Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (1): 150-156.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0479
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RUAN Hang1,2(), DUO Hao-yuan1, FAN Wen-yan1, LV Qing-han1, JIANG Shu-jun1(), ZHU Sheng-wei2()
Received:
2022-04-17
Online:
2023-01-26
Published:
2023-02-02
Contact:
JIANG Shu-jun,ZHU Sheng-wei
E-mail:3309952272@qq.com;jsjfwy@sohu.com;zhusw@ibcas.ac.cn
RUAN Hang, DUO Hao-yuan, FAN Wen-yan, LV Qing-han, JIANG Shu-jun, ZHU Sheng-wei. Role of the AtERF49 in the Responses to Salt-alkali Stress in Arabidopsis[J]. Biotechnology Bulletin, 2023, 39(1): 150-156.
引物名称Primer name | 引物序列Primer sequence(5'-3') |
---|---|
RAB18-F | TCCAGCTCTAGCTCGGAGGATGA |
RAB18-R | GGATCCCATGCCGCCCATCG |
RD29A-F | GCCGGAATCTGACGGCGGTT |
RD29A-R | CCCGTCGGCACATCCTTGTCG |
AtUBC30-F | TCACTTCCCACCAGATTACCC |
AtUBC30-R | TCGACAGAAGAACCTTGGATACG |
AtERF49-F | AGACTACACCAAGCAGCAACACC |
AtERF49-R | TTGGATGAACACGGCGACTCAG |
rbcl-F | TTGGCAGCATTCCGAGTAACTCCT |
rbcl-R | CTGGTAAGTCCATCGGTCC |
psaA-F | CTACTTTGCCACCCACTGC |
psaA-R | TGAGTGCTTTAGGGCGTCC |
Table 1 Primers used for fluorescence quantitative PCR
引物名称Primer name | 引物序列Primer sequence(5'-3') |
---|---|
RAB18-F | TCCAGCTCTAGCTCGGAGGATGA |
RAB18-R | GGATCCCATGCCGCCCATCG |
RD29A-F | GCCGGAATCTGACGGCGGTT |
RD29A-R | CCCGTCGGCACATCCTTGTCG |
AtUBC30-F | TCACTTCCCACCAGATTACCC |
AtUBC30-R | TCGACAGAAGAACCTTGGATACG |
AtERF49-F | AGACTACACCAAGCAGCAACACC |
AtERF49-R | TTGGATGAACACGGCGACTCAG |
rbcl-F | TTGGCAGCATTCCGAGTAACTCCT |
rbcl-R | CTGGTAAGTCCATCGGTCC |
psaA-F | CTACTTTGCCACCCACTGC |
psaA-R | TGAGTGCTTTAGGGCGTCC |
Fig. 2 Overexpression of AtERF49 gene increasing Arabidopsis resistance to Saline-alkali stress A: Phenotypic analysis of AtERF49 transgenic Arabidopsis phenotype with Saline-alkali stress, the scale is 2 cm. B: Relative expression of AtERF49 in wild type and transgenic lines
Fig. 4 Effects of Saline-alkali stress on the chlorophyll fluorescence parameters of leaves of AtERF49 transgenic Arabidopsis A: Energy dissipation quantum yield. B: Actual quantum yield of photosystem II. C: Coefficient of non-photochemical quenching. D: Coefficient of photochemical quenching
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