Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (9): 127-135.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1549
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WEN Chang(
), LIU Chen, LU Shi-yun, XU Zhong-bing, AI Chao-fan, LIAO Han-peng(), ZHOU Shun-gui
Received:2021-12-14
Online:2022-09-26
Published:2022-10-11
Contact:
LIAO Han-peng
E-mail:2742707020@qq.com;lhp91@126.com
WEN Chang, LIU Chen, LU Shi-yun, XU Zhong-bing, AI Chao-fan, LIAO Han-peng, ZHOU Shun-gui. Biological Characteristics and Genome Analysis of a Novel Multidrug-resistant Shigella flexneri Phage[J]. Biotechnology Bulletin, 2022, 38(9): 127-135.
| 抗生素名称 Antibiotic name | 抗生素浓度Antibiotic concentration /（μg·mL-1） | 药敏性Sensitivity |
|---|---|---|
| 四环素 Tetracycline | 2 | R |
| 氯霉素 Chloramphenicol | 16 | R |
| 氨苄青霉素 Ampicillin | 100 | R |
| 卡那霉素 Kanamycin | 100 | S |
| 阿莫西林 Amoxicillin | 64 | R |
| 硫酸链霉素 Streptomycin | 30 | R |
| 庆大霉素 Gentamicin | 10 | R |
| 红霉素 Erythromycin | 15 | R |
| 利福平 Rifampin | 100 | R |
Table 1 Antibiotic sensitivity of host bacteria
| 抗生素名称 Antibiotic name | 抗生素浓度Antibiotic concentration /（μg·mL-1） | 药敏性Sensitivity |
|---|---|---|
| 四环素 Tetracycline | 2 | R |
| 氯霉素 Chloramphenicol | 16 | R |
| 氨苄青霉素 Ampicillin | 100 | R |
| 卡那霉素 Kanamycin | 100 | S |
| 阿莫西林 Amoxicillin | 64 | R |
| 硫酸链霉素 Streptomycin | 30 | R |
| 庆大霉素 Gentamicin | 10 | R |
| 红霉素 Erythromycin | 15 | R |
| 利福平 Rifampin | 100 | R |
Fig.1 Plaque（A）and morphology by TEM（B）of phage P003
Fig.2 Optimal multiplicity of infection（A）and one-step growth curve of phage P003（B）
Fig. 3 Temperature (A), pH (B) and UV (C) stability of phage P003
| 菌株 Bacterial strain | 敏感性 Sensitivity |
|---|---|
| Shigella flexneri B003 | + |
| Shigella flexneri M001 | — |
| Shigella flexneri M002 | — |
| Shigella flexneri M003 | — |
| Escherichia coli HB101 | — |
| Escherichia coli DH5α | — |
| Escherichia coli K12 | — |
| Escherichia coli KN | — |
| Escherichia coli OP50 | — |
| Escherichia LFHY_s J001 | — |
| Escherichia hermannii | — |
| Enterobacter cloacae subsp | — |
| Serratia nematodiphila | — |
| Serratia marcescens | — |
| Klebsiella africana | — |
| Klebsiella pneumoniae subsp | — |
Table 2 Lytic range of phage P003
| 菌株 Bacterial strain | 敏感性 Sensitivity |
|---|---|
| Shigella flexneri B003 | + |
| Shigella flexneri M001 | — |
| Shigella flexneri M002 | — |
| Shigella flexneri M003 | — |
| Escherichia coli HB101 | — |
| Escherichia coli DH5α | — |
| Escherichia coli K12 | — |
| Escherichia coli KN | — |
| Escherichia coli OP50 | — |
| Escherichia LFHY_s J001 | — |
| Escherichia hermannii | — |
| Enterobacter cloacae subsp | — |
| Serratia nematodiphila | — |
| Serratia marcescens | — |
| Klebsiella africana | — |
| Klebsiella pneumoniae subsp | — |
Fig.4 Genome profile of phage P003
Fig. 5 Gene function annotation of phage P003
| Accession No. | Escherichia phage | Coverage/% | Identity/% |
|---|---|---|---|
| NC_048194.1 | vB_EcoM_WFH | 96 | 99.18 |
| MH051335.1 | vB_EcoM-Ro157lw | 94 | 97.22 |
| JX128258.1 | ECML-117 | 92 | 97.04 |
| MK903282.1 | Mansfield | 92 | 96.36 |
| NC_048073.1 | FEC19 | 94 | 95.32 |
Table 3 Comparative analysis of phage P003 with virus database based on genome
| Accession No. | Escherichia phage | Coverage/% | Identity/% |
|---|---|---|---|
| NC_048194.1 | vB_EcoM_WFH | 96 | 99.18 |
| MH051335.1 | vB_EcoM-Ro157lw | 94 | 97.22 |
| JX128258.1 | ECML-117 | 92 | 97.04 |
| MK903282.1 | Mansfield | 92 | 96.36 |
| NC_048073.1 | FEC19 | 94 | 95.32 |
Fig. 6 Whole-gene sequence alignment of phage P003 with 3 Escherichia phages
Fig.7 Phylogenetic tree based on the major capsid protein of phage P003
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