Genome-wide Identification and Stress Response Analysis of Soybean GS Gene Family

doi:10.13560/j.cnki.biotech.bull.1985.2024-0205

Abstract

Abstract:

【Objective】Glutamine synthetase（GS）is a key enzyme in the GS-GOGAT cycle of nitrogen metabolism. This work is aimed to investigate the family members of GS in soybean and the response to external stress.【Method】Based on bioinformatics methods, we comprehensively identified GS genes in soybean（Glycine max）, clarified the location and structure of soybean GmGSs genes, the physicochemical properties of proteins, and the tissue expression patterns, and also studied the responses to abiotic stresses.【Result】The total of eight GS genes were identified from the soybean, located on eight chromosomes, corresponding to coding amino acid sequences with lengths ranging from 356 to 432 aa. Among the ten conserved motifs, all eight GmGS contained nine conserved motifs, and GmGS7 and GmGS8 had one more motif 10 conserved motif than other members. Analysis of promoter's cis-acting elements showed that the promoters of GmGSs contained abundant light-responsive elements, hormone-responsive elements, and adversity stress-responsive elements. Transcriptome data analysis showed that GmGSs genes were expressed in all tissues, with GmGS3 and GmGS4 having higher expression in all tissues. Fluorescence quantitative qPCR results revealed that GmGS4, GmGS5, and GmGS7 of soybean GS family responded most significantly to the late stage of low concentration ammonium treatment after different concentrations of ammonium chloride. And after high salt stress treatment, the expression of GmGS5 decreased in the root, stem and leaf tissues; while the expression of GmGS7 increased in root and stem tissues.【Conclusion】There are eight GS members in soybean, of which GmGS7 is involved in response to both ammonium chloride treatment and salt stress.

Key words: soybean, glutamine synthetase, bioinformatics analysis, ammonium chloride, salt stress

WU Shuai, XIN Yan-ni, MAI Chun-hai, MU Xiao-ya, WANG Min, YUE Ai-qin, ZHAO Jin-zhong, WU Shen-jie, DU Wei-jun, WANG Li-xiang. Genome-wide Identification and Stress Response Analysis of Soybean GS Gene Family[J]. Biotechnology Bulletin, 2024, 40(8): 63-73.

Figures/Tables 10

References 33

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基因ID Gene ID	正向引物 Forward primer（5'-3'）	反向引物Reverse primer（5'-3'）
GmCYP2	CGGGACCAGTGTGCTTCTTCA	CCCCTCCACTACAAAGGCTCG
GmGS1	TAACACCAAACAGAGTCATTCACC	TGAGGTTGATGAGATCCGAAA
GmGS2	GACTAATTTCCGGGGTTTCG	GGAGACCTTTTTCTTCCTCACAG
GmGS3	GCTTTTCTTAGTAGGATTTGGTCTC	TAACAATCGGAAAACGAGGGA
GmGS4	GTGGAAGCCATGAGCAAAACT	CGAGGGAAAGGAATAGAAAACA
GmGS5	TTGGAAACCATAAGCAGCCTC	GCCAAGCATTGAAGTGTGAGA
GmGS6	GCAACGTCAAAACAATCACATG	AACAACAGGCGAGGTAGTCACA
GmGS7	GCTGGTGTTGTGCTCTCTCT	TCTTCCCACACGGATTGAGC
GmGS8	ACCCAAAGGTCCAAGCAG	CCAGGATAGCCACCAACG

基因ID Gene ID	正向引物 Forward primer（5'-3'）	反向引物Reverse primer（5'-3'）
GmCYP2	CGGGACCAGTGTGCTTCTTCA	CCCCTCCACTACAAAGGCTCG
GmGS1	TAACACCAAACAGAGTCATTCACC	TGAGGTTGATGAGATCCGAAA
GmGS2	GACTAATTTCCGGGGTTTCG	GGAGACCTTTTTCTTCCTCACAG
GmGS3	GCTTTTCTTAGTAGGATTTGGTCTC	TAACAATCGGAAAACGAGGGA
GmGS4	GTGGAAGCCATGAGCAAAACT	CGAGGGAAAGGAATAGAAAACA
GmGS5	TTGGAAACCATAAGCAGCCTC	GCCAAGCATTGAAGTGTGAGA
GmGS6	GCAACGTCAAAACAATCACATG	AACAACAGGCGAGGTAGTCACA
GmGS7	GCTGGTGTTGTGCTCTCTCT	TCTTCCCACACGGATTGAGC
GmGS8	ACCCAAAGGTCCAAGCAG	CCAGGATAGCCACCAACG

基因名称 Gene name	基因座位ID Gene locus ID	转录本ID Transcript ID	氨基酸数量Number of amino acids/aa	理论等电点Theoretical pI	分子量Molecular weight/kD	不稳定系Instability index	亲水平均数 Grand average of hydropathicity	亚细胞定位 Subcellular localization
GmGS1	Glyma.09g173200	KRH39032	356	5.22	39212.02	40.29	-0.467	Cytoplasmic
GmGS2	Glyma.07g104500	KRH48675	356	5.32	39148	40.49	-0.442	Cytoplasmic
GmGS3	Glyma.11g215500	KRH30930	356	5.48	38990.93	37.96	-0.374	Cytoplasmic
GmGS4	Glyma.18g041100	KRG97948	356	5.48	39140.98	39.47	-0.425	Cytoplasmic
GmGS5	Glyma.14g213300	KRH17324	356	6.12	39208.29	38.63	-0.444	Cytoplasmic
GmGS6	Glyma.02g244000	KRH72976	356	6.03	39326.47	39.75	-0.428	Cytoplasmic
GmGS7	Glyma.13g210800	KRH48676	432	6.42	47653.72	42.55	-0.466	Chloroplast
GmGS8	Glyma.15g102000	KRH30931	432	6.73	47691.83	43.16	-0.458	Chloroplast

基因名称 Gene name	基因座位ID Gene locus ID	转录本ID Transcript ID	氨基酸数量Number of amino acids/aa	理论等电点Theoretical pI	分子量Molecular weight/kD	不稳定系Instability index	亲水平均数 Grand average of hydropathicity	亚细胞定位 Subcellular localization
GmGS1	Glyma.09g173200	KRH39032	356	5.22	39212.02	40.29	-0.467	Cytoplasmic
GmGS2	Glyma.07g104500	KRH48675	356	5.32	39148	40.49	-0.442	Cytoplasmic
GmGS3	Glyma.11g215500	KRH30930	356	5.48	38990.93	37.96	-0.374	Cytoplasmic
GmGS4	Glyma.18g041100	KRG97948	356	5.48	39140.98	39.47	-0.425	Cytoplasmic
GmGS5	Glyma.14g213300	KRH17324	356	6.12	39208.29	38.63	-0.444	Cytoplasmic
GmGS6	Glyma.02g244000	KRH72976	356	6.03	39326.47	39.75	-0.428	Cytoplasmic
GmGS7	Glyma.13g210800	KRH48676	432	6.42	47653.72	42.55	-0.466	Chloroplast
GmGS8	Glyma.15g102000	KRH30931	432	6.73	47691.83	43.16	-0.458	Chloroplast