微藻油脂含量的高效测定方法

doi:10.13560/j.cnki.biotech.bull.1985.2019-0364

摘要/Abstract

摘要： 随着化石能源的逐年消耗以及人们环境保护意识的不断增强,微藻作为一种可持续的绿色能源近年来逐渐成为世界各国科学工作者研究的热点。选育高油脂含量微藻是发展微藻生物能源的重要一环,而高效快速的油脂测定方法则是选育过程中的关键技术之一。概述了几种高效、准确、简便的微藻油脂含量测定方法的基本原理和研究现状,主要包括尼罗红荧光光谱法、BODIPY 505/515荧光染色法、铜试剂法、苏丹黑B染色法、傅里叶变换红外光谱法、时域核磁共振法、可见/近红外光谱法等,并对这些方法各自的优缺点进行了比较,以期为微藻油脂含量测定提供一定的参考,同时为微藻油脂研究和生产实践提供理论依据。

关键词: 微藻, 油脂测定, 高效测定方法, 生物能源

Abstract: Due to the petroleum reserves diminishing and the concerns of global environmental protection rising,microalgae,as a sustainable and renewable energy source,has been attracted wide attention in recent years. The breeding of microalgae with high oil content is an important step in the development of microalgae bioenergy,and the efficient and rapid determination method of lipids is one of the key technologies in the breeding process. This paper reviews the basic principles and research status of several efficient,accurate and convenient determination methods of lipids,such as Nile red fluorescence spectrometry,BODIPY 505/515 fluorescence spectrometry,copper reagent method,Sudan black B staining method,Fourier transform infrared spectrometry,time-domain nuclear magnetic resonance method,and vis/near-infrared spectrometry. Furthermore,the advantages and methodological limitations of these methods are also compared,aiming at providing a grateful reference for determination of microalgae lipid and concurrently some scientific know-how for subsequent research on the production of microalgae oil.

Key words: microalgae, determination of lipid, efficient method, bioenergy

李志, 周秋香, 黎秋玲, 刘孟荧, 周智友, 李汉广. 微藻油脂含量的高效测定方法[J]. 生物技术通报, 2019, 35(12): 189-195.

LI Zhi, ZHOU Qiu-xiang, LI Qiu-ling, LIU Meng-ying, ZHOU Zhi-you, LI Han-guang. Overview on Efficient Methods for the Determination of Microalgae Lipid Content[J]. Biotechnology Bulletin, 2019, 35(12): 189-195.

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