Bioinformatics and Expression Pattern Analysis of Glutathione S-transferase in Populus davidiana × P. bolleana

doi:10.13560/j.cnki.biotech.bull.1985.2024-0430

Abstract

Abstract:

Objective To provide a basis for further study of the function of glutathione transferase (GST) gene in woody plants, the expression pattern of GST genes from Populus davidiana × P. bolleana were analyzed. Method The GST genes were cloned and analyzed by bioinformatics of Populus davidiana × P. bolleana. The expression pattern of GST genes under phytohormone and abiotic stresses were analyzed by real-time PCR. Result Three GST gene members from Populus davidiana × P. bolleana were cloned. The lengths of these genes were 678, 660 and 690 bp, encoding amino acids of 225, 219 and 229 aa, respectively. Meanwhile, all three proteins have the typical structure of glutathione transferase, and their promoter regions have elements that can respond to biotic or abiotic stresses as well as plant hormones. In addition, the expressions of these three genes were differently induced by exogenous phytohormones or abiotic stresses. Conclusion All the three PdbGSTs may respond to abiotic stresses or the induction of exogenous phytohormone, indicating that they may play roles in response to the stresses in poplar.

Key words: glutathione transferase, bioinformatics analysis, gene expression pattern, Populus davidiana × P. bolleana

HUANG Ying, YU Wen-jing, LIU Xue-feng, DIAO Gui-ping. Bioinformatics and Expression Pattern Analysis of Glutathione S-transferase in Populus davidiana × P. bolleana[J]. Biotechnology Bulletin, 2025, 41(2): 248-256.

Figures/Tables 8

Table 1 Primers used in this study

基因名称 Gene name	正向引物序列 Forward primer （5′-3′）	反向引物序列 Reverse primer （5′-3′）	用途 Purpose
PdbGST1	ATGGCAGAGGAAGTGAAGGTCTTTAG	TCATTTATGTGTTTCTTTTCTAAGAC	基因克隆
PdbGST2	ATGGCAGGAATAAAACTACTAGATTC	TCATTCAATCCCAAGCTTCTTCCTG	基因克隆
PdbGST3	ATGGCAAACACAGAGCTGGTGAAGC	TCAAGTCCGAAACCAGTTATGAGG	基因克隆
PdbGST1-RT	ACCAGAAAGCCAATGCTCGT	GCCTCCCCACCGAAGAATTT	荧光定量PCR
PdbGST2-RT	GGTCTGGAATGACAGGTCTCCTTTGC	GGGCTTGTCTCCAAGCTCTCCTTC	荧光定量PCR
PdbGST3-RT	CCACTGATGCCTCAAGATCCTTACG	CCAGGTAGCCAATGTTCTCTCCTCC	荧光定量PCR
PdbEF1-α	TGGGTCGTGTTGAAACTGGTGT	GGCAGGATCGTCCTTGGAGTTC	荧光定量PCR
Pdbactin	GCTGAGAGATTCCGTTGCCCTG	GGCGGTGATCTCCTTGCTCATT	荧光定量PCR

Table 2 Characteristics of PdbGSTs protein

基因名称

Gene name

基因长度

Length/bp

氨基酸长度

Protein/aa

蛋白质相对分子质量

Mw/kD

等电点

Isoelectric point

不稳定系数

Instability index （II）

亲疏水性

GRAVY

亚细胞定位

Localization

PdbGST1

PdbGST2

PdbGST3

Fig. 1 Multiple alignment of PdbGST proteinKAG6769001, KAG6793805, KAG6757347: Populus tomentosa; XP_034919935, XP_034900369, XP_034914570: P. alba; XP_011004483, XP_011030495: P. euphratica; ANO39980, ANO40011, ANO40009: P. yatungensis; KAH8506839, KAH8495610: P. deltoides; KAJ6992085, KAJ7014641, KAJ6981522: P. alba×P. berolinensis; XP_002307924, XP_006370194, XP_006378334: P. trichocarpa

Fig. 2 Phylogenetic tree analysis of PdbGST in poplarRed stars indicate PdbGST protein

Fig. 3 Analysis of promoter sequences of PdbGST

Fig. 4 Tissues expression analysis of PdbGSTs geneDifferent lowercase letters indicate significant differences between a gene in different tissues （P<0.05）

Fig. 5 Expression profiles of PdbGSTs under different phytohormones inductionA: SA treatment; B: ABA treatment; C: ACC treatment. Different lowercase letters indicate significant differences between a gene during the treatment （P<0.05）. The same below

Fig. 6 Expression profiles of PdbGSTs under abiotic stressesA: PEG6000; B: mannitol; C: NaCl

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