Influence on Expression of Jasmonic Acid Signaling Pathway Gene in Tetranychus urticae Fed on Mite-resistant and Mite-susceptible Cassava Cultivars

doi:10.13560/j.cnki.biotech.bull.1985.2021-0993

Abstract

Abstract:

This study aims to clarify how the mite density and feeding time affect gene expressions of jasmonic acid signaling pathway when Tetranychus urticae fed on mite-resistant and mite-susceptible cassava cultivars,which can not only provide a theoretical basis for elucidating the important role of jasmonic acid signal pathway in cassava resistance to insect,but also can offer references for identifying and evaluating mite resistant germplasm resources. Here,mites with different densities（15,25,35,45,50 and 55 mites/leaf）were inoculated on the leaves of mite-resistant cassava cultivar C1115 and mite-susceptible cassava cultivar Mianbao,respectively. And then the expressions of jasmonic acid signaling pathway genes（DAD1,LOX2,OPR3 and JAR1）were analyzed at 1,2,4 and 8 d post-infestation,respectively. The results showed that the expressions of LOX2,OPR3 and JAR1 in 2 d post-infestation either mite-resistant cassava C1115 and mite-susceptible cassava Mianbao significantly increased compared with those before infested,in addition,the expressions of those three genes were significantly higher in C1115 than Mianbao（P<0.05）,while the expressions of DAD1 showed great fluctuation as the mite density and infestation time increased. This study revealed that under the conditions of relative high mite density and short mite infestation period,the expressions of jasmonic acid signaling pathway genes like LOX2,OPR3 and JAR1 were significantly higher in mite-resistant cassava cultivars compared with the susceptible ones. Those genes might probably correlate with cassava resistance to mite,which needs further investigation.

Key words: Tetranychus urticae, mite-resistant and mite-susceptible cassava cultivars, mite density, mite infestation time, jasmonic acid signaling pathway, gene expression

HAN Zhi-ling, CHEN Qing, LIANG Xiao, WU Chun-ling, LIU Ying, WU Mu-feng, XU Xue-lian. Influence on Expression of Jasmonic Acid Signaling Pathway Gene in Tetranychus urticae Fed on Mite-resistant and Mite-susceptible Cassava Cultivars[J]. Biotechnology Bulletin, 2022, 38(6): 211-220.

Figures/Tables 5

Table 1 qPCR primers for the the expression analysis of jasmonic acid（JA）pathway genes in cassava

引物名称 Primer name	基因登录号Gene ID	序列Sequence（5'-3'）	退火温度Annealing temperature Tm/℃	产物大小Product size/bp
OPR3-F	MANES_03G039700	GTTCACACCTCCAAGGCGA	57.2	103
OPR3-R	MANES_03G039700	AACTCCATCAAACCCAGCTT	58.2	103
DAD1-F	MANES_01G274500	AATGGGCCAATGGTGGAGAG	57.5	118
DAD1-R	MANES_01G274500	CACCGTACCTTTCCAGGACC	59.5	118
JAR1-F	MANES_05G025500	TCATCTACTACGCCCAAGTTTAAGA	58.4	118
JAR1-R	MANES_05G025500	CCATCTCAGCGCCCTTTGTA	58.5	118
LOX2-F	MANES_10G148800	GCCATGGAAACACTTCTTGCT	55.9	297
LOX2-R	MANES_10G148800	CTAGCCCCGTTTTGGGATCA	57.6	297
MeTub-F	MANES_18G123607	ATGTTGAGTTCAATATGCAGATCC	58.1	154
MeTub-R	MANES_18G123607	CCCAGAACTTGGCTCCGATT	61.7	154

Fig. 1 Relative transcriptions of OPR3 gene in mite-resis-tant and mite-susceptible cassava cultivars after infested by mites of different densities at different time A：C1115. B：Mianbao. C：C1115 vs Mianbao. Different lowercase letters indicate that there are significant differences in transcription of OPR3 when cassava was infested by mites of different densities at the same time（P<0.05）,and different capital letters indicate there are significant differences in transcription of OPR3 when cassava were infested by the mites of the same densities at different time（P < 0.05）. The same below

Fig. 2 Relative transcriptions of LOX2 gene in mite-resistant and mite-susceptible cassava cultivars after infested by the mites of different densities at different time

Fig. 3 Relative transcriptions of DAD1 gene in mite-resistant and mite-susceptible cassava cultivars after infested by the mites of different densities at different time

Fig. 4 Relative transcriptions of JAR1 gene in mite-resistant and mite-susceptible cassava cultivars after infested by the mites of different densities at different time

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