miR159-GAMYB途径调控植物生长发育的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2021-0028

摘要/Abstract

摘要：

miR159（microRNA159）是植物中一类古老而又保守的microRNA,其靶基因主要是一类编码R2R3 MYB转录因子的GAMYB-like基因。miR159-GAMYB途径高度保守,miR159通过转录后调控GAMYB在植物营养生长、开花诱导、雄性生殖、花器官发育、植物育性、果实发育、种子萌发和胁迫响应中发挥着重要作用。主要探讨了近些年来miR159-GAMYB途径对植物生长发育的影响,为今后相关研究提供参考。

关键词: miR159, GAMYB, 营养生长, 花药, 胁迫响应, 赤霉素

Abstract:

miR159（microRNA159）is an ancient and conserved microRNA in plants,which targets a class of regulatory genes called GAMYB-like that encode R2R3 MYB transcription factors. miR159-GAMYB pathway is highly conserved,miR159 plays a critical role in vegetative growth,flowering induction,male reproduction,floral organ development,plant fertility,fruit development,seed sprouting and stress response by the post-transcriptional regulation of GAMYB. The article mainly discusses the influence of miR159-GAMYB pathway regulating plants growth and development,which provides a reference for future related researches.

Key words: miR159, GAMYB, vegetative growth, anther, stress response, gibberellins

黎猛, 陈跃, 胡凤荣. miR159-GAMYB途径调控植物生长发育的研究进展[J]. 生物技术通报, 2021, 37(9): 234-247.

LI Meng, CHEN Yue, HU Feng-rong. Research Progress in miR159-GAMYB Regulating Plants Growth and Development[J]. Biotechnology Bulletin, 2021, 37(9): 234-247.

图/表 2

参考文献 81

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物种Species	技术方法Approach	表型Phenotype	参考文献Reference
拟南芥Arabidopsis thaliana	miR159a过表达（Columbia型）	雄性不育	[57]
	miR159a过表达（Landsberg型）	雄性不育、开花延迟	[59]
	T-DNA mir159ab突变	植株矮小、生长延缓、叶子卷曲、角果和种子变小、种子畸形、主根变长、开花延迟	[13-14,19,34]
	T-DNA mir159abc突变	受精异常、植株矮小、生长延缓、叶子卷曲、角果和种子变小	[14,40]
	T-DNA mir159c突变	无	[14]
	MIM159模拟功能缺失	植株矮小、生长延缓、叶子卷曲、主根变长,花萼、花瓣、花药发育异常	[24-26]
	mMYB33过表达	植株矮小、生长延缓、叶子卷曲	[13,23]
	T-DNA myb33、myb65突变	雄性不育、二倍体花粉	[23,36]
	T-DNA myb81突变	花粉异常	[37]
	T-DNA myb97、myb101、myb120突变	种子糊粉异常、雄性不育	[19,38-39]
水稻Oryza sativa	miR159过表达	叶鞘畸形、节间伸长异常、雄性不育、花药变白皱缩、花变小、雌蕊棕色皱缩	[30]
	STTM159模拟功能缺失	植株矮小,叶、圆锥花序尺寸变小、节间缩短、种子变小	[27]
	MIM159模拟功能缺失	植株矮小、生长延缓、剑叶短小	[21]
	gamyb转座子插入突变	叶鞘畸形、节间伸长异常、雄性不育花药变白皱缩、花变小	[31]
番茄Solanum lycopersicum	miR159过表达	果实早熟、单性结实、植株变矮、花变小	[29]
	MIM159模拟功能缺失	植株变高、种子变多	[29]
	RNAi沉默SlMYB33	花粉成熟异常、低育性、果实变小、开花延迟	[43]
	SlMYB33过表达	果实增大、开花延迟	[55]
大岩桐Sinningia speciosa	miR159a过表达	开花延迟	[32]
大岩桐Sinningia speciosa	MIM159模拟功能缺失	开花提前、花萼花瓣发育异常	[32]
烟草Nicotiana tabacum	MIM159模拟功能缺失	植株矮小、生长延缓、叶子变小变皱、雄蕊变短、花色变白、花型变小、开花延迟	[21]
烟草Nicotiana tabacum	mNtGAMYB2过表达	植株矮小、生长延缓、叶子变小变皱、雄蕊变短、花色变白、花型变小	[21]
大麦Hordeum vulgare	HvGAMYB过表达	雄性不育	[47]
草莓Fragaria × ananassa	RNAi沉默FaGAMYB	花托成熟中止、花托颜色受抑制	[54]
黄瓜Cucumis sativus	RNAi沉默CsGAMYB	降低雄花和雌花的比例	[46]
白菜Brassica campestris	miR159a过表达	雄性不育	[44-45]
青菜Brassica rapa ssp. Chinensis	VIGS沉默BcMYB101	叶片上卷	[33]

物种Species	技术方法Approach	表型Phenotype	参考文献Reference
拟南芥Arabidopsis thaliana	miR159a过表达（Columbia型）	雄性不育	[57]
	miR159a过表达（Landsberg型）	雄性不育、开花延迟	[59]
	T-DNA mir159ab突变	植株矮小、生长延缓、叶子卷曲、角果和种子变小、种子畸形、主根变长、开花延迟	[13-14,19,34]
	T-DNA mir159abc突变	受精异常、植株矮小、生长延缓、叶子卷曲、角果和种子变小	[14,40]
	T-DNA mir159c突变	无	[14]
	MIM159模拟功能缺失	植株矮小、生长延缓、叶子卷曲、主根变长,花萼、花瓣、花药发育异常	[24-26]
	mMYB33过表达	植株矮小、生长延缓、叶子卷曲	[13,23]
	T-DNA myb33、myb65突变	雄性不育、二倍体花粉	[23,36]
	T-DNA myb81突变	花粉异常	[37]
	T-DNA myb97、myb101、myb120突变	种子糊粉异常、雄性不育	[19,38-39]
水稻Oryza sativa	miR159过表达	叶鞘畸形、节间伸长异常、雄性不育、花药变白皱缩、花变小、雌蕊棕色皱缩	[30]
	STTM159模拟功能缺失	植株矮小,叶、圆锥花序尺寸变小、节间缩短、种子变小	[27]
	MIM159模拟功能缺失	植株矮小、生长延缓、剑叶短小	[21]
	gamyb转座子插入突变	叶鞘畸形、节间伸长异常、雄性不育花药变白皱缩、花变小	[31]
番茄Solanum lycopersicum	miR159过表达	果实早熟、单性结实、植株变矮、花变小	[29]
	MIM159模拟功能缺失	植株变高、种子变多	[29]
	RNAi沉默SlMYB33	花粉成熟异常、低育性、果实变小、开花延迟	[43]
	SlMYB33过表达	果实增大、开花延迟	[55]
大岩桐Sinningia speciosa	miR159a过表达	开花延迟	[32]
大岩桐Sinningia speciosa	MIM159模拟功能缺失	开花提前、花萼花瓣发育异常	[32]
烟草Nicotiana tabacum	MIM159模拟功能缺失	植株矮小、生长延缓、叶子变小变皱、雄蕊变短、花色变白、花型变小、开花延迟	[21]
烟草Nicotiana tabacum	mNtGAMYB2过表达	植株矮小、生长延缓、叶子变小变皱、雄蕊变短、花色变白、花型变小	[21]
大麦Hordeum vulgare	HvGAMYB过表达	雄性不育	[47]
草莓Fragaria × ananassa	RNAi沉默FaGAMYB	花托成熟中止、花托颜色受抑制	[54]
黄瓜Cucumis sativus	RNAi沉默CsGAMYB	降低雄花和雌花的比例	[46]
白菜Brassica campestris	miR159a过表达	雄性不育	[44-45]
青菜Brassica rapa ssp. Chinensis	VIGS沉默BcMYB101	叶片上卷	[33]