Research Progress on Biosynthesis and Metabolic Regulation of Ganoderic Acids

doi:10.13560/j.cnki.biotech.bull.1985.2021-0734

Abstract

Abstract:

Ganoderic acid（GA）is the secondary metabolites from medicinal Ganoderma lucidum and main active component of it,which has been studied deeply in anti-cancer and other medical fields. However,due to the low yield of GAs,long growth cycle of spores and fruiting bodies,and high growth environment requirements,and the submerged liquid fermentation of G. Lucidum was developed. Although some breakthroughs have been made,the problem of low GAs yield is still existed. The analysis and regulation of GAs biosynthesis pathway is of significance for solving low yield in the fermentation of producing GAs. Therefore,this paper reviews the research progress of GAs biosynthesis and metabolic regulation,proposes the direction of future research,and provides a reference for further improving GAs yield through genetic engineering and metabolic pathway regulation.

Key words: ganoderic acids, synthesis metabolism, genetic engineering, metabolic regulation

YUAN Kai, HE Wei, YANG Yun-li, ZHU Wei-yu, PENG Chao, AN Tai, LI Li, ZHOU Wei-qiang. Research Progress on Biosynthesis and Metabolic Regulation of Ganoderic Acids[J]. Biotechnology Bulletin, 2021, 37(8): 46-54.

Figures/Tables 3

References 51

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信号转导调控通路 Signal transduction regulatory pathway	作用方式 Method of operation	代谢水平变化 Changes in metabolic levels	灵芝酸含量变化 Changes in ganoderic acid content	文献 Reference
钙调磷脂酶信号	添加10 mmol/L Ca²⁺	cam、cna、crz1、hmgr、sqs、lss转录水平上调	GAs含量增加3.7倍;灵芝酸MK、灵芝酸T、灵芝酸S和灵芝酸Me分别增加2.6倍、4.5倍、3.2倍和3.8倍	[35]
	添加10 mmol/L Mn²⁺	cam、cna、crz1、pmr1、hmgr、sqs、lss转录水平上调	GAs含量增加2.2倍	[36]
	添加100 mmol/L Na⁺	hmgr转录水平上调20倍,sqs上调109倍,lss上调4倍	GAs含量增加2.8倍	[37]
活性氧信号	通过基因工程获得Glswi6沉默菌株	NAHD氧化酶活性下降48%,hmgr、sqs、lss转录水平均下调	GAs含量降低25%	[38]
	在Glswi6沉默菌株发酵过程中回补1 mmol/L H₂O₂	NAHD氧化酶活性和hmgr、sqs、lss转录水平恢复至野生型菌株水平	GAs含量恢复至野生型菌株水平	[38]
	通过基因工程获得Glskn7沉默菌株Skn7i-5和Skn7i-7	H₂O₂含量上升73.7%和76.4%,ROS水平是原始菌株的1.78和1.82倍,hmgr转录水平上升56%、52%,sqs上调49%、53%,lss上调95%、93%	GAs含量分别提高52.1%和55.9%	[39]
	在Glskn7沉默菌株发酵过程中回补加入5 mmol/L N-乙酰半胱氨酸	--	GAs含量降低至与野生型菌株持平	[39]
	通过基因工程获得PacC沉默菌株	胞内SOD、CAT、Gpx、Apx等抗氧化酶活性下降幅度超过40%,ROS水平上升幅度超过2倍	GAs含量明显提升	[40]
	当转录因子PacC沉默时,回补1 mmol/L NAC 和 2 mmol/L维生素C时	--	GAs含量降低至与出发菌株持平	[41]
细胞壁完整性信号	通过基因工程获得MAPK4沉默菌株	hmgr、sqs、lss转录水平下调	GAs含量最大降低40%	[42]
茉莉酮酸（JA）及其衍生物信号	添加254 μmol/L的MeJA	fps、sqs、hmgr、mvd、se转录水平上调	GAs含量提高45.3%	[32]
NO信号	添加5 mmol/L的NO供体	sqs转录水平上调2.43倍	灵芝三帖含量提高40.94%	[33]
cAMP信号	添加80 mmol/L咖啡因	尽管检测结果显示hmgr、sqs、lss转录水平下调,但是GAs的生物合成代谢表现出正向结果,目前作者仍在探究其中原因	GAs含量增长3.6倍	[34]

信号转导调控通路 Signal transduction regulatory pathway	作用方式 Method of operation	代谢水平变化 Changes in metabolic levels	灵芝酸含量变化 Changes in ganoderic acid content	文献 Reference
钙调磷脂酶信号	添加10 mmol/L Ca²⁺	cam、cna、crz1、hmgr、sqs、lss转录水平上调	GAs含量增加3.7倍;灵芝酸MK、灵芝酸T、灵芝酸S和灵芝酸Me分别增加2.6倍、4.5倍、3.2倍和3.8倍	[35]
	添加10 mmol/L Mn²⁺	cam、cna、crz1、pmr1、hmgr、sqs、lss转录水平上调	GAs含量增加2.2倍	[36]
	添加100 mmol/L Na⁺	hmgr转录水平上调20倍,sqs上调109倍,lss上调4倍	GAs含量增加2.8倍	[37]
活性氧信号	通过基因工程获得Glswi6沉默菌株	NAHD氧化酶活性下降48%,hmgr、sqs、lss转录水平均下调	GAs含量降低25%	[38]
	在Glswi6沉默菌株发酵过程中回补1 mmol/L H₂O₂	NAHD氧化酶活性和hmgr、sqs、lss转录水平恢复至野生型菌株水平	GAs含量恢复至野生型菌株水平	[38]
	通过基因工程获得Glskn7沉默菌株Skn7i-5和Skn7i-7	H₂O₂含量上升73.7%和76.4%,ROS水平是原始菌株的1.78和1.82倍,hmgr转录水平上升56%、52%,sqs上调49%、53%,lss上调95%、93%	GAs含量分别提高52.1%和55.9%	[39]
	在Glskn7沉默菌株发酵过程中回补加入5 mmol/L N-乙酰半胱氨酸	--	GAs含量降低至与野生型菌株持平	[39]
	通过基因工程获得PacC沉默菌株	胞内SOD、CAT、Gpx、Apx等抗氧化酶活性下降幅度超过40%,ROS水平上升幅度超过2倍	GAs含量明显提升	[40]
	当转录因子PacC沉默时,回补1 mmol/L NAC 和 2 mmol/L维生素C时	--	GAs含量降低至与出发菌株持平	[41]
细胞壁完整性信号	通过基因工程获得MAPK4沉默菌株	hmgr、sqs、lss转录水平下调	GAs含量最大降低40%	[42]
茉莉酮酸（JA）及其衍生物信号	添加254 μmol/L的MeJA	fps、sqs、hmgr、mvd、se转录水平上调	GAs含量提高45.3%	[32]
NO信号	添加5 mmol/L的NO供体	sqs转录水平上调2.43倍	灵芝三帖含量提高40.94%	[33]
cAMP信号	添加80 mmol/L咖啡因	尽管检测结果显示hmgr、sqs、lss转录水平下调,但是GAs的生物合成代谢表现出正向结果,目前作者仍在探究其中原因	GAs含量增长3.6倍	[34]