the growth-promoting effects of T. viride Tv-1511 original strain and its engineering strains on Arabidopsis thaliana A:Promoting effects of T. viride and its engineering strains on the growth of A. thaliana. B:Quantitative statistics of fresh weight of Arabidopsis treated with T. viride and its engineered strains. C:Quantitative statistics of leaf areas of Arabidopsis treated with T. viride and its engineering strains
Fig.7 Analysis of the growth-promoting effects of T. viride Tv-1511 original strain and its engineering strains on peppermint(Mentha piperita) A:Promoting effects of Trichoderma viride Tv-1511 and its engineering strains on the growth of peppermint. B:Quantitative statistics of fresh weight of peppermint treated with Trichoderma strains. C:Quantitative statistics of leaf area of peppermint treated with Trichoderma strains
Fig.8 Analysis of the promoting effects of T. viride Tv-1511 original strain and its engineering strains on cucum-ber(Cucumis sativus L.) A:Promoting effects of Trichoderma viride Tv-1511 and its engineering strains on the growth of cucumber. B:Quantitative statistics of fresh weight of cucumber treated with Trichoderma strains. C:Quantitative statistics of leaf areas of cucumber treated with Trichoderma strains
Fig.9 Detection of IAA contents in the fermentation broths of T. viride Tv-1511 original strain and its engineer-ing strains A:Chromatogram of IAA standard. B:Quantitative statistics of IAA content in the fermentation broth of T. viride Tv-1511 and its engineering strains
Fig.10 Analysis of cellulase activities of T. viride Tv-1511 original strain and its engineering strains A:Detection of filter paper enzyme activity in the fermentation broth of T. viride Tv-1511 and its engineering strains. B:Detection of hydroxymethyl cellulase activity in the fermentation broth of T. viride Tv-1511 and its engineering strains. C:Detection of exoglucanase activity in the fermentation broth of T. viride Tv-1511 and its engineering strains. D:Detection of β-glucosidase activity in the fermentation broth of T. viride Tv-1511 and its engineering strains
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