Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (8): 127-134.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1519
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LI Xiu-qing1(), HU Zi-yao2, LEI Jian-feng1, DAI Pei-hong2, LIU Chao2, DENG Jia-hui2, LIU Min2, SUN Ling1, LIU Xiao-dong2, LI Yue2()
Received:
2021-12-07
Online:
2022-08-26
Published:
2022-09-14
Contact:
LI Yue
E-mail:549222451@qq.com;liyue6905@126.com
LI Xiu-qing, HU Zi-yao, LEI Jian-feng, DAI Pei-hong, LIU Chao, DENG Jia-hui, LIU Min, SUN Ling, LIU Xiao-dong, LI Yue. Cloning and Functional Analysis of Gene GhTIFY9 Related to Cotton Verticillium Wilt Resistance[J]. Biotechnology Bulletin, 2022, 38(8): 127-134.
基因 Gene | 开放阅读框ORF/bp | 氨基酸 Amino acid | 相对分子质量Relative molecular weight/kD | 理论等电点Theoretical isoelectric point | 平均疏水性Average hydrophobicity | 信号肽 Signal peptide | 亚细胞定位 Subcellular localization | 脂肪系数Fat coefficient | 跨膜结构域 Transmembrane domain |
---|---|---|---|---|---|---|---|---|---|
GhTIFY9 | 594 | 197 | 21.65 | 9.08 | -0.477 | 无 None | 细胞核 Nucleus | 76.63 | 无 None |
Table 1 Bioinformatics analysis of GhTIFY9
基因 Gene | 开放阅读框ORF/bp | 氨基酸 Amino acid | 相对分子质量Relative molecular weight/kD | 理论等电点Theoretical isoelectric point | 平均疏水性Average hydrophobicity | 信号肽 Signal peptide | 亚细胞定位 Subcellular localization | 脂肪系数Fat coefficient | 跨膜结构域 Transmembrane domain |
---|---|---|---|---|---|---|---|---|---|
GhTIFY9 | 594 | 197 | 21.65 | 9.08 | -0.477 | 无 None | 细胞核 Nucleus | 76.63 | 无 None |
Fig.3 Analysis of the expression patterns of GhTIFY9 under the treatment of Verticillium dahliae *:Significant difference at the 0.05 level. **:Significant difference at the 0.01 level. The same as below
Fig. 4 VIGS vector construction of GhTIFY9 A:PCR amplification of target fragment of GhTIFY9. B:Identification of TRV﹕GhTIFY9 vectors by restriction enzyme digestion. M:Trans 2K Plus II DNA marker
Fig. 5 Silencing efficiency detection of GhTIFY9 gene A:The phenotype of silenced GhCLA1 in cotton. B:Silencing efficiency detection of GhCLA1. C:Silencing efficiency detection of GhTIFY9
Fig. 6 Disease resistance identification of GhTIFY9 gene-silenced plants A:Phenotype analysis of plants after GhTIFY9 gene silencing. B:Disease index statistics. C:Identification by stem anatomy. D:Restoration culture of pathogenic bacteria
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