Cloning and Function Analysis of Gene GhPYR1 in Gossypium hirsutum L.

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.012

Abstract

Abstract: The phytohormone abscisic acid(ABA)plays an important role in response to drought, salinity and other abiotic stress.It is also a key regulator involved in different plant developmental stages such as seed germination, root elongation, and bud dormancy.The PYR/PYL/RCAR protein family is the ABA receptor, which can initiate the ABA signaling pathway and induce the expression of ABA response genes.In this study, gene GhPYR1 was cloned from Gossypium hirsutum L.with electronic cloning and RT-PCR technology.GhPYR1 protein exhibited 73% sequence identity with Arabidopsis AtPYR1 protein.Protein sequence alignment among GhPYR1 and 14 family members of PYR/PYL/RCAR in Arabidopsis as well as the construction of phylogenetic tree revealed that gene GhPYR1 was most closely related to subfamily III of PYR/PYL/RCAR protein in Arabidopsis.T₃ generation Arabidopsis over-expressing gene GhPYR1were more sensitive to exogenous ABA, as its seed germination and early root growth lagged behind than wild type.The germination of transgenic seeds was more strongly inhibited by high salt and drought stress, however, the transgenic lines were significantly better than the wild ones in the seeding stage.Moreover, the ABA-responsive gene RD29A and RAB18 in transgenic Arabidopsis were significantly induced by ABA compared to the wild type.The above results show that the protein encoded by gene GhPYR1 is the receptor of ABA and over-expression of GhPYR1 can improve the sensitivity of plant to ABA and enhance the ability to respond to stress in plants.

Key words: Gossypium hirsutum L. , GhPYR1 gene, bioinformatics analysis, physiological function analysis

LIU Yan, MENG Zhi-gang, SUN Guo-qing, WANG Yuan, ZHOU Tao, GUO San-dui, ZHANG Rui. Cloning and Function Analysis of Gene GhPYR1 in Gossypium hirsutum L.[J]. Biotechnology Bulletin, 2016, 32(2): 90-99.

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