Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (8): 127-134.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1519

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Cloning and Functional Analysis of Gene GhTIFY9 Related to Cotton Verticillium Wilt Resistance

LI Xiu-qing1(), HU Zi-yao2, LEI Jian-feng1, DAI Pei-hong2, LIU Chao2, DENG Jia-hui2, LIU Min2, SUN Ling1, LIU Xiao-dong2, LI Yue2()   

  1. 1. College of Agronomy,Xinjiang Agricultural University,Urumqi 830052
    2. College of Life Sciences,Xinjiang Agricultural University,Urumqi 830052
  • Received:2021-12-07 Online:2022-08-26 Published:2022-09-14
  • Contact: LI Yue E-mail:549222451@qq.com;liyue6905@126.com

Abstract:

The function of TIFY family gene GhTIFY9 in cotton verticillium wilt response was preliminarily explored,which laid a foundation for mining cotton disease resistance related genes and cotton disease resistance breeding. A TIFY family gene GhTIFY9 was obtained by transcriptome screening and cloning. The physicochemical properties of the gene were analyzed by bioinformatics methods. The expression pattern of GhTIFY9 under verticillium wilt induction was analyzed by real-time quantitative polymerase chain reaction(RT-qPCR). Meanwhile,the VIGS vector of the gene was constructed,and its function in the cotton resistance to verticillium wilt was preliminarily explored by virus-induced gene silencing(VIGS)technology. GhTIFY9 was cloned using the cDNA of upland cotton TM-1 as the template. The open reading frame(ORF)of GhTIFY9 was 594 bp,encoding an alkaline hydrophilic protein containing 197 amino acids. The relative molecular weight was 21.65 kD,it was located in the nucleus and belonged to the TIFY subfamily. RT-qPCR analysis showed that GhTIFY9 responded to verticillium wilt induction,and the sensitivity of silent plants to verticillium wilt was significantly enhanced after inhibiting the expression of this gene. GhTIFY9 is a positive regulator of cotton resistance to verticillium wilt.

Key words: cotton verticillium wilt, GhTIFY9, gene cloning, virus-induced gene silencing(VIGS)