PIF4-Regulated Thermo-responsive Genes in Arabidopsis

doi:10.13560/j.cnki.biotech.bull.1985.2018-0211

Abstract

Abstract: Plant growth and development programs are coordinated with various internal factors such as phytohormones and environmental signals such as fluctuated temperatures. The Phytochrome B（PHYB）interacting protein PIF4 promotes plant morphogenesis at elevated ambient temperature,however,genome-wide downstream genes of PIF4 during this response have not been reported. In this study,we confirmed the important role of PIF4 in thermoresponsive morphogenesis in Arabidopsis,and 248 PIF4-dependent and 21 PIF4 partially dependent downstream genes at elevated ambient temperature were further revealed by RNA-Seq,including those genes in auxin responsive pathway. 74 possible direct targets of PIF4 in thermomorphogenesis were identified by combining the previous published CHIP-Seq data and our RNA-Seq data,and 6 out of 7 were confirmed by ChIP-qPCR. Thus,our study has revealed PIF4 downstream genes during thermomorphogenesis,which are important for a better understanding of plant responses to elevated ambient temperature.

Key words: ambient high temperature, hypocotyl elongation, PIF4, downstream gene, RNA-Seq, ChIP-qPCR

WANG Shuo, DING Lan, LIU Jian-xiang, HAN Jia-jia. PIF4-Regulated Thermo-responsive Genes in Arabidopsis[J]. Biotechnology Bulletin, 2018, 34(7): 57-65.

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