Screening of Strains for Enhancing Oil Recovery in Northern Shaanxi and Evaluation of Their Degradabilities

doi:10.13560/j.cnki.biotech.bull.1985.2018-0654

Abstract

Abstract: In order to screen the strains that can enhance oil recovery by using metabolites,using enrichment and dilution plate method,strains of degrading crude oil as sole carbon source were screened from nearby long-time petroleum-polluted waters and oily sludge in the northern Shaanxi,and their characteristics,including their growth condition,oil displacement capability,surface active substance of metabolites,surface tension and emulsification,and the degradation of crude oil were analyzed,respectively. Ten strains of bacteria were screened from wastewater containing oil and oily sludge. Further,6 dominant bacteria were selected as test ones for follow-up study,6 strains of bacteria resulted in the physicochemical properties of crude oil changed. Crude oil viscosity reduced by 49.02% after strain B and crude oil reacted,the composite bacteria of strain A-08,strain 5-13,strain 1-2,strain A,and strain A-08 the crude oil emulsified into circular small particles,and the degradation rate reached 43.25%-60.00%.

Key words: enhancing oil recovery, metabolites, viscosity reduction, surface tension, emulsification

DENG Zhen-shan, SHANG Rong-fang, CHEN Kai-kai, WANG Xiao-jiang. Screening of Strains for Enhancing Oil Recovery in Northern Shaanxi and Evaluation of Their Degradabilities[J]. Biotechnology Bulletin, 2019, 35(2): 73-79.

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