Screening, Identification and Growth-promoting Effect of Antagonistic Bacillus spp. Against Cucumber Fusarium solani

doi:10.13560/j.cnki.biotech.bull.1985.2022-0522

Abstract

Abstract:

Cucumber root rot caused by Fusarium solani seriously affects the sustainable development of cucumber industry. In order to obtain high-efficiency antagonistic Bacillus spp. against cucumber root rot and to clarify its growth-promoting effect, Bacillus strains were isolated from the rhizosphere soil of cucumber plants, and the strains with high antagonistics to F. solani were screened, and the morphological, physiological and biochemical, genetic characteristics and plant growth-promoting characteristics were evaluated. The results showed that strain XY-1, XY-13 and XY-53 demonstrated significant inhibitory effects on F. solani, with inhibitory rates of 65.90%, 66.13% and 60.83%, respectively. XY-1 and XY-13 were identified as B. amyloliquefaciens, and XY-53 as B. subtilis. All the three strains showed phosphate-solubilizing ability, nitrogen-fixing ability, and produced protease and ACC deaminase. In the seedlings growth promotion test, inoculation with strain XY-1, XY-13 and XY-53 effectively promoted the growth of cucumber seedlings, and the seedlings’ stem lengths increased by 37.17%, 27.14% and 34.94%, respectively compared with control, and the chlorophyll content increased by 31.37%, 40.48% and 43.43%, the fresh weight of above ground increased by 33.03%, 38.81% and 51.52%, and the dry weight of above ground increased by 28.66%, 29.03% and 46.27%, respectively. In pot experiment for control efficiency, the concentrations of 10⁸ CFU/mL showed the best control efficiency on the cucumber root rot after 10 d of inoculation with XY-1, XY-13 and XY-53 different concentrations bacteria suspensions, and it was 65.12%, 72.09% and 82.86%, respectively. Moreover, the strain XY-1, XY-13 and XY-53 promoted the increase of seedlings stem length and chlorophyll content under the infection of pathogenic fungi. In conclusion, XY-1, XY-13 and XY-53 can be used as potential biocontrol resources to control cucumber seedlings root rot caused by F. solani and to simultaneously promote the growth of seedlings for sustainable and efficient cucumber production.

Key words: biological control, Bacillus spp., Fusarium solani, cucumber root rot, growth promotion

YANG Dong-ya, QI Rui-xue LI, Zhao-xuan , LIN Wei, MA Hui, ZHANG Xue-yan. Screening, Identification and Growth-promoting Effect of Antagonistic Bacillus spp. Against Cucumber Fusarium solani[J]. Biotechnology Bulletin, 2023, 39(2): 211-220.

Figures/Tables 10

References 44

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抑菌率Inhibition rate/%	菌株数目Number of strains
60-70	5
50-60	59
40-50	36
30-40	51
30以下	192

抑菌率Inhibition rate/%	菌株数目Number of strains
60-70	5
50-60	59
40-50	36
30-40	51
30以下	192

菌株Strain	菌落直径Colony diameter/mm	抑菌率Inhibition rate/%
XY-1	24.67±0.17b	65.90±0.23a
XY-13	24.50±0.29b	66.13±0.40a
XY-53	28.33±0.17a	60.83±0.23b

菌株Strain	菌落直径Colony diameter/mm	抑菌率Inhibition rate/%
XY-1	24.67±0.17b	65.90±0.23a
XY-13	24.50±0.29b	66.13±0.40a
XY-53	28.33±0.17a	60.83±0.23b

菌株 Strain	颜色 Color	菌落形状 Colony shape	边缘 Edge	表面光滑凸起与否 Surface is smooth and bump or not	表面干湿 Surface is dry or wet	色素产生 Pigment
XY-1	白色	圆形	光滑	表面粗糙不规则，隆起有褶皱	干燥	无色素产生
XY-13	白色	圆形	光滑	表面隆起有褶皱	湿润	无色素产生
XY-53	乳白色	圆形	光滑	表面凸起	湿润	无色素产生