Development of SSR Molecular Markers with Sorghum Polymorphism Using Re-sequencing

doi:10.13560/j.cnki.biotech.bull.1985.2019-0692

Abstract

Abstract: SSR marker is widely applied in molecule-assisted breeding due to its low cost and easy operation. Currently,sorghum SSR marker is based on sequencing,and although the genome sequence of American sorghum BTX623 is completed,the efficiency is low in screening polymorphism markers in applications. In this study,26 sorghum materials composed of sterile lines and restorer lines were re-sequenced and analyzed by bioinformatics,and 24 441 SSR markers containing at least 2 polymorphic types in 26 materials were developed. These SSR markers generated 2-7 genotypes in 26 sorghum varieties,including 16 694 markers for 2 genotypes and only 77 markers for 7 genotypes. In addition,6 673 polymorphic SSRs at the single-copy gene were screened. Fifty pairs of SSRs on the 10 evenly-covered sorghum chromosomes were randomly selected as bridge markers,and Liaoning sorghum hybrid Liaonuo 3 was used for test. Among them,49 pairs of primers were amplified,the success rate of which achieved 98%. Subsequent test with 2 sorghum varieties and 74 mini-core germplasm showed that 18 pairs of SSR markers among 50 pairs of SSRs presented polymorphism between the 2 varieties. One selected SSR marker generated 8 genotypes in 74 mini-core germplasm. This study demonstrates that re-sequencing sterile lines and restorer lines may effectively develop SSR markers for Chinese sorghum breeding.

Key words: sorghum, SSR, re-sequencing, polymorphic primer, molecular marker

WANG Ping, WANG Chun-yu, ZHANG Li-xia, CONG Ling, ZHU Zhen-xing, LU Xiao-chun. Development of SSR Molecular Markers with Sorghum Polymorphism Using Re-sequencing[J]. Biotechnology Bulletin, 2019, 35(11): 217-223.

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