Effect of Premature Bolting on the Rhizosphere Soil Microenvironment of Angelica sinensis

doi:10.13560/j.cnki.biotech.bull.1985.2022-1446

Abstract

Abstract:

The changes of rhizosphere soil microenvironment during Angelica sinensis bolting were explored to provide new ideas for solving the problem of premature bolting. A. sinensis was taken as the research object, the soil properties, bacterial community and metabolite in the rhizosphere soil were determined by 16S rDNA and GC-MC techniques in bolting and unbolting A. sinensis, and the correlation among them was studied. The results show that nitrate nitrogen content in the rhizosphere soil of bolting groups were significantly higher than those of unbolting ones. There was no difference between bolting and unbolting groups in the diversity of rhizosphere soil bacterial community and the structure of dominant bacteria, thus the bacterial community was alike. However, there were significantly differences among 10 non-dominant genera. There were 66 metabolites in the rhizosphere soil of bolting groups, which were different from the unbolting ones, 52 of which were significantly up and 14 significantly down. The differential metabolites were mainly concentrated in 7 related pathways of amino acid metabolism, heterogeneous biodegradation and metabolism, and lipid metabolism. The nitrate nitrogen content, Phaselicystis, Rubellimicrobium were significantly negatively correlated to the differential metabolites of bolting groups（P<0.05）. The nitrate nitrogen was mainly negatively related to the biosynthesis of unsaturated fatty acids, while Phaselicystis and Rubellimicrobium were mainly negatively related to the degradation of aminobenzoate, arginine and proline metabolism. The premature bolting of A. sinensis was related to the increase of nitrate nitrogen content in the rhizosphere soil, and the premature bolting will cause significant changes in the relative abundance of non-dominant bacteria and metabolites in the rhizosphere soil. The increase of nitrate nitrogen content may be related to the change of the relative abundance of Nitrosomonas, but further research is still needed.

Key words: Angelica sinensis bolting, rhizosphere soil, soil properties, bacterial community, metabolite

XIE Tian-peng, ZHANG Jia-ning, DONG Yong-jun, ZHANG Jian, JING Ming. Effect of Premature Bolting on the Rhizosphere Soil Microenvironment of Angelica sinensis[J]. Biotechnology Bulletin, 2023, 39(7): 206-218.

Figures/Tables 10

References 32

[1]	Wei WL, Zeng R, Gu CM, et al. Angelica sinensis in China-a review of botanical profile, ethnopharmacology, phytochemistry and chemical analysis[J]. J Ethnopharmacol, 2016, 190: 116-141. doi: 10.1016/j.jep.2016.05.023 URL
[2]	国家药典委员会. 中华人民共和国药典-一部: 2020年版[M]. 北京: 中国医药科技出版社, 2020: 139.
	Committee for the Pharmacopoeia of PR China. People's republic of China(PRC)pharmacopoeia-part I: 2020 edition[M]. Beijing: Chinese Medical Science and Technology Press, 2020: 139.
[3]	谢田朋, 柳娜, 王雅莉, 等. 不同产地当归品质的研究进展[J]. 中医药学报, 2020, 48(1): 72-75.
	Xie TP, Liu N, Wang YL, et al. Quality research progress of Angelica sinensis from different regions[J]. Acta Chin Med Pharmacol, 2020, 48(1): 72-75.
[4]	顾志荣, 师富贵, 王永琦, 等. 不同产地当归药材质量研究进展[J]. 甘肃中医学院学报, 2014, 31(5): 80-83.
	Gu ZR, Shi FG, Wang YQ, et al. Research progress on quality of Angelica sinensis from different habitats[J]. J Gansu Coll Tradit Chin Med, 2014, 31(5): 80-83.
[5]	冯慧敏, 李玥, 罗旭东, 等. 当归化学成分和药理作用研究进展及质量标志物的预测分析[J]. 中华中医药学刊, 2022, 40(4): 159-166.
	Feng HM, Li Y, Luo XD, et al. Research progress on chemical constituents and pharmacological effects of Danggui(Radix Angelica sinensis)and prediction analysis on its quality markers[J]. Chin Arch Tradit Chin Med, 2022, 40(4): 159-166.
[6]	Wang LY, Tang YP, Liu X, et al. Effects of ferulic acid on antioxidant activity in Angelica sinensis Radix, Chuanxiong Rhizoma, and their combination[J]. Chin J Nat Med, 2015, 13(6): 401-408.
[7]	Zhou WJ, Wang S, Hu Z, et al. Angelica sinensis polysaccharides promotes apoptosis in human breast cancer cells via CREB-regulated caspase-3 activation[J]. Biochem Biophys Res Commun, 2015, 467(3): 562-569. doi: 10.1016/j.bbrc.2015.09.145 URL
[8]	晋玲. 当归生产加工适宜技术[M]. 北京: 中国医药科技出版社, 2018: 1-14.
	Jin L. Suitable technology for production and processing of Angelica sinensis[M]. Beijing: China Pharmaceutical Science and Technology Press, 2018: 1-14.
[9]	王文丽, 李娟, 赵旭. 生物有机肥对连作当归根际土壤细菌群落结构和根腐病的影响[J]. 应用生态学报, 2019, 30(8): 2813-2821. doi: 10.13287/j.1001-9332.201908.030
	Wang WL, Li J, Zhao X. Effects of biological organic fertilizer on rhisosphere soil bacteria community and root rot diseases of continuous cropping Angelica sinensis[J]. Chin J Appl Ecol, 2019, 30(8): 2813-2821.
[10]	谢田朋, 柳娜, 刘越敏, 等. 化肥减量配施中药源植物生长调节剂对当归质量和根际土壤细菌群落的影响[J]. 生物技术通报, 2022, 38(3): 79-91. doi: 10.13560/j.cnki.biotech.bull.1985.2021-0467
	Xie TP, Liu N, Liu YM, et al. Effects of chemical fertilizer reduction and application of plant growth regulators from traditional Chinese medicine on the quality and its bacterial community in rhizosphere soil of Angelica sinensis[J]. Biotechnol Bull, 2022, 38(3): 79-91.
[11]	栗孟飞, 康天兰, 晋玲, 等. 当归抽薹开花及其调控途径研究进展[J]. 中草药, 2020, 51(22): 5894-5899.
	Li MF, Kang TL, Jin L, et al. Research progress on bolting and flowering of Angelica sinensis and regulation pathways[J]. Chin Tradit Herb Drugs, 2020, 51(22): 5894-5899.
[12]	Liu XX, Luo MM, Li MF, et al. Depicting precise temperature and duration of vernalization and inhibiting early bolting and flowering of Angelica sinensis by freezing storage[J]. Front Plant Sci, 2022, 13: 853444. doi: 10.3389/fpls.2022.853444 URL
[13]	邱黛玉, 蔺海明, 陈垣, 等. 经纬度和海拔对当归成药期植株长势和早期抽薹的影响[J]. 草地学报, 2010, 18(6): 838-843. doi: 10.11733/j.issn.1007-0435.2010.06.016
	Qiu DY, Lin HM, Chen Y, et al. Effects of latitude, longitude and altitude on Angelica growth and early bolting in medicine formation period[J]. Acta Agrestia Sin, 2010, 18(6): 838-843.
[14]	邱黛玉, 蔺海明, 方子森, 等. 种苗大小对当归成药期早期抽薹和生理变化的影响[J]. 草业学报, 2010, 19(6): 100-105.
	Qiu DY, Lin HM, Fang ZS, et al. Effects of seedlings with different root diameters on Angelica sinensis early bolting and physiological changes during the medicine formation period[J]. Acta Prataculturae Sin, 2010, 19(6): 100-105.
[15]	武延安, 刘效瑞, 曹占凤, 等. 日光温室冬季育苗抑制当归早期抽薹的效应研究[J]. 中国中药杂志, 2010, 35(3): 283-287.
	Wu YA, Liu XR, Cao ZF, et al. Inhibition of winter seedling raising in green house of premature bolting in Angelica sinensis[J]. China J Chin Mater Med, 2010, 35(3): 283-287.
[16]	谢田朋, 张建, 柳娜, 等. 当归不同生长时期根际土壤酶活性及微生物群落结构变化[J]. 土壤通报, 2023, 54(1):138-150.
	Xie TP, Zhang J, Liu N, et al. Changes in rhizosphere soil enzymatic activities and microbial communities across growth stages of Angelica sinensis[J]. Chin J Soil Sci, 2023, 54(1):138-150.
[17]	Mohanram S, Kumar P. Rhizosphere microbiome: revisiting the synergy of plant-microbe interactions[J]. Ann Microbiol, 2019, 69(4): 307-320. doi: 10.1007/s13213-019-01448-9
[18]	Lu T, Ke MJ, Lavoie M, et al. Rhizosphere microorganisms can influence the timing of plant flowering[J]. Microbiome, 2018, 6(1): 231. doi: 10.1186/s40168-018-0615-0 pmid: 30587246
[19]	Singh BK, Bardgett RD, Smith P, et al. Microorganisms and climate change: terrestrial feedbacks and mitigation options[J]. Nat Rev Microbiol, 2010, 8(11): 779-790. doi: 10.1038/nrmicro2439 pmid: 20948551
[20]	鲁如坤. 土壤农业化学分析方法[M]. 北京: 中国农业科技出版社, 2000.
	Lu RK. Methods of soil agrochemical analysis[M]. Beijing: China Agriculture Scientech Press, 2000.
[21]	巩晓芳, 祝英, 彭轶楠, 等. 当归不同生长时期根际丛枝真菌分布及土壤养分和酶活性的动态变化[J]. 微生物学通报, 2017, 44(11): 2596-2605.
	Gong XF, Zhu Y, Peng YN, et al. Dynamics of arbuscular mycorrhizal fungi distributions, soil nutrients and enzyme activities in rhizosphere soil at different growth stages of Angelica sinensis[J]. Microbiol China, 2017, 44(11): 2596-2605.
[22]	张宵娟, 邓光华, 喻苏琴, 等. 氮对千日红试管苗生长和开花诱导的影响[J]. 草业学报, 2015, 24(1): 56-63. doi: 10.11686/cyxb20150108
	Zhang XJ, Deng GH, Yu SQ, et al. Effects of nitrogen on growth and flowering of test-tube plantlets of Gomphrena globosa[J]. Acta Prataculturae Sin, 2015, 24(1): 56-63.
[23]	Gao YY, Qi SD, Wang Y. Nitrate signaling and use efficiency in crops[J]. Plant Commun, 2022, 3(5): 100353. doi: 10.1016/j.xplc.2022.100353 URL
[24]	Fan K, Fan DM, Su Y, et al. Cs-miR156 is involved in the nitrogen form regulation of catechins accumulation in tea plant(Camellia sinensis L.)[J]. Plant Physiol Biochem, 2015, 97: 350-360. doi: 10.1016/j.plaphy.2015.10.026 URL
[25]	Bao YY, Dolfing J, Guo ZY, et al. Important ecophysiological roles of non-dominant Actinobacteria in plant residue decomposition, especially in less fertile soils[J]. Microbiome, 2021, 9(1): 84. doi: 10.1186/s40168-021-01032-x pmid: 33827695
[26]	Peng JJ, Oladele O, Song XT, et al. Opportunities and approaches for manipulating soil-plant microbiomes for effective crop nitrogen use in agroecosystems[J]. Front Agr Sci Eng, 2022, 9(3): 333-343.
[27]	蔡雨衡, 向斯, 程凯. 氨氮对3种吸附态亚硝化单胞菌的抑制动力学[J]. 微生物学通报, 2021, 48(11): 3996-4005.
	Cai YH, Xiang S, Cheng K. The NH₃-N inhibition kinetics of 3 Nitrosomonas at adhesive status[J]. Microbiol China, 2021, 48(11): 3996-4005.
[28]	Cheng N, Peng YJ, Kong YL, et al. Combined effects of biochar addition and nitrogen fertilizer reduction on the rhizosphere metabolomics of maize(Zea mays L.) seedlings[J]. Plant Soil, 2018, 433(1): 19-35. doi: 10.1007/s11104-018-3811-6
[29]	Song Y, Li X, Yao S, et al. Correlations between soil metabolomics and bacterial community structures in the pepper rhizosphere under plastic greenhouse cultivation[J]. Sci Total Environ, 2020, 728: 138439. doi: 10.1016/j.scitotenv.2020.138439 URL
[30]	Pang ZQ, Chen J, Wang TH, et al. Linking plant secondary metabolites and plant microbiomes: a review[J]. Front Plant Sci, 2021, 12: 621276. doi: 10.3389/fpls.2021.621276 URL
[31]	Garcia R, Müller R. The family phaselicystidaceae[M]// Rosenberg E, DeLong EF, Lory S, et al. The Prokaryotes. Berlin: The Springer-Verlag Press, 2014: 239-245.
[32]	赵文慧, 马垒, 徐基胜, 等. 秸秆与木本泥炭短期施用对潮土有机质及微生物群落组成和功能的影响[J]. 土壤学报, 2020, 57(1): 153-164.
	Zhao WH, Ma L, Xu JS, et al. Effect of application of straw and wood peat for a short period on soil organic matter and microbial community in composition and function in fluvo-aquic soil[J]. Acta Pedol Sin, 2020, 57(1): 153-164.

理化性质 Physico-chemical properties	抽薹当归 Bolting	未抽薹当归 Unbolting
pH	7.86±0.26a	7.75±0.08a
有机质 Organic matter /（g·kg^-1）	2.93±1.48a	3.62±2.05a
盐分Salinity /（mg·kg^-1）	7.44±0.18a	7.66±0.30a
全氮 Total nitrogen /（g·kg^-1）	1.73±0.01a	1.73±0.01a
硝态氮 Nitrate nitrogen /（mg·kg^-1）	53.17±9.33a	42.36±6.19b
氨态氮 Ammonia nitrogen /（mg·kg^-1）	25.75±5.39a	21.76±3.54a
全磷 Total P /（g·kg^-1）	3.50±0.01a	3.50±0.01a
有效磷 Available P /（mg·kg^-1）	39.17±5.62a	44.06±5.47a
全钾 Total K /（g·kg^-1）	3.64±0.03a	3.64±0.01a
有效钾 Available K /（mg·kg^-1）	165.58±27.97a	171.65±34.34a

理化性质 Physico-chemical properties	抽薹当归 Bolting	未抽薹当归 Unbolting
pH	7.86±0.26a	7.75±0.08a
有机质 Organic matter /（g·kg^-1）	2.93±1.48a	3.62±2.05a
盐分Salinity /（mg·kg^-1）	7.44±0.18a	7.66±0.30a
全氮 Total nitrogen /（g·kg^-1）	1.73±0.01a	1.73±0.01a
硝态氮 Nitrate nitrogen /（mg·kg^-1）	53.17±9.33a	42.36±6.19b
氨态氮 Ammonia nitrogen /（mg·kg^-1）	25.75±5.39a	21.76±3.54a
全磷 Total P /（g·kg^-1）	3.50±0.01a	3.50±0.01a
有效磷 Available P /（mg·kg^-1）	39.17±5.62a	44.06±5.47a
全钾 Total K /（g·kg^-1）	3.64±0.03a	3.64±0.01a
有效钾 Available K /（mg·kg^-1）	165.58±27.97a	171.65±34.34a

Alpha多样性 Alpha diversity	抽薹当归 Bolting	未抽薹当归 Unbolting
测序覆盖度 Goods coverage	0.99±0.00a	0.99±0.00a
物种数 Observed species	2597.08±377.98a	2742.98±140.21a
Chao1	3065.85±325.88a	3209.56±128.91a
Shannon	8.56±0.97a	9.09±0.18a
Simpson	0.98±0.01a	0.99±0.00a

Alpha多样性 Alpha diversity	抽薹当归 Bolting	未抽薹当归 Unbolting
测序覆盖度 Goods coverage	0.99±0.00a	0.99±0.00a
物种数 Observed species	2597.08±377.98a	2742.98±140.21a
Chao1	3065.85±325.88a	3209.56±128.91a
Shannon	8.56±0.97a	9.09±0.18a
Simpson	0.98±0.01a	0.99±0.00a

门 Phylum	相对丰度 Relative abundance/%		属 Genus	相对丰度 Relative abundance/%
门 Phylum	抽薹当归Bolting	未抽薹当归Unbolting	属 Genus	抽薹当归Bolting	未抽薹当归Unbolting
变形菌门Proteobacteria	51.55±9.19a	50.91±5.98a	鞘氨醇单胞菌属Sphingomonas	5.74±1.42a	6.04±1.47a
放线菌门Actinobacteriota	23.19±8.54a	221.26±5.02a	链霉菌属Streptomyces	6.96±5.07a	3.83±2.51a
拟杆菌门Bacteroidota	12.00±2.38a	12.07±0.69a	假黄单胞菌属Pseudoxanthomonas	3.74±4.75a	1.83±0.52a
芽单胞菌门Gemmatimonadota	5.65±2.58a	6.50±2.12a	原小单孢菌属Promicromonospora	1.62±0.81a	2.09±1.51a
厚壁菌门Firmicutes	2.59±1.20a	2.98±1.04a	MND1	1.63±0.77a	1.58±0.87a
黏球菌门Myxococcota	2.20±0.48a	3.02±0.92a	溶杆菌属Lysobacter	1.58±0.73a	1.54±0.26a
酸杆菌门Acidobacteriota	0.98±0.65a	1.11±0.46a	Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium	2.00±3.26a	1.07±0.70a
绿弯菌门Chloroflexi	0.37±0.17a	0.33±0.09a	Muribaculaceae	1.32±0.57a	1.69±0.58a
硝化螺旋菌门Nitrospirota	0.23±0.24a	0.37±0.32a	Nocardioides	1.51±0.69a	1.45±0.58a
蛭弧菌门Bdellovibrionota	0.25±0.13a	0.33±0.12a	Ellin6067	1.36±0.64a	1.41±0.49a
疣微菌门Verrucomicrobiota	0.21±0.10a	0.20±0.09a	大肠杆菌志贺菌属Escherichia-Shigella	1.17±0.45a	1.39±0.60a
脱硫菌门Desulfobacterota	0.15±0.06a	0.18±0.09a	纤维弧菌属Cellvibrio	2.22±4.62a	0.29±0.15a
髌骨菌门Patescibacteria	0.08±0.05a	0.09±0.05a	假单胞菌属Pseudomonas	1.42±0.89a	0.98±0.65a
弯曲杆菌门Campilobacterota	0.06±0.02a	0.07±0.03a	Sphingopyxis	1.46±1.45a	0.91±0.51a
迷踪菌门Elusimicrobiota	0.07±0.04a	0.06±0.04a	0319-7L14	1.16±0.49a	1.20±0.32a
其他Others	0.41±0.16a	0.54±0.14a	其他Others	65.10±9.97a	72.71±3.83a