小麦蛋白质二硫键异构酶基因家族的鉴定与表达

doi:10.13560/j.cnki.biotech.bull.1985.2024-0718

生物技术通报 ›› 2025, Vol. 41 ›› Issue (2): 85-96.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0718

小麦蛋白质二硫键异构酶基因家族的鉴定与表达

葛仕杰¹^,²(), 刘怡德¹, 张华东¹, 宁强¹, 朱展望¹, 王书平²(), 刘易科¹^,²()

^1.湖北省农业科学院粮食作物研究所农业农村部作物分子育种重点实验室粮食作物种质创新与遗传改良湖北省重点实验室，武汉 430064
^2.长江大学农学院农业农村部长江中游作物绿色高效生产重点实验室（部省共建），荆州 434025

收稿日期:2024-07-26 出版日期:2025-02-26 发布日期:2025-02-28
通讯作者: 刘易科，男，副研究员，研究方向：小麦抗赤霉病分子育种；E-mail: hbliuyk@foxmail.com
王书平，男，副教授，研究方向：小麦发育生物学；E-mail: wangshuping2003@126.com
作者简介:葛仕杰，男，硕士研究生，研究方向：小麦抗赤霉病分子育种；E-mail: gsj13797691553@163.com
基金资助:
国家自然科学基金项目(32272170);武汉市知识创新专项(2022020801010341)

Identification and Expression Analysis of Protein Disulfide Isomerase Gene Family in Wheat

GE Shi-jie¹^,²(), LIU Yi-de¹, ZHANG Hua-dong¹, NING Qiang¹, ZHU Zhan-wang¹, WANG Shu-ping²(), LIU Yi-ke¹^,²()

^1.Institute of Food Crops, Hubei Academy of Agricultural Sciences, Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan 430064
^2.MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtza River (Co-Construction by Ministry and Province), College of Agronomy, Yangtze University, Jingzhou 434025

Received:2024-07-26 Published:2025-02-26 Online:2025-02-28

摘要/Abstract

摘要：

目的通过对小麦蛋白质二硫键异构酶PDIL基因家族成员进行鉴定及表达分析，分析其在小麦不同器官和不同发育时期以及响应生物和非生物胁迫中的表达模式，为明确小麦PDIL基因家族功能奠定基础。方法以模式植物拟南芥和禾本科作物大麦PDIL基因家族蛋白序列为诱饵，在基因组数据库对小麦PDIL家族基因进行全基因组鉴定，利用生物信息学方法对其基因的理化性质、系统进化、染色体位置及基因结构进行分析，并分析PDILs基因在小麦生长发育、生物及非生物逆境的表达模式。结果鉴定得到31个小麦PDIL基因家族成员均为亲水蛋白，其中26个为稳定蛋白；亚细胞定位预测表明，小麦PDIL蛋白主要分布在细胞质和叶绿体中，在内质网和细胞核中也有分布；小麦PDIL基因家族可分为7个进化分支，同一分支基因结构和保守基序相似或相同，非均匀分布在19个染色体上；小麦PDIL基因家族成员含有多种响应逆境胁迫以及与水杨酸（SA）、茉莉酸甲酯（MeJA）、赤霉素（GA）、脱落酸（ABA）和生长素（IAA）等5类植物激素相关的顺式作用元件，部分基因具有与昼夜节律控制以及种子特性调控相关的元件；转录组数据表明，TaPDIL4-1A、TaPDIL4-1B和TaPDIL4-1D在不同生长时期组织都有较高的表达量；转录组分析和RT-PCR结果显示，TaPDIL4-1B在禾谷镰刀菌侵染后相对表达量升高，TaPDIL1-4A在干旱胁迫下表达量提高。结论 31个小麦PDIL基因家族成员在进化过程中具有保守性，不仅参与小麦品质形成，还广泛参与生物和非生物逆境调控，其中TaPDIL4-1B可能参与小麦赤霉病抗性的调控，TaPDIL1-4A可能参与小麦干旱胁迫的应答。

关键词: 小麦, PDIL基因家族, 表达分析, 生物胁迫, 非生物胁迫

Abstract:

Objective The objective of this study is to perform the genome-wide identification of the wheat PDIL (Protein Disulfide Isomerase) gene family members, and to analyze their expression patterns in different organs and developmental stages of wheat, as well as in response to biotic and abiotic stress, which lays the foundation for clarifying the function of wheat PDIL gene family. Method Using protein sequences of PDIL genefamily of Arabidopsis thaliana and Gramineae crop barley as bait, genome-wide identification of wheat PDIL family genes was conducted in the genome database. Then the physicochemical properties, phylogenetics, chromosome position and gene structure were analyzed using bioinformatics methods. And the expression pattern of PDILs genes in wheat growth and development, biotic and abiotic adversity were analyzed. Result The 31 wheat PDIL gene family members were identified as hydrophilic proteins, of which 26 were stable proteins. Subcellular localization prediction indicated that wheat PDIL proteins were mainly distributed in the cytoplasm and chloroplasts, as well as in the endoplasmic reticulum and nucleus. The wheat PDIL gene family could be divided into 7 evolutionary branches, and the gene structure and conserved motif of the same branch were similar or identical, and they were not uniformly distributed on 19 chromosomes. The promoters of wheat PDIL gene family members contained multiple cis-acting elements that responded to stress and associated with five types of plant hormones, including salicylic acid (SA), methyl jasmonate (MeJA), gibberellin (GA), abscisic acid (ABA), and auxin (IAA), and a few genes also contained elements related to circadian rhythm control and seed trait regulation. Transcriptome data showed that TaPDIL4-1A, TaPDIL4-1B, and TaPDIL4-1D genes had high expressions in tissues at different growth stages. Transcriptome analysis and RT-PCR results showed that the relative expression of TaPDIL4-1B increased after infection with Fusarium graminearum, and the expression of TaPDIL1-4A gene increased under drought stress. Conclusion The 31 members of the wheat PDIL gene family have conservation during evolution, not only participating in wheat quality formation, but also widely involved in biotic and abiotic stress regulation. Among them, the TaPDIL4-1B gene might be involved in the regulation of wheat FHB resistance, while the TaPDIL1-4A gene might be involved in the response to wheat drought stress.

Key words: wheat, PDIL gene family, expression analysis, biotic stress, abiotic stress

葛仕杰, 刘怡德, 张华东, 宁强, 朱展望, 王书平, 刘易科. 小麦蛋白质二硫键异构酶基因家族的鉴定与表达[J]. 生物技术通报, 2025, 41(2): 85-96.

GE Shi-jie, LIU Yi-de, ZHANG Hua-dong, NING Qiang, ZHU Zhan-wang, WANG Shu-ping, LIU Yi-ke. Identification and Expression Analysis of Protein Disulfide Isomerase Gene Family in Wheat[J]. Biotechnology Bulletin, 2025, 41(2): 85-96.

图/表 9

参考文献 34

1	张政荣. 拟南芥蛋白质二硫键异构酶(AtPDIL1)酶活性及其抗逆功能的研究 [D]. 泰安: 山东农业大学, 2017.
	Zhang ZR. Study on the activity of protein disulfide isomerase (AtPDIL1) in Arabidopsis thaliana and its stress resistance function [D]. Tai’an: Shandong Agricultural University, 2017.
2	Wilkinson B, Gilbert HF. Protein disulfide isomerase [J]. Biochim Biophys Acta, 2004, 1699(1/2): 35-44.
3	Goldberger RF, Epstein CJ, Anfinsen CB. Acceleration of reactivation of reduced bovine pancreatic ribonuclease by a microsomal system from rat liver [J]. J Biol Chem, 1963, 238: 628-635.
4	时丽洁, 蒋枞璁, 王方梅, 等. 大麦蛋白质二硫键异构酶基因家族的鉴定与表达分析 [J]. 作物学报, 2019, 45(9): 1365-1374.
	Shi LJ, Jiang CC, Wang FM, et al. Genome-wide characterization and transcriptional analysis of the protein disulfide isomerase-like genes in barley (Hordeum vulgare) [J]. Acta Agron Sin, 2019, 45(9): 1365-1374.
5	Holmgren A. Thioredoxin and glutaredoxin systems [J]. J Biol Chem, 1989, 264(24): 13963-13966.
6	Matsui M, Oshima M, Oshima H, et al. Early embryonic lethality caused by targeted disruption of the mouse thioredoxin gene [J]. Dev Biol, 1996, 178(1): 179-185.
7	Gruber CW, Cemazar M, Heras B, et al. Protein disulfide isomerase: the structure of oxidative folding [J]. Trends Biochem Sci, 2006, 31(8): 455-464.
8	Darby NJ, Kemmink J, Creighton TE. Identifying and characterizing a structural domain of protein disulfide isomerase [J]. Biochemistry, 1996, 35(32): 10517-10528.
9	Kemmink J, Darby NJ, Dijkstra K, et al. The folding catalyst protein disulfide isomerase is constructed of active and inactive thioredoxin modules [J]. Curr Biol, 1997, 7(4): 239-245.
10	Klappa P, Ruddock LW, Darby NJ, et al. The b′ domain provides the principal peptide-binding site of protein disulfide isomerase but all domains contribute to binding of misfolded proteins [J]. EMBO J, 1998, 17(4): 927-935.
11	Hatahet F, Ruddock LW. Protein disulfide isomerase: a critical evaluation of its function in disulfide bond formation [J]. Antioxid Redox Signal, 2009, 11(11): 2807-2850.
12	Lu DP, Christopher DA. Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana [J]. Mol Genet Genomics, 2008, 280(3): 199-210.
13	Houston NL, Fan CZ, Xiang JQY, et al. Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins [J]. Plant Physiol, 2005, 137(2): 762-778.
14	Shorrosh BS, Dixon RA. Molecular characterization and expression of an alfalfa protein with sequence similarity to mammalian ERp72, a glucose-regulated endoplasmic reticulum protein containing active site sequences of protein disulphide isomerase [J]. Plant J, 1992, 2(1): 51-58.
15	刘颖慧, 王秀堂, 石云素, 等. 玉米蛋白质二硫键异构酶(PDI)基因的特征和表达 [J]. 中国生物化学与分子生物学报, 2009, 25(3): 229-234.
	Liu YH, Wang XT, Shi YS, et al. Expression and characterization of a protein disulfide isomerases in maize (Zea mays L.) [J]. Chin J Biochem Mol Biol, 2009, 25(3): 229-234.
16	Kimura S, Higashino Y, Kitao Y, et al. Expression and characterization of protein disulfide isomerase family proteins in bread wheat [J]. BMC Plant Biol, 2015, 15: 73.
17	Meiri E, Levitan A, Guo F, et al. Characterization of three PDI-like genes in Physcomitrella patens and construction of knock-out mutants [J]. Mol Genet Genomics, 2002, 267(2): 231-240.
18	Kayum MA, Park JI, Nath UK, et al. Genome-wide characterization and expression profiling of PDI family gene reveals function as abiotic and biotic stress tolerance in Chinese cabbage (Brassica rapa ssp. pekinensis) [J]. BMC Genom, 2017, 18(1): 885.
19	Li T, Wang YH, Huang Y, et al. A novel plant protein-disulfide isomerase participates in resistance response against the TYLCV in tomato [J]. Planta, 2020, 252(2): 25.
20	Andème Ondzighi C, Christopher DA, Cho EJ, et al. Arabidopsis protein disulfide isomerase-5 inhibits cysteine proteases during trafficking to vacuoles before programmed cell death of the endothelium in developing seeds [J]. Plant Cell, 2008, 20(8): 2205-2220.
21	Huang DJ, Chen HJ, Lin YH. Isolation and expression of protein disulfide isomerase cDNA from sweet potato (Ipomoea batatas [L.] Lam ‘Tainong 57’) storage roots [J]. Plant Sci, 2005, 169(4): 776-784.
22	Zhao CF, Luo ZY, Li MZ, et al. Wheat protein disulfide isomerase improves bread properties via different mechanisms [J]. Food Chem, 2020, 315: 126242.
23	Ray S, Anderson JM, Urmeev FI, et al. Rapid induction of a protein disulfide isomerase and defense-related genes in wheat in response to the hemibiotrophic fungal pathogen Mycosphaerella graminicola [J]. Plant Mol Biol, 2003, 53(5): 701-714.
24	Li J, Liu XH, Yang XW, et al. Proteomic analysis of the impacts of powdery mildew on wheat grain [J]. Food Chem, 2018, 261: 30-35.
25	Kan JH, Cai Y, Cheng CY, et al. Simultaneous editing of host factor gene TaPDIL5-1 homoeoalleles confers wheat yellow mosaic virus resistance in hexaploid wheat [J]. New Phytol, 2022, 234(2): 340-344.
26	Jiang WQ, Yang L, He YQ, et al. Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum) [J]. PeerJ, 2019, 7: e8062.
27	李雅倩, 王林, 宋婧含, 等. 小麦NPR1基因家族鉴定及其表达分析 [J]. 南方农业学报, 2021, 52(9): 2339-2349.
	Li YQ, Wang L, Song JH, et al. Genome-wide identification and expression analysis of wheat NPR1 gene family [J]. J South Agric, 2021, 52(9): 2339-2349.
28	Edman JC, Ellis L, Blacher RW, et al. Sequence of protein disulphide isomerase and implications of its relationship to thioredoxin [J]. Nature, 1985, 317: 267-270.
29	李鹏, 刘彻, 宋皓, 等. 烟草非特异性脂质转移蛋白基因家族的鉴定与分析 [J]. 作物学报, 2021, 47(11): 2184-2198.
	Li P, Liu C, Song H, et al. Identification and analysis of non-specific lipid transfer protein family in tobacco [J]. Acta Agron Sin, 2021, 47(11): 2184-2198.
30	Møgelsvang S, Simpson DJ. Changes in the levels of seven proteins involved in polypeptide folding and transport during endosperm development of two barley genotypes differing in storage protein localisation [J]. Plant Mol Biol, 1998, 36(4): 541-552.
31	Kim YJ, Yeu SY, Park BS, et al. Protein disulfide isomerase-like protein 1-1 controls endosperm development through regulation of the amount and composition of seed proteins in rice [J]. PLoS One, 2012, 7(9): e44493.
32	Chen JH, Wang B, Chung JS, et al. The role of promoter cis-element, mRNA capping, and ROS in the repression and salt-inducible expression of AtSOT12 in Arabidopsis [J]. Front Plant Sci, 2015, 6: 974.
33	Wai AH, Waseem M, ABMMM Khan, et al. Genome-wide identification and expression profiling of the PDI gene family reveals their probable involvement in abiotic stress tolerance in tomato (Solanum lycopersicum L.) [J]. Genes, 2020, 12(1): 23.
34	Faheem M, Li YB, Arshad M, et al. A disulphide isomerase gene (PDI-V) from Haynaldia villosa contributes to powdery mildew resistance in common wheat [J]. Sci Rep, 2016, 6: 24227.

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引物名称 Primer name	序列 Sequence （5′-3′）
Actin-F	AGTGGAGGTTCTACCATGTTTCCT
Actin-R	CACTGTATTTCCTTTCAGGTGGTG
TaPDIL1-4A-F	GAGCCTGTGAAGGTAGTTGTGG
TaPDIL1-4A-F	GTGACGAAGTAGAGGGTGGG
TaPDIL2-3B-F	GAGACCACGGTTCTTGCTTACA
TaPDIL2-3B-F	CGATGCCACGGAGATACTTGC
TaPDIL3-7D-F	CCGCTCAGAACCAGTACCAC
TaPDIL3-7D-F	TTCACCGAAGCATCAATACG
TaPDIL4-1B-F	CTGTCAAGCACGGAAAGATC
TaPDIL4-1B-F	AGAGGAGAAGGCTGAAAGAAT
TaPDIL5-5A-F	TTTGGTATTTGGTGCCGATAA
TaPDIL5-5A-F	TTTCTTCCTTCTGCCTTTGAGT
TaPDIL7-6B-F	GTATGAAGTGGTTGAGGGTTTAG
TaPDIL7-6B-F	GGAGCGTCTGCCCAATGTCT

引物名称 Primer name	序列 Sequence （5′-3′）
Actin-F	AGTGGAGGTTCTACCATGTTTCCT
Actin-R	CACTGTATTTCCTTTCAGGTGGTG
TaPDIL1-4A-F	GAGCCTGTGAAGGTAGTTGTGG
TaPDIL1-4A-F	GTGACGAAGTAGAGGGTGGG
TaPDIL2-3B-F	GAGACCACGGTTCTTGCTTACA
TaPDIL2-3B-F	CGATGCCACGGAGATACTTGC
TaPDIL3-7D-F	CCGCTCAGAACCAGTACCAC
TaPDIL3-7D-F	TTCACCGAAGCATCAATACG
TaPDIL4-1B-F	CTGTCAAGCACGGAAAGATC
TaPDIL4-1B-F	AGAGGAGAAGGCTGAAAGAAT
TaPDIL5-5A-F	TTTGGTATTTGGTGCCGATAA
TaPDIL5-5A-F	TTTCTTCCTTCTGCCTTTGAGT
TaPDIL7-6B-F	GTATGAAGTGGTTGAGGGTTTAG
TaPDIL7-6B-F	GGAGCGTCTGCCCAATGTCT

序列号 Sequence ID	基因 Gene	氨基酸数量 Number of amino acids	相对分子量 Relative molecular weight/Da	理论等电点Theoretical pI	不稳定系数Instability index	脂溶指数Aliphatic index	GRAVY	亚细胞定位 Subcellular localization
TraesCS2A02G306500.1	TaPDIL1-2A	517	57 040.81	4.64	37.21	90.87	-0.082	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2B02G323200.1	TaPDIL1-2B	518	57 125.01	4.65	37.84	89.96	-0.083	叶绿体、细胞质Chloroplast， cytoplasm
TraesCS2D02G305000.1	TaPDIL1-2D	517	56 952.69	4.71	35.29	90.14	-0.095	叶绿体、细胞质、核 Chloroplast， cytoplasm， and nucleus
TraesCS4A02G214200.2	TaPDIL1-4A	515	56 579.13	4.99	34.70	85.15	-0.24	内质网Endoplasmic reticulum
TraesCS4B02G101800.3	TaPDIL1-4B	512	56 441.08	5.03	36.30	83.91	-0.249	内质网Endoplasmic reticulum
TraesCS4D02G098400.1	TaPDIL1-4D	515	56 629.18	4.96	33.86	83.44	-0.246	内质网Endoplasmic reticulum
TraesCS3B02G532200.1	TaPDIL2-3B	582	63 587.51	4.61	33.75	89.13	-0.208	叶绿体、核Chloroplast， nucleus
TraesCS6A02G000600.1	TaPDIL2-6A	402	43 760.01	4.58	34.06	88.03	-0.21	叶绿体Chloroplast
TraesCS6B02G005500.1	TaPDIL2-6B	588	63 851.97	4.74	35.34	88.77	-0.172	叶绿体Chloroplast
TraesCS6D02G004700.1	TaPDIL2-6D	588	64 389.68	4.68	33.80	86.66	-0.222	叶绿体Chloroplast
TraesCS6A02G001100.1	TaPDIL2.1-6A	579	63 175.13	4.57	34.68	88.06	-0.21	叶绿体、核Chloroplast， and nucleus
TraesCS6B02G005100.1	TaPDIL2.1-6B	594	64 692.05	4.80	47.11	84.55	-0.349	叶绿体Chloroplast
TraesCS6D02G005200.1	TaPDIL2.1-6D	587	63 940.12	4.59	35.28	88.03	-0.198	叶绿体Chloroplast
TraesCS7A02G123900.1	TaPDIL3-7A	541	59 594.01	4.95	36.86	94.01	-0.157	叶绿体Chloroplast
TraesCS7B02G022800.1	TaPDIL3-7B	555	60 860.45	4.96	33.39	94.99	-0.146	叶绿体Chloroplast
TraesCS7D02G121800.1	TaPDIL3-7D	544	59 621.97	5.03	31.02	92.96	-0.147	叶绿体Chloroplast
TraesCS1A02G097700.1	TaPDIL4-1A	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS1B02G126100.1	TaPDIL4-1B	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS1D02G106500.1	TaPDIL4-1D	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS5A02G234400.1	TaPDIL5-5A	440	47 183.9	5.24	36.60	86.32	-0.145	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS5B02G232900.1	TaPDIL5-5B	440	47 221.96	5.36	37.28	86.09	-0.152	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS5D02G241200.1	TaPDIL5-5D	440	47 174.9	5.30	37.99	86.55	-0.135	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2A02G220100.1	TaPDIL6-2A	415	46 112.8	6.90	40.05	87.55	-0.186	叶绿体Chloroplast
TraesCS2B02G245600.1	TaPDIL6-2B	485	54 407.99	6.90	39.56	87.34	-0.192	叶绿体Chloroplast
TraesCS2D02G225800.1	TaPDIL6-2D	485	54 405.03	6.90	39.71	88.35	-0.172	叶绿体Chloroplast
TraesCS2A02G304300.1	TaPDIL7-2A	413	46 293.1	4.89	39.70	92.28	-0.051	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2B02G321000.1	TaPDIL7-2B	417	46 621.5	4.89	41.42	93.05	-0.047	叶绿体、细胞核 Chloroplast， and nucleus
TraesCS2D02G302900.1	TaPDIL7-2D	451	50 773.16	5.22	46.40	86.03	-0.158	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS6A02G220100.1	TaPDIL7-6A	485	54 391.99	5.17	37.12	94.17	-0.046	叶绿体Chloroplast
TraesCS6B02G261500.2	TaPDIL7-6B	418	46 287.77	4.85	42.74	92.58	-0.077	叶绿体Chloroplast
TraesCS6D02G215100.1	TaPDIL7-6D	417	46 458.32	5.24	37.98	92.78	-0.072	叶绿体Chloroplast

序列号 Sequence ID	基因 Gene	氨基酸数量 Number of amino acids	相对分子量 Relative molecular weight/Da	理论等电点Theoretical pI	不稳定系数Instability index	脂溶指数Aliphatic index	GRAVY	亚细胞定位 Subcellular localization
TraesCS2A02G306500.1	TaPDIL1-2A	517	57 040.81	4.64	37.21	90.87	-0.082	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2B02G323200.1	TaPDIL1-2B	518	57 125.01	4.65	37.84	89.96	-0.083	叶绿体、细胞质Chloroplast， cytoplasm
TraesCS2D02G305000.1	TaPDIL1-2D	517	56 952.69	4.71	35.29	90.14	-0.095	叶绿体、细胞质、核 Chloroplast， cytoplasm， and nucleus
TraesCS4A02G214200.2	TaPDIL1-4A	515	56 579.13	4.99	34.70	85.15	-0.24	内质网Endoplasmic reticulum
TraesCS4B02G101800.3	TaPDIL1-4B	512	56 441.08	5.03	36.30	83.91	-0.249	内质网Endoplasmic reticulum
TraesCS4D02G098400.1	TaPDIL1-4D	515	56 629.18	4.96	33.86	83.44	-0.246	内质网Endoplasmic reticulum
TraesCS3B02G532200.1	TaPDIL2-3B	582	63 587.51	4.61	33.75	89.13	-0.208	叶绿体、核Chloroplast， nucleus
TraesCS6A02G000600.1	TaPDIL2-6A	402	43 760.01	4.58	34.06	88.03	-0.21	叶绿体Chloroplast
TraesCS6B02G005500.1	TaPDIL2-6B	588	63 851.97	4.74	35.34	88.77	-0.172	叶绿体Chloroplast
TraesCS6D02G004700.1	TaPDIL2-6D	588	64 389.68	4.68	33.80	86.66	-0.222	叶绿体Chloroplast
TraesCS6A02G001100.1	TaPDIL2.1-6A	579	63 175.13	4.57	34.68	88.06	-0.21	叶绿体、核Chloroplast， and nucleus
TraesCS6B02G005100.1	TaPDIL2.1-6B	594	64 692.05	4.80	47.11	84.55	-0.349	叶绿体Chloroplast
TraesCS6D02G005200.1	TaPDIL2.1-6D	587	63 940.12	4.59	35.28	88.03	-0.198	叶绿体Chloroplast
TraesCS7A02G123900.1	TaPDIL3-7A	541	59 594.01	4.95	36.86	94.01	-0.157	叶绿体Chloroplast
TraesCS7B02G022800.1	TaPDIL3-7B	555	60 860.45	4.96	33.39	94.99	-0.146	叶绿体Chloroplast
TraesCS7D02G121800.1	TaPDIL3-7D	544	59 621.97	5.03	31.02	92.96	-0.147	叶绿体Chloroplast
TraesCS1A02G097700.1	TaPDIL4-1A	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS1B02G126100.1	TaPDIL4-1B	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS1D02G106500.1	TaPDIL4-1D	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS5A02G234400.1	TaPDIL5-5A	440	47 183.9	5.24	36.60	86.32	-0.145	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS5B02G232900.1	TaPDIL5-5B	440	47 221.96	5.36	37.28	86.09	-0.152	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS5D02G241200.1	TaPDIL5-5D	440	47 174.9	5.30	37.99	86.55	-0.135	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2A02G220100.1	TaPDIL6-2A	415	46 112.8	6.90	40.05	87.55	-0.186	叶绿体Chloroplast
TraesCS2B02G245600.1	TaPDIL6-2B	485	54 407.99	6.90	39.56	87.34	-0.192	叶绿体Chloroplast
TraesCS2D02G225800.1	TaPDIL6-2D	485	54 405.03	6.90	39.71	88.35	-0.172	叶绿体Chloroplast
TraesCS2A02G304300.1	TaPDIL7-2A	413	46 293.1	4.89	39.70	92.28	-0.051	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2B02G321000.1	TaPDIL7-2B	417	46 621.5	4.89	41.42	93.05	-0.047	叶绿体、细胞核 Chloroplast， and nucleus
TraesCS2D02G302900.1	TaPDIL7-2D	451	50 773.16	5.22	46.40	86.03	-0.158	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS6A02G220100.1	TaPDIL7-6A	485	54 391.99	5.17	37.12	94.17	-0.046	叶绿体Chloroplast
TraesCS6B02G261500.2	TaPDIL7-6B	418	46 287.77	4.85	42.74	92.58	-0.077	叶绿体Chloroplast
TraesCS6D02G215100.1	TaPDIL7-6D	417	46 458.32	5.24	37.98	92.78	-0.072	叶绿体Chloroplast

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Identification and Expression Analysis of Protein Disulfide Isomerase Gene Family in Wheat

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