青杄PwNAC42基因的克隆及表达模式分析

doi:10.13560/j.cnki.biotech.bull.1985.2017-0924

生物技术通报 ›› 2018, Vol. 34 ›› Issue (3): 113-120.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0924

青杄PwNAC42基因的克隆及表达模式分析

袁义杭, 张鹤华, 游韩莉, 张凌云

北京林业大学省部共建森林培育与保护教育部重点实验室，北京 100083

收稿日期:2017-10-31 出版日期:2018-03-20 发布日期:2018-04-10
作者简介:袁义杭，男，硕士研究生，研究方向植物抗逆基因克隆和功能；E-mail283771539@qq.com
基金资助:
转基因生物新品种培育重大专项（2016ZX08009003-002）

Cloning and Expression Analysis of PwNAC42 in Picea wilsonii

YUAN Yi-hang, ZHANG He-hua, YOU Han-li, ZHANG Ling-yun

Key Laboratory of Forestry Silviculture and Conservation of the Ministry of Education，Beijing Forestry University，Beijing 100083

Received:2017-10-31 Published:2018-03-20 Online:2018-04-10

摘要/Abstract

摘要： NAC转录因子是植物中最大的转录因子家族之一，广泛参与了植物的生长发育过程，并在植物响应盐害、干旱、冷害和脱落酸等多种非生物胁迫的过程中发挥重要作用。通过青杄转录组数据获得PwNAC42基因的cDNA序列，对其蛋白序列进行生物信息学预测，通过实时荧光定量PCR检测PwNAC42基因在青杄各个组织及非生物逆境胁迫下的表达水平。生物信息学分析结果显示：PwNAC42基因cDNA全长共1 749 bp，其中编码区1 140 bp，共编码379个氨基酸；蛋白理论分子质量为42.92 kD，理论等电点为6.53，有丝氨酸、苏氨酸和酪氨酸磷酸化位点，不存在信号肽结构域，为非跨膜的亲水蛋白。组织特异性表达结果显示，PwNAC42在青杄球果中表达量最高。逆境胁迫实验表明PwNAC42的表达量在NaCl、干旱、4℃以及脱落酸处理下产生显著的变化。因此推测PwNAC42在青杄球果发育及青杄对逆境胁迫的响应过程中发挥作用。

关键词: 青杄, NAC转录因子, PwNAC42, 胁迫响应

Abstract: As one of the largest plant transcription factors，NAC is widely involved in growth and development，as well as the responses of plants to abiotic stresses. In this study，PwNAC42 cDNA was obtained by Picea wilsonii transcriptome data，its protein sequence was analyzed by bioinformatics，and the expression of PwNAC42 was detected by real-time qPCR. The results showed that the full-length of PwNAC42 cDNA was 1749 bp，including 1140 bp of ORF that encoded 379 amino acids. The molecular weight of PwNAC42 was 42.92 kD and the pI value was 6.53. PwNAC42 had phosphorylation sites of serine，threonine and tyrosine. It was a hydrophilic protein that had no signal peptide domain and transmembrane domain. RT-qPCR results showed that PwNAC42 mainly expressed in cones. At the same time，the expression of PwNAC42 under ABA，NaCl，drought and low temperature treatment changed significantly. Therefore，P. wilsonii PwNAC42 may play a role in cone development and response to abiotic stress.

Key words: Picea wilsonii, NAC transcription factor, PwNAC42, stress response

袁义杭, 张鹤华, 游韩莉, 张凌云. 青杄PwNAC42基因的克隆及表达模式分析[J]. 生物技术通报, 2018, 34(3): 113-120.

YUAN Yi-hang, ZHANG He-hua, YOU Han-li, ZHANG Ling-yun. Cloning and Expression Analysis of PwNAC42 in Picea wilsonii[J]. Biotechnology Bulletin, 2018, 34(3): 113-120.

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青杄PwNAC42基因的克隆及表达模式分析

Cloning and Expression Analysis of PwNAC42 in Picea wilsonii

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