磷脂酶D信号转导与植物耐盐研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2016.10.012

摘要/Abstract

摘要： 土壤盐害是一个全球性的生态问题,对生态环境和农业生产带来了巨大的负面影响。研究发现,植物磷脂酶D（Phospholipase D,PLD）是磷脂代谢和应答非生物胁迫的重要酶类;PLD具有不同的结构、生化和调节特性,产生信号分子磷脂酸（Phosphatidic acid,PA）并参与多种胁迫反应。总结了PLD及其产物PA调控植物耐盐的相关报道,探讨其感受、应答盐信号的分子机制,为研究植物应答高盐胁迫和农作物分子遗传改良提供相关参考。

关键词: 盐胁迫, 磷脂酶D, 磷脂酸, 信号转导

Abstract: Soil salinity is a global ecological problem,which has a great negative impact on ecological environment and agricultural production. Researches showed that Phospholipase D（PLD）was a key enzyme in catalyzing lipids and response to abiotic stresses. PLDs contained diverse structures,distinguishable biochemical and regulatory properties,which produced phosphatidic acid（PA）and participated in plant response to various stresses. This review summarized the current advances in regulating plant salt tolerance,and discussed the putative molecular mechanisms of PLD in sensing and response to salt stress,which might benefit the study of plant salt resistance and crop genetic enhancement.

Key words: salt tolerance, PLD, PA, signal transduction

王培培, 宋萍, 张群. 磷脂酶D信号转导与植物耐盐研究进展[J]. 生物技术通报, 2016, 32(10): 58-65.

WANG Pei-pei, SONG Ping, ZHANG Qun. Plant Phospholipase D Signaling Pathway in Response to Salt Stress[J]. Biotechnology Bulletin, 2016, 32(10): 58-65.

参考文献

[1] 钟秀丽, 崔德才, 李玉中. 磷脂酶D的细胞信号转导作用[J]. 植物生理与分子生物学学报, 2005, 31（5）：451-460.
[2] Wang XM. Phospholipase D in hormonal ands tress signaling[J]. Current Opinion in Plant Biology, 2002, 5：408-414.
[3] Hong YY, Zhao J, Guo L, et al. Plant phospholipases D and C and their diverse functions in stress responses[J]. Progress in Lipid Research, 2016, 62：55-74.
[4] Hanahan DJ, Chaikoff IL. A new phospholipide-splitting enzyme specific for the ester linkage between the nitrogenous base and the phosphoric acid grouping[J]. The Journal Biological Chemistry, 1947, 169：699-705.
[5] 闫旭宇, 李玉中, 李玲, 等. 植物中的磷脂酶D信号转导[J]. 植物生理学通讯, 2006, 42（6）：1183-1189.
[6] Wang X. Lipid signaling[J]. Current Opinion in Plant Biollgy, 2004, 7：1-8.
[7] Wang X, Devaiah SP, Zhang W, et al. Signaling functions of phosphatidic acid[J]. Progress in Lipid Research, 2006, 45：250-278.
[8] Qin C, Wang XM. The Arabidopsis phospholipase D family. Characterization of a calcium-independent and phosphatidylcholine-selective PLD ζ 1 with distinct regulatory domains[J]. Plant Physiology, 2002, 128：1057-1068.
[9] Koonin EV. A duplicated catalytic motif in a new superfamily of pho-sphohydrolases and phospholipid synthases that includes poxvirus envelope proteins[J]. Trends in Biochemical Sciences, 1996, 21：242-243.
[10] Zheng L, Shan J, Krishnamoorthi R, et al. Activation of plant phospholipase Dβ by phosphatidylinositol 4, 5-bisphosphate：characterization of binding site and mode of action[J]. Biochemistry, 2002, 41：4546-4553.
[11] Zhao J, Wang X. Arabidopsis phospholipase Dα1 interacts with the heterotrimeric G-protein α-subunit through a motif analogous to the DRY motif in G-protein-coupled receptors[J]. The Journal Biological Chemistry, 2004, 279：1794-1800.
[12] Wang C, Wang XM. A novel phospholipase D of Arabidopsis that is activated by oleic acid and associated with the plasma membrane[J]. Plant Physiology, 2001, 127：1102-1112.
[13] Hong Y, Devaiah SP, Bahn SC, et al. Phospholipase Dε and phosphatidic acid enhance Arabidopsis nitrogen signaling and growth[J]. The Plant Journal, 2009, 58：376-387.
[14] Zhao J, Devaiah SP, Wang C, et al. Arabidopsis phospholipase Dβ1 modulates defense responses to bacterial and fungal pathogens[J]. New Phytologist, 2013, 199：228-240.
[15] Pinosa F, Buhot N, Kwaaitaal M, et al. Arabidopsis phospholipase Dδ is involved in basal defense and nonhost resistance to powdery mildew fungi[J]. Plant Physiology, 2013, 163：896-906.
[16] Hong Y, Pan X, Welti R, et al. Phospholipase Dα3 is involved in the hyperosmotic response in Arabidopsis[J]. The Plant Cell, 2008, 20：803-816.
[17] Cruz-Ramírez A, Oropeza-Aburto A, Razo-Hernández F, et al. Phospholipase DZ2 plays an important role in extraplastidic galactolipid biosynthesis and phosphate recycling in Arabidopsis roots[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103：6765-6770.
[18] Li G, Xue HW. Arabidopsis PLD ζ 2 regulates vesicle trafficking and is required for auxin response[J]. The Plant Cell, 2007, 19：281-295.
[19] Zhao J, Wang C, Bedair M, et al. Suppression of phospholipase Dγs confers increased aluminum resistance in Arabidopsis thaliana[J]. PLoS One, 2011, 6：e28086.
[20] Ljung K, Ostin A, Lioussanne L, et al. Developmental regulation of indole-3-acetic acid turnover in Scots pine seedlings[J]. Plant Physiology, 2001, 125：464-475.
[21] Normanly J, Cohen JD, Fink GR. Arabidopsis thaliana auxotrophs reveal a tryptophan- independent biosynthetic pathway for indole-3-acetic acid[J]. Proceedings of the National Academy of Sciences of the United States of America, 1991, 90：10355-10359.
[22] 夏石头, 萧浪涛. 生长素极性运输的调控及其机制[J]. 植物生理学通讯, 2003, 39（3）：255-261.
[23] 刘士平, 王璐, 王继荣, 等. 高等植物的PIN家族[J]. 植物生理学通讯, 2009, 45（8）：833-841.
[24] Bainbridge K, Guyomarch S, Bayer E, et al. Auxin influx carriers stabilize phyllotactic patterning[J]. Genes Development, 2008, 22（6）：810-823.
[25] Peer WA, Blakeslee JJ, Yang H, et al. Seven things we think we know about auxin transport[J]. Molecular Plant, 2011, 4（3）：487-504.
[26] Feraru E, Friml J. PIN polar targeting[J]. Plant Physiology, 2008, 147：1553-1559.
[27] Zhao Y, Wang T, Zhang W, et al. SOS3 mediates lateral root development under low salt stress through regulation of auxin redistribution and maxima in Arabidopsis[J]. New Phytologist, 2011, 189：1122-1134.
[28] Li G, Xue HW. Arabidopsis PLD ζ 2 regulates vesicle trafficking and is required for auxin response[J]. The Plant Cell, 2007, 19：281-295.
[29] Galvan-Ampudia CS, Julkowska MJ, Darwish E, et al. Halotropism is a response of plant roots to avoid a saline environment[J]. Current Biology, 2013, 23：2044-2050.
[30] Gao HB, Chu YJ, Xue HW. Phosphatidic acid（PA）binds PP2AA1 to regulate PP2A activity and PIN1 polar localization[J]. Molecular Plant, 2013, 6：1692-1702.
[31] Xu ZS, Liu L, Ni ZY, et al. W55a encodes a novel protein kinase that is involved in multiple stress responses[J]. Journal of Integrative Plant Biology, 2009, 51（1）：58-66.
[32] 孙大业. 植物细胞信号转导研究进展[J]. 植物生理学通讯, 1996, 32（2）：81-91.
[33] Bayerr G, Stael S, Rocha AG, et al. Chloroplast-localized protein kinases：A step forward towards a complete inventory[J]. Journal of Experimental Botany, 2012, 63（4）：1713-1723.
[34] Menke FLH, Van Pelt JA, Pieterse CMJ, et al. Silencing of the mitogen-activated protein kinase MPK6 compromises disease resistance in Arabidopsis[J]. The Plant Cell, 2004, 16（4）：897-907.
[35] Ichimura K, Mizoguchi T, Yoshida R, et al. Various abiotic stresses rapidly activate Arabidopsis MAP kinases ATMPK4 and ATMPK6[J]. The Plant Journal, 2000, 24：655-665.
[36] Yu LJ, Nie JN, Cao CY, et, al. Phosphatidic acid mediates salt stress response by regulation of MPK6 in Arabidopsis thaliana[J]. New Phytologist, 2010, 188：762-773.
[37] Testerink C, Larsen PB, van der Does D, et al. Phosphatidic acid binds to and inhibits the activity of Arabidopsis CTR1[J]. Journal of Experimental Botany, 2007, 58：3905-3914.
[38] Wang YN, Wang T, Li KX, et al. Genetic analysis of involvement of ETR1 in plant response to salt and osmotic stress[J]. Plant Growth Regulation, 2008, 54：261-269.
[39] Lei G, Shen M, Li ZG, et al. EIN2 regulates salt stress response and interacts with a MA3 domain containing protein ECIP1 in Arabidopsis[J]. Plant Cell and Environment, 2011, 34：1678-1692.
[40] Anthony RG, Henriques R, Helfer A, et al. A protein kinase target of a PDK1 signalling pathway is involved in root hair growth in Arabidopsis[J]. EMBO Journal, 2004, 23：572-581.
[41] Zegzouti RG, Anthony N, Jahchan L, et al. Phosphorylation and activation of PINOID by the phospholipid signaling kinase 3-phosphoinositide-dependent protein kinase 1（PDK1）in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103：6404-6409.
[42] Galvan-Ampudia CS, Testerink C. Salt stress signals shape the plant root[J]. Current Opinion in Plant Biology, 2011, 13：296-302.
[43] Guo L, Mishra G, Taylor K, et al. Phosphatidic acid binds and stimulates Arabidopsis[J]. The Journal of Biological Chemistry, 2011, 286：13336-13345.
[44] Guo L, Mishra G. Connections between sphingosine kinase and Phospholipase D in the abscisic acid signaling pathway in Arabidopsis[J]. The Journal of Biological Chemistry, 2012, 287：8286-8296.
[45] Xiong LZ, Yang YN. Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase[J]. The Plant Cell, 2003, 15（3）：745 -759.
[46] Lee SK, Kim BG, Kwon TR, et al. Overexpression of the mitogen-activated protein kinase gene OsMAPK33 enhances sensitivity to salt stress in rice（Oryza sativa L. ）[J]. Journal of Biosciences, 2011, 36（1）：139-151.
[47] Sun X, Yang S, Sun M, et al. A novel Glycine soja cysteine proteinase inhibitor GsCPI14, interacting with the calcium/calmodulin-binding receptor-like kinase GsCBRLK, regulated plant tolerance to alkali stress[J]. Plant Molecular Biology, 2014, 85：33-48.
[48] Dammann C, Ichida A, Hong B, et al. Subcellular targeting of nine calcium-dependent protein kinase isoforms from Arabidopsis[J]. Plant Physiology, 2003, 132（4）：1840-1848.
[49] Campo S, Baldrich P, Messeguer J, et al. Overexpression of a calcium-dependent protein kinase confers salt and drought tolerance in rice by preventing membrane lipid peroxidation[J]. Plant Physiology, 2014, 165（2）：688-704.
[50] Saijo Y, Hata S, Kyozuka J, et al. Over-expression of a single Ca 2+ -dependent protein kinase confers both cold and salt / drought tolerance onrice plants[J]. The Plant Journal, 2000, 23（3）：319-327.
[51] Ouyang SQ, Liu YF, Liu P, et al. Receptor-like kinase OsSIK1 improves drought and salt stress tolerance in rice（Oryza sativa）plants[J]. The Plant Journal, 2010, 62（2）：316-329.
[52] Chen LJ, Wuriyanghan H, Zhang YQ, et al. An s-domain receptor-like kinase, OsSIK2, confers abiotic stress tolerance and delays dark-induced leaf senescence in rice[J]. Plant Physiology, 2013, 163（4）：1752-1765.
[53] Zhang Q, Lin F, Mao TL, et al. Phosphatidic acid regulates microtubule organization by interacting with MAP65-1 in response to salt stress in Arabidopsis[J]. The Plant Cell, 2012, 24：4555-4576.
[54] 时兰春, 王益川, 王伯初. 植物细胞骨架与细胞生长[J]. 植物生理学通讯, 2007, 43：1175-1181.
[55] Dhonukshe P, Laxalt AM, Goedhart J, et al. Phospholipase D activation correlates with microtubule reorganization in living plant cells[J]. The Plant Cell, 2003, 15：2666-2679.
[56] Wang C, Li J, Yuan M. Salt tolerance requires cortical microtubule reorganization in Arabidopsis[J]. Plant and Cell Physiology, 2007, 48：1534-1547.
[57] Mao G, Chan J, Calder Q, et al. Modulated taigeting of GFP-AtMAP65-1 to central spindle microtubules during division[J]. The Plant Journal, 2005, 43：469-478.
[58] Komis G, Quader H, Galatis B, et al. Macrotubule-dependent protoplast volume regulation in plasmolysed root-tip cells of Triticum turgidum：involvement of phospholipase D[J]. New Phytologist, 2006, 171：737-750.
[59] Beck M, Komis G, Muller J, et al. Arabidopsis homologs of nucleus-and phragmoplast-localized kinase 2 and 3 and mitogen-activated protein kinase 4 are essential for microtubule organization[J]. The Plant Cell, 2010, 22：755-771.
[60] Huang S, Blanchoin L, Kovar DR, et al. Arabidopsis capping protein（AtCP）is a heterodimer that regulates assembly at the barbed ends of actin filaments[J]. The Journal of Biological Chemistry, 2003, 278：44832-44842.
[61] Huang S, Gao L, Blanchoin L, et al. Heterodimeric capping protein from Arabidopsis is regulated by phosphatidic acid[J]. Molecular Biology of the Cell, 2006, 17：1946-1958.
[62] Li J, Henty-Ridilla JL, Huang S, et al. Capping protein modulates the dynamic behavior of actin filaments in response to phosphatidic acid in Arabidopsis[J]. The Plant Cell, 2012, 24：3742-3754.
[63] Wang J, Qian D, Fan T, et al. Arabidopsis actin capping protein（AtCP）subunits have different expression patterns, and downregulation of AtCPB confers increased thermotolerance of Arabidopsis after heat shock stress[J]. Plant Science, 2012：110-119.
[64] Gardiner JC, Harper JD, Weerakoon ND, et al. A 90-kD phospholipase D from tobacco binds to microtubules and the plasma membrane[J]. The Plant Cell, 2001, 13：2143-2158.
[65] Zhang W, Wang C, Qin C, et al. The oleate-stimulated phospholipase D, PLDδ, and phosphatidic acid decrease H 2 O 2 -induced cell death in Arabidopsis[J]. The Plant Cell, 2003, 15：2285-2295.
[66] Zhang Q, Zhang W. Regulation of developmental and environmental signaling by interaction between microtubules and membranes in plant cells[J]. Protein Cell, 2016, 7（2）：81-88.
[67] Pleskot R, Potocký M, Pejchar P, et al. Mutual regulation of plant phospholipase D and the actin cytoskeleton[J]. The Plant Journal, 2010, 62：494-507.
[68] Zhang Y, Zhu H, Zhang Q, et al. Phospholipase dalpha1 and phosphatidic acid regulate NADPH oxidase activity and production of reactive oxygen species in ABA-mediated stomatal closure in Arabidopsis[J]. The Plant Cell, 2009, 21：2357-2377.
[69] Guo L, Devaiah SP, Narasimhan R, et al. Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenases interact with Phospholipase Dδ to transduce hydrogen peroxide signals in the Arabidopsis response to stress[J]. The Plant Cell, 2012, 24：2200-2212.
[70] Hirayama T, Ohto C, Mizoguchi T, et al. A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana[J]. Proceedings of the National Academy of Sciences of the United States of America, 1995, 92（9）：3903-3907.
[71] Sanchez JP, Chua NH. Arabidopsis PLC1 is required for secondary responses to abscisic acid signals[J]. The Plant Cell, 2001, 13：1143-1154.
[72] Xu X, Cao Z, Liu G, et al. Cloning and expression of AtPLC6, a gene encoding a phosphatidylinositol-specific phospholipase C in Arabidopsis thaliana[J]. Chinese Science Bulletin, 2004, 49（4）：567-573.
[73] Yun JK, Kim JE, Lee JH, et al. The Vr-PLC3 gem encodes a putative plasma membrane-localized phosphoinositide-specific phospholipase C whose expression is induced by abiotic stress in mung bean（Vigna radiata L.）[J]. FEBS Letters, 2004, 556（1-3）：127-136.
[74] 王春荣. 磷脂酶C基因的过表达对玉米转基因植株抗逆性的影响[D].济南：山东大学, 2008.
[75] 梵文菊.转ZmPLC1基因及聚合ZmPLCa/betA基因棉花耐盐性的研究[D].济南：山东大学, 2013.
[76] 王法微.水稻磷脂酶C调节耐盐性以及磷脂酶D参与种子老化的研究[D].南京：南京农业大学, 2011.